The regeneration of periodontal tissues lost as a consequence destructive disease remains challenge for clinicians. Guided tissue (GTR) has emerged the most widely practiced regenerative procedure. Aim this study was to electrospin chitosan (CH) membranes with low or high degree fiber orientation and examines their suitability use surface layer in GTR membranes, which can ease integration by controlling direction cell growth.A solution CH-doped polyethylene oxide (PEO) (ratio 95:5) prepared...

Guided tissue regeneration (GTR) membranes have been used for the management of destructive forms periodontal disease as a means aiding lost supporting tissues, including alveolar bone, cementum, gingiva and ligaments (PDL). Currently available GTR are either non-biodegradable, requiring second surgery removal, or biodegradable. The mechanical biofunctional limitations currently result in limited unpredictable treatment outcome terms regeneration. In this study, porous chitosan (CH) were...

Tissue engineering approaches have been adapted to reconstruct and restore functionality of impaired tissue for decades. Porous biomimetic composite scaffolds Chitosan (CH) with hydroxyapatite (HA) bone regeneration also extensively studied in the past. These porous play a critical role providing successful by acting as three-dimensional template delivering nutrients metabolites removal waste products. The aim current study was investigate in-vitro in-vivo degradation rates freeze gelated...

The Gram-positive opportunistic pathogen Enterococcus faecalis is frequently responsible for nosocomial infections in humans and represents one of the most common bacteria isolated from recalcitrant endodontic (root canal) infections. E. intrinsically resistant to several antibiotics routinely used clinical settings (such as cephalosporins aminoglycosides) can acquire resistance vancomycin (vancomycin-resistant enterococci).

To assess the impact of periodontal disease and treatment with 24-h root surface debridement on oral health-related quality life patients (OHQoL).Two cohorts were recruited: 20 moderate to advanced 16 dentally healthy patients. Patients treated debridement. OHQoL was assessed, using Oral Health Impact Profile-14, during initial assessment by a telephonic interview daily for 7 days both groups. also assessed at review cohort. The number impacts each patient experienced "occasionally" or more...

The purpose of this study was to relate the numbers Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans cells clinical parameters at diseased healthy periodontal sites before after non-surgical therapy using a sensitive quantitative PCR method (Q-PCR).The sensitivity Q-PCR less than 10 for all three species. Subgingival plaque samples were collected from 541 in 50 adult periodontitis subjects pre-treatment, post-treatment follow-up visit (3-6 months...

Abstract Background/aims: This study aimed to investigate the concentration of cytokine interleukin (IL)‐1β and its receptor antagonist IL‐1ra in gingival crevicular fluid (GCF) patients with adult periodontitis who were heavy smokers compared non‐smokers. Method: GCF samples collected from two groups subjects: Thirty‐nine harvested 13 subjects moderate severe smokers. A further 30 10 Subjects selected both genders none had any relevant systemic illness, pregnant, recent medication or...

Abstract Background/aims: This study aimed to investigate the cytokine IL‐1β and its receptor antagonist IL‐1ra in gingival crevicular fluid (GCF), patients with adult periodontitis. Method: A total of 40 GCF samples were harvested from 10 subjects moderate severe periodontitis healthy controls. Subjects selected both genders, all upper anterior teeth present, no relevant systemic illness, pregnancy or recent medication. All non‐smokers had not received any periodontal therapy within...

To investigate the effects of psychosocial stress on outcome non-surgical periodontal treatment (NPT).Patients were categorized as stressed or unstressed, and degree was measured. One deep bleeding one non-bleeding site ≥6 mm selected in each patient for detailed investigation, clinical parameters recorded before at 6 months after NPT. Elastase C-terminal teleopeptide type I collagen (ICTP) measured gingival crevicular fluid (GCF) samples both intervals.The baseline, biological markers...

Abstract Aim To determine whether combinations of enzymes in gingival crevicular fluid ( GCF ) can act as improved biomarkers compared with single for predicting the outcome treatment and also diagnosing clinical status sites. Methods Thirty subjects chronic periodontitis were recruited to a 12‐month longitudinal pilot study. samples from three representative sites: healthy (≤3 mm), deep non‐bleeding NB (≥6 mm) bleeding DB sites data collected at baseline, 3 months, 6 months 12 following...

Background: An ability to predict the response conventional non‐surgical treatment of a periodontal site would be advantageous. However, biomarkers or tests devised achieve this have lacked sensitivity. The aim study is assess novel combination outcome patients with chronic periodontitis. Methods: Gingival crevicular fluid (GCF) and subgingival plaque were collected from 77 at three representative sites, one healthy (probing depth [PD] ≤3 mm) two diseased (PD ≥6 mm), baseline 3 6 months...

Abstract . This study aimed to detect the levels of osteocalcin in gingival crevicular fluid (GCF) from healthy (≤3 mm sulcus depth and non‐bleeding) diseased sites (≥6 probing bleeding) subjects with adult periodontitis, order further investigate its potential as a possible marker disease process. Periodontal depths, attachment indices were recorded one site each 20 periodontitis. Both GCF accumulated periodontal pocket or sulci flowing into over three‐minute interval sampled. The amounts...

Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans are believed to play an important role in adult periodontitis, but the significance of their relative numbers progress disease is still unclear. Traditional quantitative methods generally time-consuming inaccurate. The aim this study was develop a sensitive, PCR technique that would be useful for enumerating P. Pr. A. subgingival plaque samples from subjects with periodontitis. Primers following genes...

To assess the potential of gingival crevicular fluid (GCF) from adult periodontitis patients and Porphyromonas gingivalis proteases to activate matrix metalloproteinase 2 (MMP-2) in vitro.GCF samples were collected each 15 patients, a clinically healthy site, deep (>6 mm) bleeding nonbleeding site. The GCF examined for general proteolytic activity, gelatinolytic activity ability pro-MMP-2 by zymography. Ultrasonic extracts range clinical isolates P. cells purified arg- lys-gingipains also...

Abstract Aim: (1) To determine the plaque inhibition properties of two formulations alcohol‐free mouthwash [0.1% w/w cetylpyridinium chloride (CPC) (B) and 0.05% CPC (A)] versus a placebo (C). (2) compare plaque‐inhibiting activity between these new mouthwashes. Material Methods: A double‐blind, crossover study with three 1‐week periods was used. Subjects were randomly assigned to one following groups. Group 1 ( n =10) received mouthwashes A, C B in 1, 2 3, respectively, group =11) order B,...

The aim of this 5-year follow-up study was to evaluate clinically and radiographically the long-term results after guided tissue regeneration (GTR) therapy infrabony defects using non-resorbable bioabsorbable barriers.Thirty-one patients with periodontitis 50 that had been treated GTR were recruited. Eleven expanded polytetrafluoroethylene membranes 39 barriers. At baseline 6 60 +/- 3 months surgery, clinical parameters standardized radiographs obtained. During surgery thereafter, distance...

Cell-based therapy using autologous cells has been suggested as a potential approach for periodontal tissue regeneration. Spheroid systems are form of three-dimensional cell culture that promotes matrix interaction, which could recapitulate the aspect homeostasis in vivo. The aim this study is to assess interaction fibroblast spheroids with synthetic and collagen-based membranes have used guided regeneration.Commercially available normal human ligament fibroblasts were grown spheroid forms...

Abstract: Aim: Gingival inflammation may be caused by injury or plaque‐related diseases and reduction in can a useful indicator of gingival recovery. There has been little research on development non‐index methods to measure condition. The aims the study were investigate reliability measurement changes redness swelling, using image analysis, compare this approach with an established method for assessing overgrowth [ J Clin Periodontol 28 (2001) 81 ]. Method: Twenty volunteers recruited...