A5083736712- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Algal biology and biofuel production
- Cellular transport and secretion
- Protist diversity and phylogeny
- Acute Myeloid Leukemia Research
- Endoplasmic Reticulum Stress and Disease
- Plant Reproductive Biology
- Indoor Air Quality and Microbial Exposure
- Coral and Marine Ecosystems Studies
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Cellular Mechanics and Interactions
- Genetic Neurodegenerative Diseases
- Genetic and Kidney Cyst Diseases
- RNA and protein synthesis mechanisms
- Air Quality and Health Impacts
- ATP Synthase and ATPases Research
- Plant-Microbe Interactions and Immunity
- Linguistic Variation and Morphology
- Marine Sponges and Natural Products
- Plant Pathogens and Fungal Diseases
Duke University
2019-2025
Stanford University
2013-2024
Center for Child and Family Health
2024
Rockford University
2019
Kyoto University
2018
University of California, San Francisco
2018
Doshisha University
2016
Sumitomo Seika Chemicals (Japan)
2011
Tokyo University of Science
1981-2010
The University of Tokyo
2003-2010
Chloroplasts are eukaryotic photosynthetic organelles that drive the global carbon cycle. Despite their importance, our understanding of protein composition, function, and spatial organization remains limited. Here, we determined localizations 1,034 candidate chloroplast proteins using fluorescent tagging in model alga Chlamydomonas reinhardtii. The provide insights into functions poorly characterized proteins; identify novel components nucleoids, plastoglobules, pyrenoid; reveal widespread...
Numerous studies in multiple systems support that histone H3 lysine 36 dimethylation (H3K36me2) is associated with transcriptional activation; however, the underlying mechanisms are not well defined. Here, we show H3K36me2 chromatin mark written by ASH1L methyltransferase preferentially bound vivo LEDGF, a mixed-lineage leukemia (MLL)-associated protein colocalizes MLL, ASH1L, and on genome wide. Furthermore, facilitates recruitment of LEDGF wild-type MLL proteins to at key target genes...
Abstract Until recently, it had appeared that the septin family of proteins was restricted to opisthokont eukaryotes (the fungi and animals their close relatives microsporidia choanoflagellates). It has now become apparent septins are also present in several other widely divergent eukaryotic lineages (chlorophyte algae, brown ciliates). This distribution details non-opisthokont sequences appear require major revisions hypotheses about origins early evolution septins.
When exposed to stress such as high seawater temperature, corals and other cnidarians can bleach due loss of symbiotic algae from the host tissue and/or pigments algae. Although environmental conditions that trigger bleaching are reasonably well known, its cellular molecular mechanisms not understood. Previous studies have reported occurrence at least four different for tissue: in situ degradation algae, exocytic release host, detachment cells containing death The relative contributions...
Significance Coral reefs are biodiversity hotspots of great ecological, economic, and aesthetic importance. Their global decline under climate change other stresses makes it urgent to understand the molecular bases their responses stress, including “bleaching,” in which corals' photosynthetic algal symbionts lost, thus depriving host animals a crucial source energy metabolic building blocks. We sought clues mechanisms that cause (or protect against) bleaching by analyzing patterns gene...
Most genes in photosynthetic organisms remain functionally uncharacterized. Here, using a barcoded mutant library of the model eukaryotic alga Chlamydomonas reinhardtii, we determined phenotypes more than 58,000 mutants under 121 different environmental growth conditions and chemical treatments. A total 59% are represented by at least one that showed phenotype, providing clues to functions thousands genes. Mutant phenotypic profiles place uncharacterized into functional pathways such as DNA...
Cytokinesis requires coordination of actomyosin ring (AMR) contraction with rearrangements the plasma membrane and extracellular matrix. In Saccharomyces cerevisiae, new membrane, chitin synthase Chs2 (which forms primary septum [PS]), protein Inn1 are all delivered to division site upon mitotic exit even when AMR is absent. essential for PS formation but not localization. The C-terminal region necessary localization, distinct PXXP motifs in this mediate functionally important interactions...
Abstract Septins are essential for cytokinesis in Saccharomyces cerevisiae , but their precise roles remain elusive. Currently, it is thought that before cytokinesis, the hourglass-shaped septin structure at mother-bud neck acts as a scaffold assembly of actomyosin ring (AMR) and other factors. At onset hourglass splits to form double sandwiches AMR may function diffusion barriers restrict diffusible factors division site. Here, we show cells lacking Cdc10 or septin-associated protein Bud4,...
In yeast and animal cytokinesis, the small guanosine triphosphatase (GTPase) Rho1/RhoA has an established role in formation of contractile actomyosin ring, but its role, if any, during cleavage-furrow ingression abscission is poorly understood. Through genetic screens yeast, we found that either activation Rho1 or inactivation another GTPase, Cdc42, promoted secondary septum (SS) formation, which appeared to be responsible for abscission. Consistent with this hypothesis, a dominant-negative...
Dicentric chromosomes are unstable products of erroneous DNA repair events that can lead to further genome rearrangements and extended gene copy number variations. During mitosis, they form anaphase bridges, resulting in chromosome breakage by an unknown mechanism. In budding yeast, dicentrics generated telomere fusion break at the fusion, a process restores parental karyotype protects cells from rare accidental fusion. Here, we observed lacking preferentially within 25- 30-kb-long region...
The unicellular green alga Chlamydomonas reinhardtii is a model organism that provides an opportunity to understand the evolution and functional biology of lineage includes land plants, as well aspects fundamental core conserved throughout eukaryotic phylogeny. Although many tools are available facilitate genetic, molecular biological, biochemical, cell biological studies in Chlamydomonas, expression unselected transgenes interest (GOIs) has been challenging. In most methods used previously,...
Septins are a family of membrane-associated cytoskeletal guanine-nucleotide binding proteins that play crucial roles in various cellular processes, such as cell division, phagocytosis, and organelle fission. Despite their importance, the evolutionary origins ancestral function septins remain unclear. In opisthokonts, form five distinct groups orthologs, with subunits from multiple assembling into heteropolymers, thus supporting diverse molecular functions. Recent studies have revealed also...
Abstract Actin is one of the most conserved eukaryotic proteins. It thought to have multiple essential cellular roles and function primarily or exclusively as filaments (“F-actin”). Chlamydomonas has been an enigma, because a null mutation (ida5-1) in its single gene for conventional actin does not affect growth. A highly divergent gene, NAP1, upregulated ida5-1 cells, but it unclear whether NAP1 can form provide function. Here, we used actin-depolymerizing drug latrunculin B (LatB),...
Successful cell division requires faithful and segregation of organelles into daughter cells. The unicellular alga Chlamydomonas reinhardtii has a single, large chloroplast whose is spatiotemporally coordinated with furrowing. Cytoskeletal structures form in the same plane at midzone dividing (FtsZ) (microtubules), but how these are not understood. Previous work showed that loss F-actin blocks furrow ingression, suggesting pharmacological perturbations can disorganize events. In this study,...
During yeast sporulation, a forespore membrane (FSM) initiates at each spindle-pole body and extends to form the spore envelope. We used Schizosaccharomyces pombe investigate role of septins during this process. prior conjugation haploid cells, four vegetatively expressed (Spn1, Spn2, Spn3, Spn4) coassemble fusion site are necessary for its normal morphogenesis. Sporulation involves different set (Spn2, Spn5, Spn6, atypical Spn7) that does not include core subunits vegetative septin complex....
Interactions between the dinoflagellate endosymbiont Symbiodinium and its cnidarian hosts (e.g. corals, sea anemones) are foundation of coral-reef ecosystems. Carbon flow partners is a hallmark this mutualism, but mechanisms governing impact on symbiosis remain poorly understood. We showed previously that although strain SSB01 can grow photoautotrophically, it mixotrophically or heterotrophically when supplied with Glc, metabolite normally transferred from alga to host. Here we show Glc...
The multivesicular body (MVB) sorting pathway is required for a number of biological processes, including downregulation cell-surface proteins and protein into the vacuolar lumen. function this requires endosomal complexes transport (ESCRT) composed class E (Vps) in Saccharomyces cerevisiae, many which are conserved Schizosaccharomyces pombe. Of these, sst4/vps27 (homologous to VPS27) sst6 (similar VPS23) have been identified as suppressors sterility ste12Delta (sst), although their...
Significance Studies of eukaryotic cell division have focused on the actomyosin ring, whose filaments F-actin and myosin-II are hypothesized to generate contractile force for ingression cleavage furrow. However, has a very limited taxonomic distribution, whereas by furrowing is much more widespread. We used green alga Chlamydomonas reinhardtii investigate how furrow can form without potential roles in this process. Although was associated with ingressing furrows, its complete removal only...
ABSTRACT Septins are a family of membrane-associated cytoskeletal GTPases that play crucial roles in various cellular processes, such as cell division, phagocytosis, and organelle fission. Despite their importance, the evolutionary origins ancestral function septins remain unclear. In opisthokonts, form five distinct groups orthologs, with subunits from multiple assembling into heteropolymers, thus supporting diverse molecular functions. Recent studies have revealed also conserved algae...