Abstract By means of enzymatic digestion with chondroitinase-ABC, a novel unsaturated disaccharide bearing two sulfate residues has been derived from chondroitin squid cartilage. Various data, particularly the formation 2-acetamido-2-deoxy-3-O-(β-d-gluco-4-enepyranosyluronic acid)-4-O-sulfo-d-galactose and its 6-O-sulfate isomer by hydrolysis chondro-6-sulfatase chondro-4-sulfatase, respectively, 2-acetamido-2-deoxy-4,6-di-O-sulfo-d-galactose mild acid hydrolysis, indicate that are located...

Objectives: This study aims to investigate the effect of virtual reality (VR) technology and physical therapy on pain-related fear movement in patients with chronic pain. Methods: randomized controlled trial included 73 All participants underwent measurements using Tampa Scale for Kinesiophobia (TSK), Japanese version World Health Organization Work Performance Questionnaire (WHO-HPQ), Pain Self-Efficacy (PSEQ), Numerical Rating (NRS), Hospital Anxiety Depression (HADS), Catastrophizing...

Electron- or X-ray-induced characteristic X-ray analysis has been widely used to determine chemical compositions of materials in vast research fields. In recent years, X-rays from muonic atoms, which a muon is captured, attracted attention because both beam and muon-induced have high transmission abilities. Here we report the first non-destructive elemental carbonaceous chondrite using one world-leading intense direct current source (MuSIC; MUon Science Innovative Channel). We successfully...

H]Dihydroalprenolol was used to study B-adrenergic binding sites in plasma membranes isolated from rabbit liver.Specific measured at 25 "C as the difference between total and presence of 2 p~ dl-propranolol or 10 1-isoproterenol.Binding saturable stereoselective.The maximum number (&=) 434 * 41 fmol/ mg protein.The K d for this determined by Scatchard analysis 1.39 +. 0.09 mi.This value agreed well with (1.27 f 0.12 m) kinetic analysis.The potency order displacement bound...

Elemental isotopic ratios are measured in various research fields and provide useful information regarding age, origin, geological biological activities, ancient climate, etc. Here, we report a new analysis method without sample destruction using muon-induced characteristic X-rays. We demonstrated this by conducting muon-beam irradiation experiments on two Pb plates with different ratios: natural composition artificially enriched 208Pb. The observed broad X-ray peaks of the Kα line around 6...

Plasma membranes prepared from fat cells of rabbit perirenal adipose tissue were found to retain most (87%) their carbohydrate after delipidation.The sugars the delipidated and amounts present per 100 mg peptide weight were: galactose (1.4 mg), mannose (1.7 fucose (0.6 N-acetylglucosamine (2.4 N-acetylgalactosamine (0.3 N-aeetylneuraminie acid (1.0 ribose (0.4 glucose (0.8 mg).Of these, all but latter two shown be associated with glycoproteins.Polyacrylamide gel electrophoresis in sodium...

Two different types of data acquisition methods, "averaging mode" and "ion-counting mode", have been used in a time-of-flight (TOF) mass spectrometry. The most common method is an averaging mode that sums waveform signals obtained from each flight cycle. While it possible to process many ions arriving at the same TOF one cycle, low-abundance are difficult measure because ion overwhelmed by noises detection system. An ion-counting suitable for such low-concentration ions, but counting loss...

Ontogeny of trimeric GTP-binding regulatory proteins (G-proteins) and their subunits in rabbit liver during neonatal development was studied, by using bacterial-toxin-catalysed ADP-ribosylation membrane proteins, immunoblot analysis to quantify the alpha-subunit (alpha s alpha i) stimulatory (Gs) inhibitory (Gi) G-protein beta-subunit, reconstitution assay with cyc- membranes (from Gs-deficient variant S49 lymphoma cell) measure Gs activity. Under optimal conditions ADP-ribosylation, little...

Simultaneous ion counting and waveform averaging implemented on a field-programmable gate array compiled with high-speed digitizer was applied to ultraperformance liquid chromatography-time-of-flight mass spectrometric analysis of sulfa drugs. Ion carried out by "Peak Detection" (PKD) function that works together signal (AVG). Sulfadimidine (SDD) sulfadimethoxine (SDMX) were measured in human serum (HS) model sample matrix. By using simultaneous PKD AVG acquisition, we observed unified...

Abstract Guanine nucleotide-dependent modulation of agonist binding to the beta-receptor reflects coupling receptor nucleotide regulatory protein. Similarly, guanine stimulation adenylate cyclase can be used as an index between protein and catalytic unit cyclase. Using both approaches we have studied in beta-adrenergic receptor-adenylate system rabbit liver during neonatal development. With [3H]dihydroalprenolol ligand, Bmax was relatively unchanged (200-300 fmol/mg protein) birth end day 1...

A fast scheme is proposed for determining the three-dimensional density distribution of a pure electron plasma under thermal relaxation in Penning trap on basis observation axially integrated distribution. The analysis that includes contribution conducting wall reveals appearance halo surrounding core around midplane. confirmed to approach Penning-type equilibrium Also new temperature determination electrons by analyzing radial profile particles extracted with energy selection. This method...

Research Article| July 01 1972 Chondrosulphatase of squid liver K Atsumi; Atsumi Search for other works by this author on: This Site PubMed Google Scholar Y Kawai; Kawai N Seno; Seno Anno Biochem J (1972) 128 (4): 983–985. https://doi.org/10.1042/bj1280983 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share MailTo Twitter LinkedIn Cite Get Permissions Citation Atsumi, Kawai, Seno, Anno; liver. 1 1972; doi: Download citation file: Ris (Zotero)...

Experimentally observed dynamics of vortex patches, as generated by the diocotron instability in a magnetized pure electron plasma, is examined terms power spectra distributions particles and flux, energy spectrum. The global pattern azimuthal-flux initial ring distribution torn into mesoscopic blobs with local circulation at cost reduction radial shear azimuthal flow. In turbulent stage characterized mergers between blobs, spectrum broadens upward wave-number space while dominant mode...