Aims: Here we study the effect of monohydrochloride l-arginine, Nα-lauroyl ethylester (LAE), a cationic preservative derived from lauric acid and arginine, on cell envelopes Salmonella typhimurium Staphylococcus aureus at sub-lethal concentration such as their respective minimal inhibitory concentrations, 32 8 μg ml−1, respectively. Methods Results: Bacterial populations were studied by using transmission electron fluorescence microscopy (TEM FM), flow cytometry (FC) ion-flux across cellular...

Vulvovaginal candidiasis (VVC) is the most common infection caused by Candida albicans and greatly reduces quality of life women affected it. Due to ineffectiveness conventional treatments, there growing interest in research involving compounds natural origin. One such compound curcumin (CUR), which has been proven be effective against this microorganism. However, some CUR's physicochemical properties, especially its low aqueous solubility, make therapeutic application difficult. Thus,...

ABSTRACT Introduction Candida spp. bloodstream infection is a frequent form of mycosis with high mortality rates. Biofilm formation potent virulence factor for albicans and parapsilosis , that confers significant resistance to antifungal agents the innate immune response. Echinocandins such as anidulafungin are new drugs broaden available therapeutic arsenal invasive fungal treatment. Here we evaluated effect anidulafungin, human-Monocytes combined treatment on mature biofilms clinical...

Two genes, accA1 and accA2, with nearly identical nucleotide sequences were cloned from Streptomyces coelicolor A3(2). The deduced amino acid of the product these two genes showed high similarity to BcpA2 Saccharopolyspora erythraea other biotin-containing proteins different organisms assumed be alpha subunit a propionyl-CoA carboxylase. A gene, pccB, encoding carboxyl transferase this enzyme complex was also characterized. Strains disrupted in did not show any change acetyl- or carboxylase...

ABSTRACT Two genes, accB and accE , that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to carboxyl transferase domain several propionyl coezyme A (CoA) carboxylases acyl-CoA (ACCases) actinomycete origin, while AccE shows no significant homology any known protein. Expression in Escherichia coli subsequent vitro reconstitution enzyme activity presence biotinylated protein AccA1 or AccA2 confirmed was subunit an ACCase. The additional...

Candida albicans is the prevalent etiological agent in acute vulvovaginal infection and most severe chronic condition known as recurrent candidiasis (VVC). A critical role of local innate immunity defense pathogenesis vaginal by proposed. The fungal recognition immune receptor an essential step for induction responses including cytokines antimicrobial peptides (AMPs) production host protection. Using TLR2-deficient mice, we characterized early response during VVC. Intravaginal challenge...

Vulvovaginal candidiasis (VVC) and recurrent vulvovaginal (RVVC) are two forms of a disease caused by Candida spp. β-defensin (BD) is one the most important families antimicrobial peptides in female genital tract includes molecules that exert essential local functions as PMN chemoattractant peptides. However, information on their role during murine human VVC RVVC limited. Thus, we analyzed behavior contribution BD1 to response mice model cytokine profile BD3 expression cervicovaginal lavage...

Relationships between azole susceptibility and in vivo response to antifungal therapy a murine model of candidiasis were investigated for Candida albicans isolates sampled from human immunodeficiency virus type 1-positive patients with oropharyngeal candidiasis. The susceptibilities seven clinical two reference strains fluconazole (FCZ) itraconazole (ITZ) determined vitro by the broth microdilution method. Four resistant FCZ ITZ, susceptible both azoles, three ITZ (dissociated resistance)....

La candidemia es la micosis invasora más frecuente en los pacientes internados hospitales de alta complejidad el mundo. infección fúngica sistema nervioso central constituye una complicación potencialmente mortal que agrava pronóstico pacientes. El presente artículo aborda aspectos relevantes sobre las características clínicas esta enfermedad, mecanismos invasión del hongo, respuesta inmunitaria local frente a Candida albicans y impacto defectos genéticos receptores inmunidad innata,...

In recent years, the rise of human fungal infections has been associated to lack early diagnosis, uneffective antifungal therapies and vaccines. Disturbance in immune homeostasis, which can be caused by medical interventions immunosuppression induced disease, are well known as risk factors for these pathologies. Cells innate system equipped with surface cytoplasmic receptors recognition microorganisms called pattern (PRRs). PRRs recognize specific pathogen-associated molecular patterns...

<h3>Background</h3> As vaginal flucytosine is not commercially available, it necessary to prepare this formulation in the Pharmacy Department. <h3>Purpose</h3> To describe preparation of 15.5% (FLUCY15.5), and 17% + amphotericin 3% (FLUAM) creams for use their efficacy treatment Candida glabrata tropicalis. <h3>Materials methods</h3> FLUCY15.5 was prepared as follows: Twenty-eight 500 mg capsules (Ancotil) were opened into a mortar contents crushed. The powder mixed with glycerine form...

Phagocytes, including monocytes/macrophages, play an important role in the host defense during Candida albicans infections. In L-arginine metabolism, balance between activation of two enzymes, inducible Nitric Oxide Synthase (iNOS) and arginase, promotes macrophages alternative metabolic states, while M1 profile is related with protection, M2 favored fungal growth evasion. Our aim was to evaluate effect Amphotericin B (AMB) Fluconazole (FLC) on polarization human monocytes induced by C....

Durante los ultimos anos, el aumento de las infecciones fungicas invasivas en humanos, emerge asociado a la falta diagnostico precoz, terapias antifungicas efectivas y al desarrollo vacunas. Perturbaciones homeostasis inmune debido intervenciones medicas o estados inmunosupresion inducidos por diferentes enfermedades, son conocidos factores riesgo para adquisicion estas micosis. Las celulas del sistema innato estan equipadas con receptores superficie citoplasmaticos que permiten...

Background: Candida albicans is the prevalent etiological agent in acute vulvovaginal candidiasis (VVC) and severe chronic condition known as recurrent VVC. A critical role of local innate immunity defense pathogenesis vaginal infection by proposed. The recognition β-glucans immune receptor Dectin-1 essential during antifungal immunity. β-1,3-glucans exposed on C. wall are synthesized β-1,3-glucan synthase enzyme, whose putative catalytic subunit FKS1. Mutations this domain lead to lower or...

Background: A significant increase in invasive candidiasis caused by species different from C.albicans has been reported. C.parapsilopsis complex include three cryptic species: C. parapsilosis sensu stricto, orthopsilosis and metapsilosis. These differ virulence antifungal susceptibility. Adherence factors are crucial for fungal colonization; biofilms related with persistence hydrolytic enzyme production invasion damage. Our aims were to determine the prevalence of lato patients candidemia...