A5003928937- Multiple Myeloma Research and Treatments
- Protein Degradation and Inhibitors
- Cancer-related molecular mechanisms research
- Cancer Mechanisms and Therapy
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Chronic Lymphocytic Leukemia Research
- Advanced Breast Cancer Therapies
- Bone Tumor Diagnosis and Treatments
- Sarcoma Diagnosis and Treatment
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- interferon and immune responses
- PI3K/AKT/mTOR signaling in cancer
- Insect Resistance and Genetics
- Horticultural and Viticultural Research
- RNA Interference and Gene Delivery
- Biochemical and Molecular Research
- Acute Myeloid Leukemia Research
- HIV/AIDS drug development and treatment
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Cancer-related Molecular Pathways
Baylor College of Medicine
2019-2023
Kansas State University
2018
Therapeutic targeting of CDK7 has proven beneficial in pre-clinical studies, yet the off-target effects currently available inhibitors make it difficult to pinpoint exact mechanisms behind MM cell death mediated by inhibition. Here, we show that expression positively correlates with E2F and MYC transcriptional programs multiple myeloma (MM) patient cells; its selective counteracts activity via perturbation CDKs/Rb axis impairs MYC-regulated metabolic gene signatures translating into defects...
Long noncoding RNAs (lncRNAs) can drive tumorigenesis and are susceptible to therapeutic intervention. Here, we used a large-scale CRISPR interference viability screen interrogate cell-growth dependency lncRNA genes in multiple myeloma (MM) identified prominent role for the miR-17-92 cluster host gene (MIR17HG). We show that an MIR17HG-derived lncRNA, named lnc-17-92, is main mediator of acting microRNA- DROSHA-independent manner. Lnc-17-92 provides chromatin scaffold functional interaction...
Summary Epicuticular waxes provide a hydrophobic barrier that protects land plants from environmental stresses. To elucidate the molecular functions of maize glossy mutants reduce accumulation epicuticular waxes, eight non‐allelic were subjected to transcriptomic comparisons with their respective wild‐type siblings. Transcriptomic identified 2279 differentially expressed ( DE ) genes. Other genes tended be down‐regulated in mutants; by contrast stress‐responsive pathways induced mutants....
Chordomas are rare spinal tumors addicted to expression of the developmental transcription factor brachyury. In chordomas, brachyury is super-enhancer associated and preferentially downregulated by pharmacologic transcriptional CDK inhibition, leading cell death. To understand underlying basis this sensitivity, we dissect regulatory network compare consequences degradation with inhibition. Brachyury defines chordoma landscape autoregulates through binding its super-enhancer, locus forms a...
Artificial miRNA technology enables the generation of siRNAs to regulate expression targeted genes. However, application alter gene is challenging due their instability and requires a means efficiently deliver into host. Here, we report that animal mRNAs can be heterologously expressed stably produced in lettuce. We have modified rice precursors produce lettuce with potential target mouse complement 3 (C3) coagulation factor 7 (CF7). Expression primary mature transgenic lines was confirmed...
Abstract Dysregulated gene programs are a hallmark of cancer, and oncogenic transcription factors (TFs) have emerged as key players that contribute to this cell state. Chordoma is rare cancer derived from deregulation the TF brachyury (encoded by T gene). While normally turned off in development minimally expressed healthy tissues, it remains highly required chordoma. TFs like been challenging drug directly, chordoma represents an ideal system for studying both biology behind transcriptional...
Abstract Chordomas are rare spinal tumors addicted to expression of the developmental transcription factor brachyury. In chordomas, brachyury is super-enhancer associated and preferentially downregulated by pharmacologic transcriptional CDK inhibition leading cell death. To understand underlying basis this sensitivity, we dissect regulatory network compare consequences degradation with inhibition. Brachyury defines chordoma landscape, autoregulates through binding its super-enhancer, locus...
SUMMARY Long noncoding RNAs (lncRNA) can drive the tumorigenesis and be susceptible to therapeutic intervention. To define landscape of therapeutically actionable lncRNA dependencies in multiple myeloma (MM), we coupled our extensive transcriptomic profile with targeted CRISPR interference viability screen identified RNA Regulator Lipogenesis (RROL) as a leading dependency MM. RROL shares its origin microRNA locus MIR17HG, however supports proliferation survival MM cells microRNA- DROSHA-...