A5047439124- Viral Infectious Diseases and Gene Expression in Insects
- Chromium effects and bioremediation
- 3D Printing in Biomedical Research
- Microbial Metabolic Engineering and Bioproduction
- Protein purification and stability
- Virus-based gene therapy research
- Wastewater Treatment and Nitrogen Removal
- CAR-T cell therapy research
- thermodynamics and calorimetric analyses
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Insect and Pesticide Research
- Pluripotent Stem Cells Research
- Bone Tissue Engineering Materials
University of Massachusetts Lowell
2020-2023
Cell Biotech (South Korea)
2023
Biocat
2023
Abstract Recombinant adeno‐associated virus (rAAV) vectors are a promising platform for in vivo gene therapies. However, cost‐effective, well‐characterized processes necessary to manufacture rAAV therapeutics challenging develop without an understanding of how process parameters (PPs) affect product quality attributes (PQAs). In this work, central composite orthogonal experimental design was employed examine the influence four PPs transient transfection complex formation...
Abstract Recent advances in metabolite quantification and identification have enabled new research into the detection control of titer inhibitors promoters. This paper presents a bigdata analytics study to identify both promoters using multivariate data analysis metabolomics data. By applying multi-way partial least squares (PLS) model from four fed-batch bioreactor conditions where feed formulation selection agent concentrations varied, metabolites which exhibited most significant impact on...
Trace metals play a critical role in the development of culture media used for production therapeutic proteins. Iron has been shown to enhance productivity monoclonal antibodies during Chinese hamster ovary (CHO) cell culture. However, redox activity and pro-oxidant behavior iron may also contribute toward reactive oxygen species (ROS). In this work, we aim clarify influence trace by examining relationship between supplementation media, mAb glycosylation, oxidative stress interplay within...
Abstract A majority of the biotherapeutics industry today relies on manufacturing monoclonal antibodies from Chinese hamster ovary (CHO) cells, yet challenges remain with maintaining consistent product quality high‐producing cell lines. Previous studies report impact individual trace metal supplemental CHO and thus, combinatorial effects these metals could be leveraged to improve bioprocesses further. three‐level factorial experimental design was performed in fed‐batch shake flasks evaluate...