A5100656900- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Phytase and its Applications
- Photosynthetic Processes and Mechanisms
- Cancer-related molecular mechanisms research
- Redox biology and oxidative stress
- ATP Synthase and ATPases Research
- Plant nutrient uptake and metabolism
- MicroRNA in disease regulation
- RNA regulation and disease
- Virus-based gene therapy research
- Heat shock proteins research
- Physiological and biochemical adaptations
- Plant responses to elevated CO2
- Brain Tumor Detection and Classification
- Extracellular vesicles in disease
- Plant Molecular Biology Research
- Agricultural Science and Fertilization
- Transgenic Plants and Applications
- Medical Image Segmentation Techniques
- Trace Elements in Health
- Coastal wetland ecosystem dynamics
Shenyang Agricultural University
2024
Children's Nutrition Research Center at Baylor College of Medicine
2012-2024
Huazhong Agricultural University
2022-2023
Baylor College of Medicine
2011-2022
United States Department of Agriculture
2011-2022
Agricultural Research Service
2011-2022
University of Chicago
2011
Argonne National Laboratory
2011
Dartmouth College
2011
Zhejiang University
2010
In response to iron (Fe) deficiency, dicots employ a reduction-based mechanism by inducing ferric-chelate reductase (FCR) at the root plasma membrane enhance Fe uptake. However, signal pathway leading FCR induction is still unclear. Here, we found that Fe-deficiency-induced increase of auxin and nitric oxide (NO) levels in wild-type Arabidopsis (Arabidopsis thaliana) was accompanied up-regulation activity expression basic helix-loop-helix transcription factor (FIT) ferric reduction oxidase 2...
The mechanisms of delivery plant small RNAs to consumers must be investigated in order harness this technology positively impact biotechnology. Two groups have used honeysuckle (Lonicera japonica) feeding regimes detect a plant-based RNA, termed MIR2911, sera. Meanwhile, numerous failed dietary consumers. Here we catalog levels MIR2911 different herbs, and suggest that particular herb are elevated. Feeding these herb-based diets mice, found the sera urine were associated with intake levels....
Inconsistent detection of plant-based dietary small RNAs in circulation has thwarted the use RNA therapeutics. Here we demonstrate mice consuming diets rich vegetables displayed enhanced serum levels plant specific MIR2911. Differential centrifugation, size-exclusion chromatography, and proteinase K treatment extracts suggest this resides within a K-sensitive complex. Plant derived MIR2911 was more bioavailable than synthetic RNA. Furthermore, exhibited unusual digestive stability compared...
Abstract The ability to alter nutrient partitioning within plants cells is poorly understood. In Arabidopsis (Arabidopsis thaliana), a family of endomembrane cation exchangers (CAXs) transports Ca2+ and other cations. However, experiments have not focused on how the distribution calcium (Ca) elements seeds are altered by perturbed CAX activity. Here, we investigate Ca abundance in seed from cax1 cax3 loss-of-function lines expressing deregulated CAX1 using synchrotron x-ray fluorescence...
A plant's oxygen supply can vary from normal (normoxia) to total depletion (anoxia). Tolerance anoxia is relevant wetland species, rice (Oryza sativa) cultivation, and submergence tolerance of crops. Decoding transmitting calcium (Ca) signals may be an important component tolerance; however, the contribution intracellular Ca transporters this process poorly understood. Four functional cation/proton exchangers (CAX1-4) in Arabidopsis (Arabidopsis thaliana) help regulate homeostasis around...
Impactful dietary RNA delivery requires improving uptake and enhancing digestive stability. In mouse feeding regimes, we have demonstrated that a plant-based ribosomal (rRNA), MIR2911, is more bioavailable than synthetic MIR2911 or canonical microRNAs (miRNAs). Here mutagenesis was used to discern if has distinctive sequence aids stability uptake. Various mutations had modest impacts while one scrambled displayed significantly enhanced stability, serum bioavailability. To assess small (sRNA)...
Summary Increasing populations and temperatures are expected to escalate food demands beyond production capacities, the development of maize lines with better performance under heat stress is desirable. Here, we report that constitutive ectopic expression a heterologous glutaredoxin S17 from Arabidopsis thaliana ( AtGRXS17 ) can provide thermotolerance in through enhanced chaperone activity modulation stress‐associated gene expression. The thermotolerant had increased protection against...
By identifying the relationship between calcium location in plant cell and nutrient bioavailability, characteristics leading to maximal absorption by humans can be identified. Knowledge of cellular molecular targets controlling plants is emerging. These insights should allow for better strategies increasing nutritional content foods. In particular, use preparation-free elemental imaging technologies such as synchrotron X-ray fluorescence (SXRF) microscopy biology may researchers understand...
Transgenic expression of small RNAs is a prevalent approach in agrobiotechnology for the global enhancement plant foods. Meanwhile, emerging studies have, on one hand, emphasized potential transgenic microRNAs (miRNAs) as novel dietary therapeutics and, other, suggested food safety issues if harmful miRNAs are absorbed and bioactive. For these reasons, it necessary to evaluate bioavailability genetically modified crops. As pilot study, two Arabidopsis lines ectopically expressing unique were...
Iron (Fe) is an essential mineral nutrient and a metal cofactor required for many proteins enzymes involved in the processes of DNA synthesis, respiration, photosynthesis. limitation can have detrimental effects on plant growth development. Such are mediated, at least part, through generation reactive oxygen species (ROS). Thus, plants evolved complex regulatory network to respond conditions iron limitations. However, mechanisms that couple deficiency oxidative stress responses not fully...
Energy partitioning and plant growth are mediated in part by a type I H+-pumping pyrophosphatase (H+-PPase). A canonical role for this transporter has been demonstrated at the tonoplast where it serves job-sharing with V-ATPase vacuolar acidification. Here, we investigated whether H+-PPase from Arabidopsis also functions "reverse mode" to synthesize PPi using transmembrane H+ gradient. Using patch-clamp recordings on vacuoles, observed inward currents upon Pi application cytosolic side....
Ochratoxin A (OTA), a common mycotoxin, can contaminate food and feed is difficult to remove. Astaxanthin (ASTA), natural antioxidant, effectively protect against OTA-induced hepatotoxicity; however, its mechanism of action remains unclear. In the present study, we elucidate protective effects ASTA on damage endoplasmic reticulum mitochondria in broiler liver samples by serum biochemical analysis, antioxidant qRT-PCR, Western blot analysis. inhibited expressions ahr, pxr, car, cyp1a1,...
Scope The uptake of dietary plant small RNAs (sRNAs) in consumers remains controversial, which is mainly due to low content combination with poor fractional absorption. MIR2911, among all the sRNAs including microRNAs, has been shown be one most robustly absorbed sRNAs. Here we analyze unusual abundance and unique genesis MIR2911 during vegetable processing. Methods results Using qRT-PCR, increased dramatically macerated tissues while other microRNAs degraded. accumulation correlated...
Multiple Arabidopsis H
The mechanism of high-fat diet (HFD)-induced decrease in erectile function has not been elucidated, and previous studies, spectrin alpha, erythrocytic 1 (SPTA1) is a cytoskeletal protein that regulates cellular function, which belongs to family proteins can affect cell tissue growth development by regulating YAP, an effector on the Hippo signaling pathway, but its particular role elucidated.To explore SPTA1 abnormality induced HFD.We analyzed penile tissues mice normal HFD transcriptomics...
Background: How the distribution and sequestered form of plant macro/micro-nutrients influence their bioavailability, ultimately impact human health, are poorly understood.The legume Medicago truncatula has a portion its tissue calcium (Ca) in Ca oxalate (CaOx) crystal which reduces nutritional value terms bioavailability.The deficient 5 (cod5) mutant total content similar to wild-type (WT) plants, but sequesters less CaOx crystal.Previous shortterm mice feeding studies suggest that this...
Artificial miRNA technology enables the generation of siRNAs to regulate expression targeted genes. However, application alter gene is challenging due their instability and requires a means efficiently deliver into host. Here, we report that animal mRNAs can be heterologously expressed stably produced in lettuce. We have modified rice precursors produce lettuce with potential target mouse complement 3 (C3) coagulation factor 7 (CF7). Expression primary mature transgenic lines was confirmed...
Classical fine-grained image recognition (FGIR) methods mainly concern the class level of a specific dataset. Using these is challenging due to problem small interclass variance and similar features different classes. We propose misclassification-guided two-level partition ViT model (MTP-ViT) its corresponding united loss function (TU-loss) solve above problem. The misclassification distributions all samples showed long-tailed characteristics, which concentrated on classes with more...