A5076860005- Occupational exposure and asthma
- Digestive system and related health
- Occupational Health and Safety Research
- Air Quality and Health Impacts
- Indoor Air Quality and Microbial Exposure
- Contact Dermatitis and Allergies
- Reproductive Physiology in Livestock
- Cancer Cells and Metastasis
- Clinical Nutrition and Gastroenterology
- Cell Adhesion Molecules Research
- Occupational and environmental lung diseases
- Protease and Inhibitor Mechanisms
- Tissue Engineering and Regenerative Medicine
- Estrogen and related hormone effects
- Inflammatory Bowel Disease
- Heavy Metal Exposure and Toxicity
- Immunotoxicology and immune responses
- Helicobacter pylori-related gastroenterology studies
- Nanoparticles: synthesis and applications
- Liver physiology and pathology
- Respiratory viral infections research
- Pancreatic and Hepatic Oncology Research
- Proteoglycans and glycosaminoglycans research
- Receptor Mechanisms and Signaling
- Oral and gingival health research
Health & Safety Laboratory
2010-2025
University of Victoria
2011-2013
Eton College
2012
University of Sheffield
1996-2006
Boston Children's Hospital
2000-2003
Northern General Hospital
2003
Sheffield Children's Hospital
1996-2002
The Royal Free Hospital
2002
University College London
2002
Royal Bournemouth Hospital
2001
An account is given of the many areas science and engineering that are involved in complex fields aircraft missile engineering. The manner which system allows co-ordination these activities into a coherent scheme outlined, together with an indication theoretical tools at disposal System Engineer.A detailed description then “on-line” process control analogue computer novel principles. arithmetic unit consisting minimum number computing amplifiers multipliers used to solve equations piece-wise...
A sound knowledge base is required to target resources reduce workplace exposure carcinogens. This project aimed provide an objective estimate of the burden cancer in Britain due occupation. volume presents extensive analyses for all carcinogens and occupational circumstances defined as definite or probable human by International Agency Research on Cancer. article outlines structure supplement - two methodological papers (statistical approach assessment), eight presenting cancer-specific...
ABSTRACT We describe a reproducible method for growing small intestinal epithelium (derived from the suckling rat intestine) in short-term (primary) cultures. Optimal culture conditions were determined by quantitative assays of proliferation (i.e. changes cellularity and DNA synthesis). Isolation epithelia and, significantly, preservation its three-dimensional integrity was achieved using collagenase/dispase digestion technique. Purification also facilitated use simple differential...
Abstract The changes over short and prolonged periods (up to three months) after castration on the proliferative activity of basal secretory epithelial cells in rat prostate were studied. Although induced widespread apoptosis cells, no compensatory hyperplasia response this was noted. Instead, observations cell kinetics ultrastructure suggested that both entered a quiescent state as result castration. potential not diminished up months During androgen‐induced regeneration pattern...
A variety of enzymic and non-enzymic methods to isolate epithelium from the small intestine have been previously published. Sequential fractionation cells villus crypt has reported in some these papers, which allows comparative study terminally differentiated proliferative cell phenotypes. However, often involve incubation tissues at 37 degrees C, may affect structural biochemical integrity cells. We developed a rapid low-temperature (4 C) method for isolating purified populations mouse rat...
Exposure to metal working fluid (MWF) has been associated with outbreaks of extrinsic allergic alveolitis (EAA) in the USA, bacterial contamination MWF being a possible cause, but is uncommon UK. Twelve workers developed EAA car engine manufacturing plant UK, presenting clinically between December 2003 and May 2004. This paper reports subsequent epidemiological investigation whole workforce. The study had three aims: (1) measure extent outbreak by identifying other who may have or...
The term advanced materials (AM) is used widely to cover a large number of diverse new innovative materials, including nanomaterials, composites, surface coatings, (bio)polymers, porous and particle systems, ceramics, smart metamaterials fibres textiles. With any there are commercial performance advantages that need be balanced with potential environmental, health safety issues, for example, around exposure, toxicity, sustainability waste. Key players in the UK from government bodies,...
Respiratory syncytial virus infects almost all children by 2 years of age. Neutrophils are the predominant airway leucocytes in RSV bronchiolitis and they activated presence infection. However it is not clear whether can directly signal to activate neutrophil cytotoxic function. To investigate this we have used a preparation washed using new centrifugal diafiltration method rapidly remove inflammatory molecules produced epithelial cells propagate stock. Human neutrophils were isolated from...
Inflammatory mediators have been reported to promote malignant cell growth, invasion and metastatic potential. More specifically, we recently that tumour necrosis factor alpha (TNF-α) increases melanoma attachment extracellular matrix (ECM) substrates through fibronectin. In this study, extend these investigations asking specifically whether the TNF-α effect on migration involves activation of proteolytic enzymes. We examined expression/activation type IV gelatinases metalloproteinases 2 9...
Abstract In the prostates of rats ranging from 10 days to 8 months age, proliferative activity basal and secretory epithelial cells was studied. No clear evidence found that alone represented compartment, or they were responsible for replacement during normal turnover. Cell kinetic morphological indicated self‐replicating cell types with discrete functions.
In Wilson's disease and Indian childhood cirrhosis (ICC) copper accumulates in the liver resulting poor hepatocyte regeneration fibrosis. An inhibition of proliferation an increase cell death could account for these outcomes. To establish how toxicity this metal ion impacts upon viability ofthe HepG2 cells they were cultured 4-32 jiM copper(II) sulphate (CuS04)). These levels comparable to circulatory tissue concentrations recorded two diseases. Specific uptake livers patients with ICC was...
Transforming growth factor-beta (TGF-β) can reversibly inhibit the in vitro proliferation of murine and human haemopoietic progenitors and. some their more developmentally restricted progeny. Using an assay for measuring day 8 11 CFU-S, TGF-β caused a gradual decline number CFU-S undergoing DNA synthesis so that after 5 days daily treatment only quiescent cells were found. Release this inhibition was seen within 24 hours post-treatment with recovery all to normal levels. Similar inhibitory...
Abstract. The simultaneous immunohistochemical detection of bromodeoxyuridine (BrdU) and [ 3 H]‐thymidine ([ H]TdR), by conventional autoradiography, was performed on the mouse small intestine (ileum). Proliferation studied under normal conditions as well after Gy γ‐rays. BrdU method in conjunction with H]TdR autoradiography appears to be reliable useful for study cell kinetics especially disturbed states, condition that is delivered animals before BrdU. It has been found cells crypt are...
Summary. Immature female rats (75 g body wt, aged 29 days) were injected with 4 or 40 i.u. PMSG on Day –2 and killed at intervals between 18:00 h 09:00 1. Control animals (4 i.u.) ovulated 00:30 05:30 1 whereas the number of ova recovered from superovulated (40 PMSG) increased slowly 06:00 –1 24:00 0 markedly Similarly treated caged overnight males proven fertility 14:00 16:00 A significantly lower percentage normal 1-cell was compared to control (71·6 98·5% these a fertilized (69·7 99·1%)....
To assess the potential of gingival crevicular fluid (GCF) from adult periodontitis patients and Porphyromonas gingivalis proteases to activate matrix metalloproteinase 2 (MMP-2) in vitro.GCF samples were collected each 15 patients, a clinically healthy site, deep (>6 mm) bleeding nonbleeding site. The GCF examined for general proteolytic activity, gelatinolytic activity ability pro-MMP-2 by zymography. Ultrasonic extracts range clinical isolates P. cells purified arg- lys-gingipains also...
Prelabor rupture of the fetal membranes affects approximately 10% women at term, resulting in an increased risk maternal and neonatal infection. Evidence suggests that membrane is related to biochemical processes involving extracellular matrix membranes. We tested hypothesis prelabor ruptured are characterized by reduced collagen concentrations, altered cross-link profiles, concentrations biomarkers oxidative damage. also set out determine whether these effects modulated ascorbic acid...
Abstract Background Recent work has shown that the chaperone r esistant to i nhibitors of acetyl c holinesterase (RIC-3) is critical for folding, maturation and functional expression a variety neuronal nicotinic acetylcholine receptors. α7 receptors can only assemble functionally express in select lines cells, provided RIC-3 present. In contrast, α4β2 many cell even without presence RIC-3. Depending on line, differential effects receptor function – enhancement mammalian cells but inhibition...