In granulosa cells labeled to isotopic steady-state with [3H]serine, addition of interleukin-1 beta (IL1 beta) or bacterial sphingomyelinase (SMase) induced a rapid decrease (approximately 60% by 10 min) in cellular [3H]Sphingomyelin content and prolonged generation (up 60 [3H]ceramide, the immediate lipid-moiety generated response sphingomyelin hydrolysis. FSH-treated cells, IL1 (0.3-30 ng/ml) inhibited progesterone biosynthesis dose-dependent manner, an effect that was also observed...

Stimulation of [3H]serine-labeled A431 cells with tumor necrosis factor-alpha (TNFalpha) or bacterial sphingomyelinase (SMase) resulted in a rapid decrease (approximately 50% by 15 min) cellular [3H]sphingomyelin content and generation the lipid moiety [3H]ceramide, which remained elevated 60 min later. Sphingomyelin hydrolysis response to TNFalpha SMase time-dependent phosphorylation state c-Jun protein, an effect that was also observed treated membrane-permeable ceramide analogue...

In [3H]serine-labelled granulosa cells treatment with TNF alpha (10 ng/ml) resulted in a transient decrease cellular [3H]sphingomyelin and generation of [3H]ceramide that remained elevated 60 min later. labelled [methyl-14C]choline, induced similar reduction [14C]sphingomyelin content was accompanied by sustained elevation [14C]phosphorylcholine levels. FSH-primed cells, inhibited P450-AROM activity dose-dependent manner, an effect also observed treated bacterial sphingomyelinase (SMase...

Systemic or intratesticular release of TNF␣ and IL1␤ have been implicated in the reduced testosterone biosynthesis impaired production competent spermatozoa found human patients suffering from sepsis chronic inflammation.Although vitro vivo studies demonstrated that intercept hypothalamic-pituitary testis axis at different levels, site(s) action relative contribution each cytokine to overall testicular failure associated systemic inflammatory processes remains poorly defined.In this study we...

This study assessed the effect of different semen storage temperatures and influence pooling in viability. In experiment 1, samples (n = 30) five Majorera bucks were individually processed [Individual (IS)] after first dilution (Tris-yolk extender), semen-diluted aliquots from each male pooled (PS). Thereafter, (IS PS) preserved as fresh (37 20°C), chilled (4°C) frozen semen. Sperm motility percentage abnormal sperm cells intact membrane acrosomes defined. Semen preservation at 20 4°C did...

Contents This study assessed the efficacy of aglepristone at inducing parturition in pregnant goats. Six experimental groups were defined: group A‐5 (n = 12), A‐3.3 A‐2.5 12) and A‐1.5 which goats injected SC once with 5.0, 3.3, 2.5 1.5 mg per kg body weight goat, respectively, L 11), was treated IM 3.75 luprostiol; Ct 1 ml saline solution. Different parameters associated thereafter investigated. In addition, plasma progesterone concentrations defined after treatments till parturition....

Contents This study assessed the effects of different incubation temperatures on semen viability and influence pooling longevity. In experiment 1, samples were collected from five dogs, individually processed (individual semen: IS) then aliquots each male pooled (pooled PS). Semen (IS PS) diluted in a Tris‐glucose‐yolk extender preserved as fresh (37 25°C) chilled (4°C). Sperm motility percentages sperm abnormalities acrosome membrane integrity for 24 h. Storage at 25 or 4°C first h yielded...

Contents The aim of this study was to determinate the semen quality frozen–thawed samples that were chilled for up 2 days before freezing. ejaculates (n = 18) from six dogs collected, pooled and divided into aliquots. first aliquot (C, control) frozen in liquid nitrogen using a conventional protocol reach final concentration 100 × 10 6 spermatozoa/ml, 20% egg yolk 5% glycerol. remaining five aliquots diluted with extender (Tris‐glucose yolk) cooled at 4°C as follows: R1, 1 h; R6, R12, 12...

The synthesis of estradiol by the granulosa cells is a prominent event in ovarian physiology and depends on expression P450(AROM). FSH induces P450(AROM) as result presence promoter CRE (cAMP response element)-like sequence (CLS). In rodents, LH downregulates aromatase during luteinization an yet undescribed mechanism. cells, increases inducible cAMP early repressor (ICER), isoform CREM (cAMP-responsive element modulator) that represses cAMP-induced transcription. possibility ICER activity...

Pregnant goats were induced to parturition on day 145 of pregnancy, with three different protocols: group Cl (n = 19) was injected intramuscularly (IM) 75 microg the prostaglandin analogue R-Cloprostenol; L 20) treated IM 7.5 mg Luprostiol; L(50) 18) 3.75 Luprostiol (IM); in addition, Group S (Control, n 15) 1 ml saline solution. Thereafter, continuously observed record following parameters: parturition, dystocia incidence, placental delivery and kid maternal survival. Moreover, blood...

The proinflammatory cytokine TNFalpha has important actions at the level of ovary, including inhibition P450 aromatase (P450AROM) activity and secretion inhibin, two proteins that are markers granulosa cell's differentiated status. Because transcription both P450AROM inhibin alpha-subunit can be suppressed in ovary by inducible repressor isoform cAMP-responsive element binding modulator (ICER), we have investigated whether its intracellular messenger ceramide induce ICER expression...

Unilateral testicular enlargement was detected in a 5-years-old domestic ferret during routine sterilization. The right testicle showed two different types of proliferative lesions: (i) round nodules, well demarcated, showing soft yellow tissue; (ii) white firm, with irregular-shaped invaginations. Microscopically, the neoplastic proliferations were identified as an interstitial neoplasm and Sertoli cell tumour, respectively. left small intense atrophy. Clinical evaluation did not show any...

In order to complement previous DNA studies, 81 domestic goats, belonging the Majorera Breed (MB, 105), Semi Wild Goats (SWG, 31) and Tinerfeña (TF 61), were used. Both populations (MB SWG), allocated in same geographical area, differentiated previously by microsatellites analyses, TF is from another island. Thirteen morphological measurements recorded, zoometric indexes calculated profile coat colour evaluated. For all measurements, SWG significantly smaller than MB TF. These results agree...

Abstract To address a possible role of type 1 and 2A serine/threonine protein phosphatases (PP1 PP2A) in regulating granulosa cell hormonal responses, we investigated the effects okadaic acid (OA) on FSH- cAMP-induced steroidogenesis these cells. When added alone (0·01–1 nmol/l), cell-permeant phosphatase inhibitor did not affect progesterone 3β-hydroxysteroid dehydrogenase/Δ 5–4 isomerase (3β-HSD) enzyme activity, whereas when with FSH it dose-dependently augmented (minimal effective dose,...

Contents The influence of sexual stimulation and human chorionic gonadotrophin (hCG) administration on plasma testosterone concentrations was assessed in five male Beagles. Each dog exposed to three experimental treatments: C treatment (Control, no stimulation), hCG (dogs were SC injected with 1000 IU hCG) sexually stimulated (SS) where semen collected from the males. All dogs all treatments, one per week for consecutive weeks, a 1 rest between treatments. Blood samples taken same time...

Contents This short communication reports the clinical, ultrasonographic and histopathological findings in a cat with atresia of uterine cervix mucometra. After 6 months continuous oestrous behaviour, remarkable abdominal enlargement was observed 14‐year‐old queen. A presumptive diagnosis mucometra concluded after ultrasound evaluation based on clinical signs blood analyses. Ovariohysterectomy revealed notable symmetrical distension (4–5 cm diameter) both horns that were filled fluid (690...

Initial biosynthetic radiolabelling experiments with cultured granulosa cells revealed the presence of an oligosaccharide-phosphatidylinositol (glycosyl-phosphatidylinositol; (Ose)nPtdIns) structurally related to (Ose)nPtdIns-lipids isolated from other cell types. Prolactin (PRL) stimulated [3H]glucosamine-(Ose)nPtdIns turnover and rapid generation [3H]myristoyl-diacylglycerol in follicle-stimulating hormone-(FSH)-primed endowed PRL receptors. In parallel performed [3H]myo-inositol-labelled...

ABSTRACT The in-vivo regulatory effect of androgens on steroidogenesis was investigated. Adult (2 to 3 months old) hypophysectomized rats were treated intratesticularly with increasing doses 5α-dihydrotestosterone (DHT; 10–200 μg/100 g body weight) or vehicle (50 μl dimethyl sulphoxide; DMSO) in the contralateral testis. Intratesticular testosterone concentrations extremely low 15–20 days after surgery. Treatment DHT caused a dose-dependent inhibition testicular 3β-hydroxysteroid...