A5102809492- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Immune Cell Function and Interaction
- Pancreatic function and diabetes
- thermodynamics and calorimetric analyses
- Endoplasmic Reticulum Stress and Disease
- Nanofabrication and Lithography Techniques
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Galectins and Cancer Biology
- Metabolomics and Mass Spectrometry Studies
- Microbial Inactivation Methods
- bioluminescence and chemiluminescence research
- Microbial Metabolic Engineering and Bioproduction
- Medical Imaging Techniques and Applications
AstraZeneca (United States)
2021-2024
Johns Hopkins University
2018-2024
AstraZeneca (Netherlands)
2024
Bristol-Myers Squibb (United States)
2020
Although process intensification by continuous operation has been successfully applied in the chemical industry, biopharmaceutical industry primarily uses fed-batch, rather than or perfusion methods, to produce stable monoclonal antibodies (mAbs) from Chinese hamster ovary (CHO) cells. Conventional fed-batch bioreactors may start with an inoculation viable cell density (VCD) of ~0.5 × 106 cells/mL. Increasing VCD production bioreactor (referred as N stage bioreactor) 2-10 cells/mL...
Abstract Recent advances in metabolite quantification and identification have enabled new research into the detection control of titer inhibitors promoters. This paper presents a bigdata analytics study to identify both promoters using multivariate data analysis metabolomics data. By applying multi-way partial least squares (PLS) model from four fed-batch bioreactor conditions where feed formulation selection agent concentrations varied, metabolites which exhibited most significant impact on...
The N-glycan pattern of an IgG antibody, attached at a conserved site within the fragment crystallizable (Fc) region, is critical antibody quality attribute whose structural variability can also impact function. For tailoring Fc glycoprofile, glycoengineering in cell lines as well amino acid mutations have been applied. Multiple glycoengineered Chinese hamster ovary were generated, including defucosylated (FUT8KO), α-2,6-sialylated (ST6KI), and (FUT8KOST6KI), expressing either wild-type...
The production of biologics that treat complex diseases, such as cancer, autoimmune, and infectious disease, requires careful monitoring control cell cultures. While bioprocess optimizations have dramatically improved yields, a lack analytical tools has made it challenging to identify accompanying intracellular improvements. Intracellular redox can diminish the growth productivity biologics-producing cells adversely impact product quality profiles yet characterizing is due its highly...
Abstract Chinese Hamster Ovary (CHO) cells are used to produce monoclonal antibodies and other biotherapeutics at industrial scale. Despite their ubiquitous nature in the biopharmaceutical industry, little is known about behaviors of individual transfected clonal CHO cells. Most assessed on ability protein interest over time, as stability. But these have primarily been studied bulk, working under assumption that bulk samples identical because genetic clonality across sample; however, this...
Abstract Chinese hamster ovary (CHO) bioprocesses, the dominant platform for therapeutic protein production, are increasingly used to produce complex multispecific proteins. Product quantity and quality affected by intracellular conditions, but these challenging measure often overlooked during process optimization studies. pH is known impact attributes like aggregation across upstream downstream processes, yet effects of on cell culture performance largely unknown. Recently, advances in...