A5086981660- Medical Imaging Techniques and Applications
- Advanced MRI Techniques and Applications
- Nerve injury and regeneration
- Genetic Neurodegenerative Diseases
- Adipose Tissue and Metabolism
- Neuroscience and Neuropharmacology Research
- MRI in cancer diagnosis
- Epigenetics and DNA Methylation
- Optical Imaging and Spectroscopy Techniques
- Mitochondrial Function and Pathology
- Nanoplatforms for cancer theranostics
- Advanced X-ray and CT Imaging
- Intensive Care Unit Cognitive Disorders
- Endoplasmic Reticulum Stress and Disease
- Genetics and Neurodevelopmental Disorders
- Redox biology and oxidative stress
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolism and Genetic Disorders
- Fibroblast Growth Factor Research
- Muscle Physiology and Disorders
- Autism Spectrum Disorder Research
- Anesthesia and Neurotoxicity Research
- Diet and metabolism studies
- DNA Repair Mechanisms
- Genetic Syndromes and Imprinting
Karolinska Institutet
2017-2021
Centro de Biología Molecular Severo Ochoa
2016-2021
Universidad Autónoma de Madrid
2016-2021
Hospital Universitario Puerta de Hierro Majadahonda
2016-2021
Immune-related events in the periphery can remotely affect brain function, contributing to neurodegenerative processes and cognitive decline. In mice, peripheral surgery induces a systemic inflammatory response associated with changes hippocampal synaptic plasticity transient decline, however, underlying mechanisms remain unknown. Here we investigated effect of on neuronal-glial function within neuronal circuits relevance processing male mice at 6, 24, 72 h postsurgery. At 6 detect...
Friedreich's ataxia is a predominantly neurodegenerative disease caused by recessive mutations that produce deficiency of frataxin (FXN). Here, we have used herpesviral amplicon vector carrying gene encoding for brain-derived neurotrophic factor (BDNF) to drive its overexpression in neuronal cells and test effect on FXN-deficient neurons both culture the mouse cerebellum vivo. Gene transfer BDNF primary cultures prevents apoptosis which triggered knockdown FXN expression. This...
Thioredoxin-interacting protein (TXNIP) is an α-arrestin that can bind to and inhibit the antioxidant thioredoxin (TXN). TXNIP expression induced by glucose promotes β-cell apoptosis in pancreas, deletion of its gene mouse models protects against diabetes. currently studied as a potential new target for antidiabetic drug therapy. In this study, we describe family with mutation leading nondetectable protein. Symptoms affected members include lactic acidosis low serum methionine levels. Using...
Energy supply to the brain is essential ensure correct neuronal function, and glucose main fuel utilized by neurons. In metabolically challenging situations when availability restricted, cells may switch alternative carbon substrates. This ensures energy preserve functions of central nervous system. this regard, ketone bodies, a by-product fat metabolism, play key role. They can replace as source ATP in very low, such during fasting, extenuating exercise, or pathological diabetes. However,...
X-ray fluorescence computed tomography (XFCT) with nanoparticles (NPs) as contrast agents shows potential for molecular biomedical imaging higher spatial resolution than present methods. To date the technique has been demonstrated on phantoms and mice, however, parameters such radiation dose, exposure times sensitivityhave not yet allowed high-spatial-resolution in vivo longitudinal imaging, i.e., of same animal at different time points. Here we show XFCT 200-400 μm range a...
Friedreich’s ataxia is the most common hereditary for which there no cure or approved treatment at present. However, therapeutic developments based on understanding of pathological mechanisms underlying disease have advanced considerably, with implementation cellular models that mimic playing a crucial role. Human olfactory ecto-mesenchymal stem cells represent novel model could prove useful due to their accessibility and neurogenic capacity. Here, we isolated cultured these from...
X-ray fluorescence computed tomography (XFCT) with nanoparticles (NPs) as contrast agents has reached technical maturity allowing for in vivo preclinical imaging the laboratory setting. We present first longitudinal study XFCT where mice were 5 times each during an 8-week period. Imaging is performed low radiation dose (<25 mGy) and high signal-to-background high-spatial-resolution (200-400 µm) of molybdenum NP accumulations (down to ~50 µg/ml Mo). further discuss our ongoing development...
Laboratory x-ray fluorescence (XRF) computed tomography (XFCT) with nanoparticles (NPs) as contrast agents now allows for in vivo preclinical imaging and longitudinal studies at low radiation dose. We present on developments of our XFCT arrangement capable low-dose (<25 mGy) high signal-to-background resulting high-spatial-resolution (200-400 μm) Ru, Rh Mo NPs injected accumulated locally mice. further demonstrate multiplexing capabilities by cross-talk-free separation XRF signal well...