Punicic Acid (PA) is a polyunsaturated fatty acid that accounts for approximately 70%- 80% of Pomegranate Seed Oil (PSO). PA possesses strong antioxidant, anti-inflammatory, anti-atherogenic effects, and anti-tumorigenic properties. extracts have been shown to anticancer activity in many studies. However, there no evidence the effect PSO on T98 glioblastoma cells. Therefore, present study was first investigate mechanisms induced by cells, which one major compounds extracted from PSO.The...

multifactorial and consists of the activation central nervous system immune cells, cerebral infiltration peripheral upregulation inflammatory cytokines, chemokines, reactive oxygen species (14).Oleocanthal (OC), a phenolic component in virgin olive oil (VOO), has anti-inflammatory antioxidant properties similar to ibuprofen, which is non-steroidal drug (NSAID) (1,3,19).Neuroprotective effects OC against neurodegenerative conditions, such as Alzheimer's disease, have been reported...

We compare the efficacy of intratesticular ozone therapy with intraperitoneal in an experimental rat model. For this purpose, 24 rats were divided into four groups including sham-operated, torsion/detorsion, torsion/detorsion plus (O-IP), and (O-IT). The O-IP group received a 4 mg kg-1 injection ozone, O-IT same epididymally. At h after detorsion, sacrificed orchiectomy materials assessed histopathologically. Spermatogenesis seminiferous tubules damage to Sertoli cells histopathologically...

Local anesthetics are used clinically for peripheral nerve blocks, epidural anesthesia, spinal anesthesia and pain management; large concentrations, continuous application long exposure time can cause neurotoxicity. The mechanism of neurotoxicity caused by local is unclear. Neurite outgrowth apoptosis be to evaluate neurotoxic effects. Mouse neuroblastoma cells were induced differentiate generate neurites in the presence anesthetics. culture medium was removed replaced with serum-free plus...

Bone marrow-derived mesenchymal stem cells (BMSCs) possess self-renewal characteristics that distinguish them from other cell types. Recent studies have focused on the effects of conditioned medium (CM) includes extracellular matrix. Here we examined neuroprotective BMSCs and CM damaged neuroblastoma cells.The were divided into five groups: 1) healthy controls, 2) alone, 3) treated with BMSCs, 4) CM, 5) both CM. Neuroprotective then evaluated based upon levels oxidative stress,...

The integrity of healthy skin plays a crucial role in maintaining physiological homeostasis the human body.Chronic conditions such as diabetes mellitus or peripheral vascular diseases can lead to impaired wound healing.Skin healing purposes focusing on main phases healing, i.e., inflammation, proliferation, epithelialization, angiogenesis, remodeling, and scarring.This is complex process, which dependent many cell types mediators interacting highly sophisticated temporal sequence.Although...

We investigated the potential anticancer effects of oleocanthal (OC) on neuroblastoma cells. Cells were divided into four groups: group 1, cells treated with OC; 2, neurons that differentiated from phosphate-buffered saline(PBS); 3, bone marrow derived neuronal (BMDN) mesenchymal stem (BMSCs) 4, BMDN BMSCs PBS. Groups 2 and 4 control groups. The OC cell viability, oxidative stress, neurite inhibition apoptosis at IC50 dose using MTT analysis, i-NOS e-NOS measurement, neurotoxicity screening...

We investigated lateral thoracic and posterior thigh perforator flaps for viability, vascularization, perfusion apoptosis in a rat model. Wistar albino rats were divided into six groups: artery flap (LTPF) sham, 3 × 2 cm2 LTPF, 6 (PTPF) PTPF, PTPF. Flap viability was determined on postoperative days 1 7. On day 7, photographed their measured using two-dimensional planimeter paper. Tissue samples harvested examination by histology immunohistochemistry. Viability differences statistically...

Ulcerative colitis (UC), one of the inflammatory bowel diseases, has been reported to increase in recent years. Although exact cause is unknown, disruptions molecular pathways are thought trigger UC. We aimed examine distributions glycogen synthase kinase-3beta (GSK-3β), nuclear factor-kappa B (NF-κB) and wingless∕int-1 (Wnt-1) different age groups diagnosed with Patients UC were divided into four according their ages: Group 1, aged 18-30 (n=20); 2, 31-45 3, 46-60 4, 61-75 (n=20). Tissue...

The aim of the study is to determine effect Celecoxib administration, dietary or topical, on expression Ki-67, c-erb-B2, bcl-2 and bax genes in rat tongue by immunohistochemistry methods also tdt-mediated dupt-biotin nick end labeling assay order explore their role malignant transformation proliferation rate, apoptosis rate squamous cell carcinoma. Effects celecoxib carcinogenesis were investigated 40 adult male Sprague Dawley 3-3.5 months rats initiated with 30 ppm 4-nitroquinoline-1-oxide....

The use of medicinal plants as an alternative treatment is a historical process and has been known for long time so that classical can be more effective in wound cancer treatment.The purpose this study was to investigate the effects plant extracts used therapeutic purposes cell lines vitro model vivo experimental animal order obtain information.The plants, olive oil (Oleocanthal), mistletoe (Viscum album), Common Centaury (Centaurium erythraea), Momordica charantia, Inula viscosa, Citrus...

Abstract The proximal caecum in quails consists of lymphoid and non‐lymphoid structures. caecal tonsils the part are units gut‐associated tissue poultry. This study aimed to examine histological characteristics caecum, as well compositions dendritic cells (DCs) antigen‐presenting (APCs) tonsil quails. Tissue sections were stained with Crossman's triple, periodic acid–Schiff, Gordon Sweet's silver, Congo red methyl green‐pyronin dyes, immunohistochemically by streptavidin–biotin–peroxidase...

Objective: To investigate the effects of bone marrow derived mesenchymal stem cell (BMSC) application into rat endometrium on oxidative stress, proliferation and apoptosis. Methods: The female rats selected in estrous cycle were divided three groups (saline, media BMSC group). intrauterine intraperitoneal injections performed using saline (200 μL), culture μL) 1×106 BMSCs/200 μL media, then they mated with male rats. On 7th day pregnancy, uterine samples harvested dyed heamatoxylin-eosin...

Bisphenol-A (BPA) used in the production of plastic materials is a temperature-soluble agent. It also has steroid hormone-like activity; therefore, it poses danger to human health. In our study, we aimed investigate effects BPA on lymph node and spleen male rats exposed this agent during prenatal stage. The pregnant female were divided into four groups: control, sham, low dose (300 µg/kg BPA), high (900 BPA). was dissolved 1 mL corn oil administered every day pregnancy. On 21st 45th after...

Abstract Purpose Thymbra spicata species is a widely used plant, especially in the eastern Mediterranean region, and known to have many health benefits. However, effects of its polysaccharides on tumor cells not been searched. We aimed evaluate biological MCF-7 breast cancer cells. Methods MTT test was performed determine cytotoxicity levels doxorubicin L929 fibroblast The expression VEGF GSK-3β were examined immunocytochemically. For vitro wound healing assay, scratch model created shape...

St. John\'s Wort Oil (JWO) has a sedative property and it is used traditionally for the treatment of depression, neuralgia excitability. JWO been shown to have anticancer activity via apoptosis in glioblastoma cells. However, information on whether effective autophagy mechanism still not known. So, current study was first search T98 glioma Three groups were created with human cells; Group 1: cells no treated (Control group). 2: 3 µl/ml JWO. 3: 6 The cell proliferation, oxidative stress,...

The natural products are promising for anti-proliferative effects in cancer. In this study our aim was to analyze the neurotoxic effect of Origanum minutiflorum O. Schwarz & PH. Davis on neuroblastoma cells vitro conditions. were treated with Oregano oil from and doxorubicin, cytotoxic activities these agents evaluated by MTT assay IC50 doses. immunocytochemistry staining eNOS, iNOS TUNEL performed. results analyzed using Graphpad software one-way-ANOVA. immunoreactivities eNOS increased...

The number of breast cancer cases have increased gradually in women. During treatment the cancer, chemotherapy needs adjuvant agents. Medicinal plants an anticancerogenic effect such cases. Our aim is to search cytotoxic Origanum minutiflorum O. Schwarz & P.H. Davis on cells vitro conditions. For this purpose MDA-MB-231 and MCF-7 cell lines adipose tissue derived mesenchymal stem were used. Oregano oil from was exposed all IC50 dose calculated by MTT assay. immunocytochemical staining eNOS,...

Antiepileptic drugs (AED) are teratogens that confer a risk of various congenital malformations including neural tube defects. Lacosamide (LCM) is novel third-generation AED and its effects on (NT) development remain unclear. Thus, the aim present study was to investigate LCM in early stages NT chicken embryo. Three different doses were applied under embryonic disks embryos after they incubated for 30 hours. Incubation continued an additional 80 hours then all obtained routine histology. In...