Streptococcus suis is a porcine and zoonotic pathogen that causes severe systemic infection in humans pigs. The treatment of S. relies on antibiotics; however, antimicrobial resistance (AMR) an urgent global problem, pushing research attention the surveillance antibiotic-resistant to fore. This study investigated susceptibility 246 strains isolated from diseased pigs Thailand 2018-2020. major sources were lung brain tissues. PCR-based serotyping demonstrated most abundant serotype was 2 or...

Abstract Objectives To define characteristics of Klebsiella pneumoniae complex (hereafter KP) isolates from healthy pigs, farm workers and their household members in Thailand. Methods A total 839 individual rectal swabs pigs on 164 farms 271 faecal samples humans working pig persons living the same Khon Kaen, Thailand were screened for gut colonization by KP. Genomic sequences investigated antibiotic resistance virulence genes. Phylogenetic analyses performed addition to comparison with...

Streptococcus suis is a significant porcine pathogen and zoonotic agent responsible for infectious diseases in humans worldwide. It classified into 29 serotypes, each with varying geographical prevalence pathogenicity. Hence, serotyping of S. crucial active surveillance, outbreak monitoring, infection control. This study developed novel MassARRAY-based single assay to simultaneously identify species differentiate all serotypes. The targeted glutamate dehydrogenase (gdh) recombination/repair...

Antibiotics are freqeuently used in the livestock sector low- and middle-income countries for treatment, prophylaxis, growth promotion. However, there is limited information into zoonotic prevalence dissemination patterns of antimicrobial resistance (AMR) within these environments. In this study we pig farming Thailand as a model to explore AMR; 156 farms were included, comprising small-sized (<50 sows) medium-sized (≥100 farms, where bacterial isolates selectively cultured from...

Streptococcus suis is a zoonotic pathogen causing disease in both animals and humans, the emergence of increasingly resistant bacteria to antimicrobial agents has become significant challenge globally. The objective this study was investigate genetic basis for declining susceptibility penicillin other β-lactams among S. suis. Antimicrobial testing penicillin-binding proteins (PBP1a, PBP2a, PBP2b, PBP2x) sequence analysis were performed on 225 isolated from diseased pigs. This found that...

Background Emergence of Vibrio parahaemolyticus pandemic strain O3:K6 was first documented in 1996. Since then it has been accounted for large outbreaks diarrhea globally. In Thailand, prior studies on and non-pandemic V. had mostly done the south. The incidence molecular characterization strains other parts Thailand have not fully characterized. This study examined seafood samples purchased Bangkok collected eastern characterized isolates. Potential virulence genes, VPaI-7, T3SS2, biofilm...

Streptococcus suis is a significant bacterial pathogen in the swine industry and represents zoonotic threat to human health. This study aimed investigate prevalence, serotype distribution, pathotypic characteristics of S. isolates obtained from nasopharyngeal swabs slaughtered pigs high‐density farming region Thailand. Among 322 swab samples, 194 samples (60.25%) were found harbor . The most prevalent was 8 (7.98%), followed by 19 (7.56%), 29 (6.72%), 3 (5.88%), 2 (5.04%), with 39.92%...

The overall aim of the current study was to test hypotheses that (i) antibiotic resistance in bacteria were more frequent clinically health pigs intensified company owned, medium-scale farms (MSFs) (100–500 sows) than family-owned, small-scale (SSFs) (1–50 and (ii) farmers working at MSFs prone attain resistant SSFs. conducted North-Eastern Thailand, comprising fecal Escherichia coli isolates from pigs, with (contact humans) persons living same household as farmer (non-contact 51 113 Samples...

In veterinary medicine, colistin has been widely used as therapeutic and prophylactic agent, for growth promotion. However, re-introduced into treatment of human MDR bacterial infections. We assessed the characteristics spread plasmid-borne resistance among healthy pigs, workers with animal-contact their household members in Thailand.WGS MIC data 146 mcr-positive isolates from a cross-sectional One Health study were analysed. Long-read sequencing conjugation performed selected...

Understanding the patterns and drivers of antibiotic use in livestock is crucial for tailoring efficient incentives responsible antibiotics. Here we compared routines between pig farms two different levels intensification Khon Kaen province Thailand. Among 113 family-owned small-scale (up to 50 sows) interviewed did 76% get advice from pharmacy about how antibiotics 84% used it primarily treating disease. 51 medium-scale-farms (100–500 belonging companies 100% company’s veterinarian...

In Thailand, pig production has increased considerably in the last decades to meet a growing demand for pork. Antimicrobials are used routinely intensive treat infections and increase productivity. However, use of antimicrobials also contributes rise antimicrobial resistance with potential consequences animal human health. Here, we quantify association between rates extensive farms focus on geographic proximity farm drugstores. Of 164 enrolled farms, 79% reported using disease prevention,...

Twelve nonreplicate carbapenemase-negative ertapenem (ETP)-nonsusceptible (CNENS) Escherichia coli isolates obtained at a Thai university hospital between 2010 and 2014 were characterized compared with 2 carbapenemase-producing E. from the same hospital. Eight unique pulsed-field gel electrophoresis patterns obtained. All produced CTX-M-15 β-lactamase either coexpressed CMY-2 cephalosporinase or showed increased efflux pump activity. Amino acid sequence analysis revealed that an OmpF defect...

We modified rapid polymyxin Nordmann-Poirel (RPNP) test, called colistin disk elution (RCDE) for detecting resistance in Gram-negative bacilli and evaluated its performance compared with broth (CBDE) test recommended by Clinical Laboratory Standards Institute (CLSI). The RCDE was performed using a 10-μg 2.7 mL volume (final concentration of 3.7 μg/mL) either cation-adjusted Mueller-Hinton or phenol red base media bacterial inoculum 1-μL loop, 1-4 16-20 hr incubation Enterobacteriaceae...

Abstract Thailand is undergoing rapid intensification of livestock production where small subsistence farms and medium sized commercial coexist. In farms, antimicrobials are prescribed by a veterinarian, whereas in antimicrobial use remains largely unsupervised. The impact these differences as well other farming practices on the emergence composition resistance genes (ARGs) unknown. We analyzed 363 genomes extended-spectrum ß-lactamase producing (ESBL) and/or AmpC Escherichia coli recovered...

<title>Abstract</title> The advent of Oxford Nanopore Technologies has undergone significant improvements in terms sequencing costs, accuracy, and read lengths, making it a cost-effective, readily accessible approach for analyzing microbial genomes. A major challenge bacterial whole genome by technology is the requirement higher quality quantity high molecular weight DNA compared to short-read platforms. In this study, using eight pathogenic bacteria, we evaluated quality, quantity,...

The advent of Oxford Nanopore Technologies has undergone significant improvements in terms sequencing costs, accuracy, and read lengths, making it a cost-effective, readily accessible approach for analyzing microbial genomes. A major challenge bacterial whole genome by technology is the requirement higher quality quantity high molecular weight DNA compared to short-read platforms. In this study, using eight pathogenic bacteria, we evaluated quality, quantity, fragmented size distribution...