A5003015485- Cancer Genomics and Diagnostics
- Cancer, Lipids, and Metabolism
- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Sarcoma Diagnosis and Treatment
- Testicular diseases and treatments
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Cancer-related gene regulation
- Genetic factors in colorectal cancer
- Circular RNAs in diseases
- Acute Myeloid Leukemia Research
- Cancer-related Molecular Pathways
- Diet and metabolism studies
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Treatments and Mutations
- Cancer Immunotherapy and Biomarkers
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- Bladder and Urothelial Cancer Treatments
- Metabolomics and Mass Spectrometry Studies
- Renal and related cancers
Hospital de Câncer de Barretos
2013-2024
Universidade de São Paulo
2018-2022
Hospital São Paulo
2019
Molecular Oncology (United States)
2013
Universidade Estadual Paulista (Unesp)
2009
Instituto do Câncer do Estado de São Paulo
2008
AC Camargo Hospital
2008
Hospital Alemão Oswaldo Cruz
2007
Ludwig Cancer Research
2006
Universitat Autònoma de Barcelona
2002
We studied 137 primary testicular germ cell tumors (TGCTs) using high-dimensional assays of genomic, epigenomic, transcriptomic, and proteomic features. These exhibited high aneuploidy a paucity somatic mutations. Somatic mutation only three genes achieved significance—KIT, KRAS, NRAS—exclusively in samples with seminoma components. Integrated analyses identified distinct molecular patterns that characterized the major recognized histologic subtypes TGCT: seminoma, embryonal carcinoma, yolk...
Abstract Dysregulation of miRNA expression is associated with multiple diseases, including cancers, in which small RNAs can have either oncogenic or tumor suppressive functions. Here we investigated the potential function miR-450a, one most significantly downregulated miRNAs ovarian cancer. RNA-seq analysis cancer cell line A2780 revealed that overexpression miR-450a suppressed genes involved epithelial-to-mesenchymal transition (EMT). Overexpression reduced migration and invasion increased...
Osteosarcoma (OS) is the most common primary bone cancer in childhood. OS an aggressive disease, and metastatic patients evolve with very poor clinical outcomes. Genetically, OSs are extremely complex tumors, related process not well understood terms of biology disease. In this context, long non-coding RNAs (lncRNAs) have emerged as important class gene expression regulators that play key roles invasion metastasis several human tumors. Here, we evaluated HULC, which lncRNA associated tumor...
Testicular germ cell tumors (TGCT) represent the second main cause of cancer-related death in young men. Despite high cure rates, refractory disease results poor prognosis. Epigenetic reprogramming occurs during development seminomas and non-seminomas. Understanding molecular genetic basis these would an important advance search for new TGCT markers. Hence frequency methylation a gene panel (VGF, MGMT, ADAMTS1, CALCA, HOXA9, CDKN2B, CDO1 NANOG) was evaluated 72 primary by quantitative...
Breast cancer (BC) is a leading cause of cancer-associated mortality in females worldwide. MicroRNAs (miRNAs or miRs), type non‑coding RNA, have been reported to be important the regulation BC onset and progression. Several studies implicated role miR‑183 miR‑494 different types cancer. However, biological functions these miRNAs remain largely unknown. In present study, expression both was assessed MDA-MB-231 MDA‑MB‑468 cell lines. It hypothesized that serve an regulating key genes...
A significant number of genes in mammalian genomes are being found to have natural antisense transcripts (NATs). These sense-antisense (S-AS) pairs believed be involved several cellular phenomena.Here, we generated a catalog S-AS occurring the human and mouse by analyzing different sources expressed sequences available public domain plus 122 massively parallel signature sequencing (MPSS) libraries from variety tissues. Using this dataset almost 20,000 both investigated, computational...
Head and neck squamous cell carcinoma (HNSCC) is associated with environmental factors, especially tobacco alcohol consumption. Most of the carcinogens present in smoke are converted into DNA-reactive metabolites by cytochrome P450 (CYPs) enzymes detoxification these substances performed glutathione S-transferases (GSTs). It has been suggested that genetic alterations, such as polymorphisms, play an important role tumorigenesis HNSCC progression. The aim this study was to investigate CYP1A1,...
LINC00629 and MIR503HG are long intergenic non-coding RNAs (lincRNAs) mapped on chromosome X (Xq26), a region enriched for genes associated with human reproduction. Genes highly expressed in normal reproductive tissues cancers (CT genes) well known as potential tumor biomarkers. This study aimed to characterize the structure, expression, function regulation mechanism of lincRNAs. According our data, expression was almost exclusive placenta other tissues. Further analysis, using cancer cell...
Cancer is a complex disease that can also affect the younger population; however, it responsible for relatively high mortality rate of children and youth, especially in low- middle-income countries (LMICs). Besides that, lipidomic studies this age range are scarce. Therefore, we analyzed blood serum samples from young patients (12 to 35 years) with bone sarcoma (osteosarcoma) compared their lipidomics ones control group samples, named healthy (HC group), using NMR LC-MS techniques....
Osteosarcoma (OS) is the most prevalent type of bone tumor, but slow progress has been achieved in disentangling full set genomic events involved its initiation and progression. We assessed by NGS mutational spectrum 28 primary OSs from Brazilian patients, identified 445 potentially deleterious SNVs/indels 1176 copy number alterations (CNAs). TP53 was recurrently mutated gene, with an overall rate ~60%, considering CNAs. The frequent CNAs (~60%) were gains at 1q21.2q21.3, 6p21.1,...
Testicular germ cell tumors (TGCT) are the most common malignant neoplasm in young men. DNA mismatch repair deficiency can lead to microsatellite instability (MSI), an important mechanism of genetic instability. A mutation BRAF gene has been implicated pathogenesis several solid and recently become therapeutic target. The role MSI TGCT, particularly refractory disease, is poorly understood reported findings controversial. In this study, we aimed determine frequency clinical impact status...
Abstract Background Colorectal cancer (CRC) is one of the most common cancers worldwide; it fourth leading cause death in world and third Brazil. Mutations APC, DCC, KRAS TP53 genes have been associated with progression sporadic CRC, occurring at defined pathological stages tumor consequently modulating several corresponding signaling pathways. Therefore, identification gene signatures that occur each stage during CRC critical can present an impact on diagnosis prognosis patient. In this...
Pediatric osteosarcoma outcomes have improved over the last decades, however, patients that do not achieve a full resection of tumor, even after aggressive chemotherapy, worst prognosis. At genetic level, presents many alterations but there is scarce information on at metabolomic levels. Therefore, an untargeted NMR-metabonomic approach was used to reveal blood serum alterations, when samples taken from twenty-one (21) with aged 12-20 (18, 86%) 43 (3, 14%) before any anticancer therapy were...
The abnormal activation of telomerase, codified by the telomerase reverse transcriptase (TERT) gene, is related to one cancer hallmarks. Hotspot somatic mutations in promoter region TERT, specifically c.-124:C>T and c.-146:C>T, were recently identified a range human cancers have been associated with more aggressive behavior. Testicular germ cell tumors frequently exhibit good prognosis; however, development refractory disease still clinical challenge. In this study, we aim evaluate for first...
Immunophenotyping of bone marrow (BM) hemopoietic precursors is useful for diagnosis adult myelodysplastic syndrome (MDS), but data concerning pediatric patients are limited. We analyzed immunophenotypic features BM cells at children who were referred to the Brazilian Pediatric Cooperative Group Myelodysplastic Syndromes.Diagnosis was based on clinical information, peripheral blood counts, cytology and cytogenetics. Patients with Down excluded. Children deficiency anemias or transitory...
Summary The T‐box transcription factor Brachyury has been considered a cancer‐specific marker and novel oncotarget in solid tumors. overexpression described various cancers, being associated with epithelial–mesenchymal transition, metastasis, poor prognosis. However, its clinical association testicular germ cell tumor is unknown. We analyzed the expression of by immunohistochemistry series well‐characterized samples at transcript level silico analysis. Additionally, we aimed to investigate...
Background: Testicular germ cell tumors (TGCTs), a group of heterogeneous neoplasms, are the most frequent teenagers and young men, with incidence rising worldwide. High cure rates can be achieved through cisplatin (CDDP)-based treatment, but approximately 10% patients present refractory disease virtually no treatment alternatives. Here, we explored new strategies to treat CDDP-resistant. Methods: In vitro TGCT CDDP-resistance model was established differential mRNA expression profiles were...
Despite being a healthy tissue, the constituent cells of placenta, share similar characteristics with tumor cells, such as increased cell growth, migration, and invasion. However, while these processes are stochastic uncontrolled in cancer placenta they precisely controlled. Since miRNAs have been reported to regulate genes that control molecular mechanisms necessary for development both human cancer, we addressed highly expressed could be involved tumorigenesis. Here, assessed miRNA profile...
Sarcomas represent less than 1% of all solid neoplasms in adults and over 20% children. Their etiology is unclear, but genetic susceptibility plays an important role this scenario. Sarcoma central Li-Fraumeni Syndrome (LFS), a familial predisposition cancer syndrome. In Brazil, the high prevalence p.Arg337His mutations TP53 gene brings about unique condition: cluster LFS. present work, we studied 502 sarcoma patients not selected by age or family history attempt to assess impact so-called...
Recent reports have demonstrated that a significant proportion of human genes display allelic differential expression (ADE). ADE is associated with phenotypic variability and may contribute to complex genetic diseases. Here, we present computational analysis using allele-specific serial gene (SAGE) tags representing 1295 genes. We identified 472 for which unequal representation (>3-fold) SAGE was observed in at least one library, suggesting the occurrence ADE. For 235 out these genes,...