A5039135104- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolic Engineering and Bioproduction
- Scientific Computing and Data Management
- Enzyme Catalysis and Immobilization
- Advanced Control Systems Optimization
- Simulation Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Manufacturing Process and Optimization
- Business Process Modeling and Analysis
- thermodynamics and calorimetric analyses
- Protein purification and stability
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Biosimilars and Bioanalytical Methods
- Biomedical and Engineering Education
Technische Universität Berlin
2022-2024
Cell Biotech (South Korea)
2023
Biocat
2023
To foster self-driving experimentation and address the reproducibility crisis in bioprocess development a collaborative environment, modular Workflow Management System (WMS) is required. In this work, WMS based on Directed Acyclic Graphs that offers flexible design for plug-and-play integration of computational tools presented. A case study used to demonstrate implementation robotic experimental facilities promotes application Findable, Accessible, Interoperable Re-usable principles,...
Robotic facilities that can perform advanced cultivations (e.g., fed-batch or continuous) in high throughput have drastically increased the speed and reliability of bioprocess development pipeline. Still, developing reliable analytical technologies, cope with cultivation system, has proven to be very challenging. On one hand, accuracy suffers from low sampling volumes, on other number samples must treated rapidly is large. These issues been a major limitation for implementation feedback...
To foster self-driving experimentation and address the reproducibility crisis in bioprocess development a collaborative environment, modular Workflow Management System (WMS) is required. In this work, WMS based on Directed Acyclic Graphs that offers flexible design for plug-and-play integration of computational tools presented. A case study used to demonstrate implementation an experimental-computational robotic experimental facilities promotes application Findable, Accessible, Interoperable...
The fourth industrial revolution in 2011 aimed to transform the traditional manufacturing processes. As part of this revolution, disruptive innovations drug development and data science approaches have potential optimize CMC (chemistry, manufacture, control). real-time simulation processes using “digital twins” can maximize efficiency while improving sustainability. review, we investigate how World Health Organization’s 17 sustainability goals apply toward next-generation development. We...
Increasing the comparability to large scale fermentations is a constant aim during scale-down, including growth-limiting feeding of carbon source. Minibioreactor facilities greatly increase throughput and offer many advantages. However, online measurements in this small often do not include critical process variables, necessary control feed rate. State estimators utilize mathematical models estimate non-measurable states applying existing information about inputs, outputs, measurement...
Miniaturized cultivation systems offer the potential to enhance experimental throughput in bioprocess development. However, they usually lack miniaturized pumps necessary for fed-batch mode, which is commonly employed industrial bioprocesses. An alternative are enzyme-mediated glucose release from starch-derived polymers, facilitating continuous supply. Nevertheless, while release, and thus feed rate, controlled by enzyme concentration, it also strongly depends on type of starch derivative,...
Abstract High-throughput screening accelerates bioprocess development, e.g., drug development. The KIWI-biolab at Technische Universität Berlin developed various platforms for automated experiments in small-scale cultivation systems. These are connected with analytics via a workflow management system and following the FAIR data principles. Mathematical models support process control optimization during screenings of new microbial strains experimental conditions.
Abstract When striving for maximal throughput at minimal volumes while cultivating close to industrial conditions, simple and robust feeding strategies offer important advantages. Enzyme-mediated glucose cleavage from dextrin is an easy way of imitating continuous fed-batch in the small scale, with no complex equipment required. While release rate – thus feed can be controlled by adapting enzyme concentration, it strongly depends on concentration involved substances environmental conditions....