A5069217543- Immune Cell Function and Interaction
- Adipokines, Inflammation, and Metabolic Diseases
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Diabetes and associated disorders
- Adipose Tissue and Metabolism
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Mesenchymal stem cell research
Universität Innsbruck
2017-2020
BackgroundThe bone marrow (BM) is a major reservoir of resting memory T cells and long-lived plasma cells, capable providing protection against recurrent infections. Whether the age-related accumulation adipose tissue in BM affects functionality maintenance not well understood.MethodsFor first time, we compare human femur (fMAT) subcutaneous white thigh (tsWAT) obtained from same donors. Therefore, used microarrays for comparative global gene expression analysis, employed assays to analyse...
Abstract After repeated antigen exposure, both memory and terminally differentiated cells can be generated within CD8 + T cells. Although, during their differentiation, activated may first lose CD28, CD28 − eventually express CD57 as a subsequent step, population of (DP) identified in the peripheral blood. How this is distinct from (DN) cells, better characterized non‐activated/early‐activated senescent‐like cell subsets currently unknown. Here, RNA expression four isolated human PBMCs was...
Obesity has been associated with chronic inflammation and oxidative stress. Both conditions play a determinant role in the pathogenesis of age-related diseases, such as immunosenescence. Adipose tissue can modulate function immune system secretion molecules influencing phenotype cells. The importance bone marrow (BM) maintenance antigen-experienced adaptive cells documented mice. Recently, some groups have investigated survival effector/memory T human BM. Despite this, whether high body mass...
To precisely characterize CD146 in adipose stromal/progenitor cells (ASCs) we sorted the stromal vascular faction (SVF) of human abdominal subcutaneous white tissue (sWAT) according to cell surface (cs) expression CD146, DLK1 and CD34. This test identified three main SVF populations: ~50% cs-DLK1-/cs-CD34+/cs-CD146- ASCs, ~7.5% cs-DLK1+/cs-CD34dim/+/cs-CD146+ cs-DLK1+/cs-CD34dim/+/cs-CD146- cells. All contained intracellular CD146. Whole mount fluorescent IHC staining small vessels detected...
Antigen-experienced immune cells migrate back to the bone marrow (BM), where they are maintained in BM survival niches for an extended period. The composition of T cell subpopulations changes with age, leading accumulation highly differentiated and a loss naïve cells. While innate also affected by little is known about interactions between different adaptive populations BM. In this study, phenotype function isolated from human peripheral blood (PB) was analysed detail using flow cytometry,...
Multivalent tetanus and diphtheria toxoid containing vaccines belong to the most frequently applied vaccines. However, there is an imbalance in degree of protection against two antigens with insufficient long-term diphtheria, particularly elderly population. We have previously reported a positive correlation between granulocyte macrophage-colony stimulating factor (GM-CSF) production diphtheria-specific antibodies. Therefore, present study we analyzed effects vivo recombinant GM-CSF on...
Abstract Background. Obesity has been associated with chronic inflammation and oxidative stress. Both conditions play a determinant role in the pathogenesis of age-related diseases, such as immunosenescence. Adipose tissue can modulate function immune system secretion molecules influencing phenotype cells. The importance bone marrow (BM) maintenance antigen-experienced adaptive cells documented mice. Recently, some groups have investigated survival effector/memory T human BM. Despite this,...
Abstract Background. Obesity has been associated with chronic inflammation and oxidative stress. Both conditions play a determinant role in the pathogenesis of age-related diseases, such as immunosenescence. Adipose tissue can modulate function immune system secretion molecules influencing phenotype cells. Recently, importance bone marrow (BM) maintenance antigen-experienced adaptive cells documented. Despite this, whether high body mass index (BMI) may affect BM production supporting these...
Abstract Background. Obesity has been associated with chronic inflammation and oxidative stress. Both conditions play a determinant role in the pathogenesis of age-related diseases, such as immunosenescence. Adipose tissue can modulate function immune system secretion molecules influencing phenotype cells. The importance bone marrow (BM) maintenance antigen-experienced adaptive cells documented mice. Recently, some groups have investigated survival effector/memory T human BM. Despite this,...