A5072535519- Fern and Epiphyte Biology
- Plant and animal studies
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- thermodynamics and calorimetric analyses
- Plant Diversity and Evolution
- Plant Parasitism and Resistance
- Species Distribution and Climate Change
- Insect and Arachnid Ecology and Behavior
- Evolution and Genetic Dynamics
- Plant Pathogens and Fungal Diseases
- Seed Germination and Physiology
Duke University
2022-2024
Peking University
2018-2022
As the sister clade of seed plants, ferns are significant materials for plant phylogeny research. However, genomic DNA extraction protocol fern samples like modified CTAB method still lacks robustness. Here, we found that amount and condition pinnae critical gDNA in fern, Adiantum capillus-veneris L. In 500 μl solution, recommended is about 10-20 mg (2-3 pieces). The must be instantly-picked from a cultivated suitable environment. With these factors under control, it highly reproducible to...
ABSTRACT Microbial respiration alone releases massive amounts of Carbon (C) into the atmosphere each year, greatly impacting global C cycle that fuels climate change. Larger microbial population growth often leads to larger standing biomass, which in turns higher respiration. How rising temperatures might influence growth, however, depends on how thermal performance curves (TPCs) governing this may adapt novel environments. This adaptation will turn depend there being heritable genetic...
ABSTRACT Understanding how global warming shapes species evolution within communities is a pressing goal of ecology. Temperature affects interacting and can lead to changes in interactions, but that will alter evolutionary trajectories complex food webs poorly understood. Here we address 1) whether different predators affect prey differentially, 2) the web context which this happens influences evolution, 3) temperature directly, 4) ecological interactions mediate evolution. We use...
ABSTRACT Variability of proteins at the sequence level creates an enormous potential for proteome complexity. Exploring depths and limits this complexity is ongoing goal in biology. Here, we systematically survey human plant high-throughput bottom-up native proteomics data protein truncation variants, where substantial regions full-length are missing from observed product. In humans, Arabidopsis , green alga Chlamydomonas approximately one percent show a short form, which can assign by...
Spores are the primary way of spread and reproduction for ferns, a clade seed-free vascular plants. However, no detailed protocol ferns spore cultivation has been reported yet. Here we provide modified approach axenic fern Adiantum capillus-veneris L., based on Cao's Li's method (Cao, et al., 2010; Li, 2013). Our can be briefly divided into four steps: 1) collect spores; 2) sterilize spores with 5% sodium hypochlorite solution wash twice; 3) incubate in liquid Knop's medium dark five days;...