A5017074600- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Porphyrin Metabolism and Disorders
- Traffic control and management
- Vehicle Dynamics and Control Systems
- Amino Acid Enzymes and Metabolism
- Folate and B Vitamins Research
- Biochemical and Molecular Research
- Microbial Natural Products and Biosynthesis
- 3D Printing in Biomedical Research
- Biofuel production and bioconversion
- Biopolymer Synthesis and Applications
- Biochemical and biochemical processes
- Autonomous Vehicle Technology and Safety
- Innovative Microfluidic and Catalytic Techniques Innovation
- Sustainable Industrial Ecology
- Chemical Synthesis and Analysis
- Analytical Chemistry and Chromatography
- Microbial Metabolism and Applications
- Advanced biosensing and bioanalysis techniques
- Electric and Hybrid Vehicle Technologies
- Chemistry and Chemical Engineering
- Viral Infectious Diseases and Gene Expression in Insects
Chongqing University
2020-2025
China Three Gorges University
2023
Tianjin Institute of Industrial Biotechnology
2023
Chinese Academy of Sciences
2023
Abstract Adenosylcobalamin (AdoCbl), a biologically active form of vitamin B 12 (coenzyme ), is one the most complex metal-containing natural compounds and an essential for animals. However, AdoCbl can only be de novo synthesized by prokaryotes, its industrial manufacturing to date was limited bacterial fermentation. Here, we report method synthesis based on cell-free reaction system performing cascade catalytic reactions from 5-aminolevulinic acid (5-ALA), inexpensive compound. More than 30...
Abstract The biosynthesis of cadaverine from lysine is an environmentally promising technology, that could contribute to a more sustainable approach manufacturing bio‐nylon 5X. However, the titer biosynthesized has still not reached sufficient level for industrial production. A powerful green cell factory was developed enhance production by regulating lipopolysaccharide (LPS) genes and improving membrane permeability. Firstly, 10 LPS mutant strains were constructed effect on growth...
δ-Valerolactam (VL), as an organic compound, is important precursor chemical for nylon and has a wide range of applications in synthesis, pharmaceutical polymer materials, other fields. This study introduces novel biosynthetic method producing VL the engineered strain Escherichia coli BL21 through reprogramming polyketide synthases (PKS). Initially, vitro multienzyme system was constructed to verify reliability synthesis pathway. Subsequently, optimized pathway established E. coli,...
<title>Abstract</title> Current methods for evaluating biocarbon fixation efficiency (BCF), such as genome-scale metabolic models and life cycle assessment, lack consideration of reaction atom economy fail to connect genetic relationships with the process. To address these limitations, we introduced an atomic evaluation index centered on enzyme kinetics, named Economic Indicators Real Biological Carbon Fixation Atoms (EIRCBFA), proposed a machine learning-based model assess BCF at both...
l-Pipecolic acid (L-PA), an essential chiral cyclic nonprotein amino acid, is gaining prominence in the food and pharmaceutical sectors due to its wide-ranging biological pharmacological properties. Historically, L-PA has been synthesized chemically for commercial purposes. This study introduces a novel efficient microbial production method using engineered strain
D-Phenyllactic acid (D-PLA) is a potent antimicrobial typically synthesized through chemical methods. However, due to the complexity and large pollution of these reactions, simpler more eco-friendly approach was needed. In this study, strain for D-PLA biosynthesis constructed, but efficiency restricted by activity D-lactate dehydrogenase (DLDH). To address issue, DLDH mutant library constructed Surface-Enhanced Raman Spectroscopy (SERS) employed precise quantification at single-cell level....
Hydrogenobyrinic acid, a modified tetrapyrrole composed of eight five-carbon compounds, is key intermediate and central framework vitamin B12. Synthesis hydrogenobyrinic acid requires S-adenosyl-methionine working as the methyl group donor catalyzed by 12 enzymes including six methyltransferases, causing great shortage accumulation S-adenosyl-homocysteine, which uneconomic unsustainable for cascade reaction. Here, we report cell-free synthetic system producing integrating using...
This paper proposes a cooperative game-based actuator fault-tolerant control strategy for four-wheel independent drive electric vehicle with an active front steering system. For achieving and targets cooperation, two-dimensional game is proposed to balance the stability economy. The first-dimensional utilized determine dominant target of actuator, then second-dimensional employed assign task remaining healthy actuators. two dimensions are integrated based on linear quadratic differential...
1,6-hexamethylenediamine (HMD) is an important precursor for nylon-66 material synthesis, while research on the bioproduction of HMD has been relatively scarce in scientific literature. As concerns about climate change, environmental pollution, and depletion fossil fuel reserves continue to grow, significance producing fundamental chemicals from renewable sources becoming increasingly prominent. In recent investigations, adipic acid reported but with lingering challenges concerning costly...