A5056338620- Liver Disease and Transplantation
- Liver Disease Diagnosis and Treatment
- Vitamin K Research Studies
- Alcohol Consumption and Health Effects
- Vitamin C and Antioxidants Research
- Oral microbiology and periodontitis research
- Hormonal Regulation and Hypertension
- Vitamin D Research Studies
- Anesthesia and Neurotoxicity Research
- Bone health and osteoporosis research
- Organ Transplantation Techniques and Outcomes
- Peroxisome Proliferator-Activated Receptors
- Clinical Nutrition and Gastroenterology
- Eicosanoids and Hypertension Pharmacology
- Drug-Induced Hepatotoxicity and Protection
- Renal function and acid-base balance
- Pharmacological Effects of Natural Compounds
- Antimicrobial Peptides and Activities
- Bioactive Compounds and Antitumor Agents
- Nitric Oxide and Endothelin Effects
- Ion channel regulation and function
- Exercise and Physiological Responses
- Diet, Metabolism, and Disease
- Cardiovascular and exercise physiology
- Temporomandibular Joint Disorders
UiT The Arctic University of Norway
2022-2025
University Hospital of North Norway
2005-2022
Royal Veterinary College
2014-2021
Newcastle upon Tyne Hospital
2013
University College London
1996-2010
Newcastle upon Tyne Hospitals NHS Foundation Trust
2009-2010
Royal Victoria Infirmary
2001-2006
The Royal Free Hospital
2005
Max Delbrück Center
2005
Maastricht University
2005
Mortality in patients with alcoholic hepatitis (AH) remains high, and although corticosteroids are widely used for treatment, the results vary considerably. In AH, neutrophils primed infiltrate liver to produce injury, but paradoxically, main cause of death such is infection. Our prospective study addressed this paradox on one hand increased risk infection other. We hypothesized that full activation by a humoral factor as endotoxin renders them unable respond further bacterial challenge....
The pathophysiological basis of acute-on-chronic liver failure (ACLF) is unclear but systemic inflammatory response thought to be important. In patients with ACLF, the molecular adsorbents recirculating system (MARS) improves individual organ function, effect MARS on proposed mediators unclear. present study was designed determine cytokine profile, oxidative stress, nitric oxide, and ammonia. A total 18 alcohol-related ACLF due inflammation-related precipitants were randomized receive...
Albumin concentration is diminished in patients with liver failure. infusion improves survival of cirrhotic spontaneous bacterial peritonitis, and it hypothesized that this may be due part to its detoxifying capabilities. The aim study was perform detailed quantitative qualitative assessment albumin function cirrhosis. Healthy controls acute deterioration cirrhosis requiring hospital admission (n = 34) were included. assessed using affinity the fatty acid binding sites a spin label (16...
Abstract We measured the serum levels of phylloquinone (vitamin K1) and menaquinones, MK-7 MK-8, in a group 51 women with mean age 81 years who were studied within few hours after hip fracture. A 38 healthy age-matched randomly chosen from same population served as controls. Patients fracture had marked reduction vitamin K1 (336 ± 302 versus 585 490 pg/ml, p < 0.01), (120 84 226 178 0.001), MK-8 (89 113 161 145 large number undetectable levels, especially MK-8. Vitamin K not...
This study explores the hypothesis that inflammatory response induced by administration of lipopolysaccharide (LPS) exacerbates brain edema in cirrhotic rats; and if so whether this is associated with altered metabolism ammonia or anatomical disturbance blood-brain barrier. Adult Sprague-Dawley rats 4 weeks after bile duct ligation (BDL)/Sham-operation, naïve fed a hyperammonemic diet (HD), were injected LPS (0.5 mg/kg, intraperitoneally) saline, killed 3 hours later. increased water HD,...
Hyperammonemia is a feature of liver failure, which associated with increased risk infection. The aims the present study were to determine in vitro, rats fed an ammoniagenic diet and patients cirrhosis, whether induction hyperammonemia results neutrophil dysfunction. As produces cell swelling, we explored role osmoregulating, p38 mitogen-activated protein kinase (p38(MAPK)) pathway mediating this Neutrophils isolated from blood healthy volunteers incubated either 75 microM ammonia or...
The role of proinflammatory cytokines in the pathogenesis portal hypertension is unclear.This study tests hypothesis that tumour necrosis factor alpha (TNF-alpha) an important mediator circulatory disturbances alcoholic hepatitis (AH) and evaluates acute short term effect a single infusion monoclonal chimeric anti-TNF-alpha antibody (Infliximab) on systemic haemodynamics 10 patients with severe biopsy proven AH. Cardiovascular haemodynamics, hepatic venous pressure gradient (HVPG), renal...
Previous studies suggest reduced hepatic endothelial nitric oxide synthase activity contributes to increased intrahepatic resistance. Asymmetric dimethylarginine (ADMA), an endogenous inhibitor, undergoes metabolism via dimethylarginine-dimethylamino-hydrolase, and is derived by the action of protein-arginine-methyltransferases. Our study assessed whether ADMA, its stereo-isomer symmetric (SDMA), are in alcoholic hepatitis patients, determined any relationship with severity portal...
Abstract Treatment of hyperammonemia and hepatic encephalopathy in cirrhosis is an unmet clinical need. The aims this study were to determine whether L-ornithine phenylacetate/phenylbutyrate (administered as the pro-drug phenylbutyrate) (OP) combined are synergistic produce sustained reduction ammonia by acting a substrate for glutamine synthesis, thereby detoxifying ammonia, phenylacetate excreting ornithine-derived phenylacetylglutamine urine. Sprague-Dawley rats studied 4 weeks after bile...
Increased intracranial pressure (ICP) worsens the outcome of acute liver failure (ALF). This study investigates underlying pathophysiological mechanisms and evaluates therapeutic effect albumin dialysis in ALF with use Molecular Adsorbents Recirculating System without hemofiltration/dialysis (modified, M-MARS).Pigs were randomized into three groups: sham, ALF, + M-MARS. was induced by hepatic devascularization (time = 0). M-MARS began at time 2 ended experiment 6. ICP, arterial ammonia,...
Acute liver failure (ALF) is characterized by rapid progressive organ and poor outcome. The pathophysiology of multiorgan dysfunction in ALF remains unclear but increased systemic inflammatory response believed to be an important determining factor. Asymmetric dimethylarginine (ADMA), endogenous nitric oxide synthase inhibitor, results from proteolysis the a major site for its metabolism. ADMA has been shown independently predict outcome associated with severe dysfunction. In this study, we...
We describe a method for the determination of two major urinary metabolites vitamin K as methyl esters their aglycone structures, 2-methyl-3-(3′-3′-carboxymethylpropyl)-1,4-naphthoquinone (5C-aglycone) and 2-methyl-3-(5′-carboxy-3′-methyl-2′-pentenyl)-1,4-naphthoquinone (7C-aglycone), by HPLC with electrochemical detection (ECD) in redox mode. Urinary salts were removed reversed-phase (C18) solid-phase extraction (SPE), predominantly conjugated hydrolyzed methanolic HCl. The resulting...
A new preparative method for isolating homogeneous cardiotoxins from cobra venoms is described. The technique, based on reverse‐phase high‐pressure liquid chromatography, was used to isolate eight of known sequence four different venoms. In each case the found be particularly efficient at removing trace quantities contaminating phospholipase. Cardiotoxins isolated in this manner were retain their full biological activity. Without exception purified lacked powerful haemolytic activity...
Abstract . This study aimed to detect the levels of osteocalcin in gingival crevicular fluid (GCF) from healthy (≤3 mm sulcus depth and non‐bleeding) diseased sites (≥6 probing bleeding) subjects with adult periodontitis, order further investigate its potential as a possible marker disease process. Periodontal depths, attachment indices were recorded one site each 20 periodontitis. Both GCF accumulated periodontal pocket or sulci flowing into over three‐minute interval sampled. The amounts...