A5011724316- Lipid metabolism and disorders
- Metabolism, Diabetes, and Cancer
- Liver Disease Diagnosis and Treatment
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- Thyroid Disorders and Treatments
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- Computational Drug Discovery Methods
- Estrogen and related hormone effects
- Metabolomics and Mass Spectrometry Studies
- Alcohol Consumption and Health Effects
- Pancreatic function and diabetes
- Diabetes and associated disorders
- PARP inhibition in cancer therapy
- Receptor Mechanisms and Signaling
- Lipoproteins and Cardiovascular Health
- Virus-based gene therapy research
- Signaling Pathways in Disease
- Acute Lymphoblastic Leukemia research
- Sleep and related disorders
- Advanced biosensing and bioanalysis techniques
- Epilepsy research and treatment
- Sirtuins and Resveratrol in Medicine
Northeast Ohio Medical University
2013-2024
Pharmaceutical Biotechnology (Czechia)
2017
Ohio University
2011
University of Tennessee Health Science Center
2006-2007
University of Tennessee at Knoxville
2006
Crowell & Moring (United States)
2006
The pyruvate dehydrogenase complex (PDC) catalyzes the conversion of to acetyl-CoA in mitochondria and is a key regulatory enzyme oxidation glucose acetyl-CoA. Phosphorylation PDC by kinases (PDK2 PDK4) inhibits activity. Expression PDK genes elevated diabetes, leading decreased In these studies we have investigated transcriptional regulation PDK4 gene estrogen-related receptors (ERRalpha ERRgamma). ERRs are orphan nuclear whose physiological roles include induction fatty acid heart muscle....
A potential means of pharmacological management ischemic stroke is rapid intervention using potent neuroprotective agents. Thyroid hormone (T3) has been shown to protect against damage in middle cerebral artery occlusion (MCAO) model brain stroke. While thyroid permeable across the blood-brain barrier, we hypothesized that efficacy can be enhanced by encapsulation nanoparticulate delivery vehicles. We tested our hypothesis generating poly-(lactide-co-glycolide)-polyethyleneglycol...
Artificial neural networks (ANNs) technology models the pattern recognition capabilities of brain. Similarly to a single neuron in brain, artificial unit receives inputs from many external sources, processes them, and makes decisions. Interestingly, ANN simulates biological nervous system draws on analogues adaptive neurons. ANNs do not require rigidly structured experimental designs can map functions using historical or incomplete data, which them powerful tool for simulation various...
The PGC-1s (peroxisome-proliferator-activated receptor γ co-activators) are a family of transcriptional regulators that induce the expression various metabolic genes. PGC-1 proteins stimulate genes involved in mitochondrial biogenesis, fatty acid oxidation and hepatic gluconeogenesis. Previous studies have demonstrated PGC-1α β isoforms interact with nuclear receptors through conserved LXXLL (leucine-X-X-leucine-leucine) motifs. In present study, we investigated mechanisms by which these...
Thyroid hormone (T3) mediates diverse physiological functions including growth, differentiation, and energy homeostasis through the thyroid receptors (TR). The TR binds DNA at specific recognition sequences in promoter regions of their target genes known as response elements (TREs). Gene expression TREs regulated by TRs is mediated coregulator recruitment to bound receptor. This TR-coregulator interaction controls transcription multiple mechanisms covalent histone modifications chromatin...
Rate constant estimation with heavy water requires a long-term experiment data collection at multiple time points (3-4 weeks for mitochondrial proteome dynamics in mice and much longer other species). When tissue proteins are analyzed, this approach euthanizing animals each point or biopsies humans. Although short-term protocols available, they require knowledge of the maximum number isotope labels (N) accurate quantification observed 2H-enrichment peptide. The high-resolution mass...
Mice fed a high-fat diet for 12 weeks or longer develop hyperglycemia, insulin resistance, dyslipidemia, and fatty liver. Additionally, induces inflammation that remodels affects the anti-inflammatory antiatherogenic property of high-density lipoprotein (HDL). However, precise time course metabolic disease progression HDL remodeling remains unclear. Short-term (four weeks) feeding (60% fat calories) was performed in wild-type male C57BL/6J mice to gain insights into early processes...
Depression guidelines discourage benzodiazepine monotherapy and limit use to short-term adjunctive therapy with antidepressants; however, patients depression continue receive monotherapy. The prevalence predictors of this prescribing pattern have not been described previously are warranted assist clinicians in identifying at highest risk receiving monotherapy.A national, cross-sectional analysis the National Ambulatory Medical Care Survey from 2012 2015 was performed for adults treated...
Hypertriglyceridaemia enhances cardiovascular disease risk in patients with diabetes. Lipoprotein lipase (LPL) regulates plasma triglyceride levels by hydrolysing chylomicrons and very-low-density lipoprotein (VLDL). Metformin, an antidiabetic drug, improves lipids including triglycerides. We examined metformin's regulation of angiopoietin-like 3 (ANGPTL3), a liver-derived secretory protein LPL inhibitory property.Using HepG2 cells, human hepatocyte cell line, the effects metformin on...
Clinical viability of gene delivery systems has been greatly impacted by potential toxicity the systems. Recently, we reported nanoparticle (NP) preparation process that employs biocompatible materials such as Gelucire® 44/14 and cetyl alcohol matrix materials. In current study, NP was modified for pDNA loading through: (i) inclusion cationic lipids (DOTAP or DDAB) with materials; (ii) application surfactants (CTAB) to generate NPs desired surface charges complexation. Colloidal stability...
<b>Abstract ID 128649</b> <b>Poster Board 204</b> Type 2 diabetes mellitus affects hundreds of millions globally, primarily characterized by hyperglycemia. Dyslipidemia, prevalent in 50%-80% diabetic patients, presents as elevated triglycerides and reduced high-density lipoprotein (HDL) cholesterol, exacerbating cardiovascular risks. Metformin, a first-line treatment for type diabetes, effectively lowers glucose levels without hypoglycemia or weight gain, possibly inhibiting gluconeogenesis...
Several aspects of diabetes pathophysiology and complications result from hyperglycemia-induced alterations in the structure function plasma proteins. Furthermore, insulin has a significant influence on protein metabolism by affecting both synthesis degradation proteins various tissues. To understand role progressive hyperglycemia proteins, this study, we measured turnover rates high-density lipoprotein (HDL)-associated control (chow diet), prediabetic [a high-fat diet (HFD) for 8 wk] or...
The 2013 American College of Cardiology/American Heart Association cholesterol guidelines, which identified four groups patients at risk for atherosclerotic cardiovascular disease events, departed from the target-based approach to managing cholesterol. impact these guidelines on high-intensity statin use across United States is unclear.The primary objective was evaluate rate potential (HIP) before and after guidelines. secondary identify predictors HIP within study population.A national...
Elevated triglycerides and decreased HDL cholesterol are hallmarks of metabolic syndrome. Lipoprotein lipase (LPL) found lining the endothelium tissue in adipose tissue, heart muscle is an enzyme that catalyzes hydrolysis triglyceride rich lipoproteins chylomicrons into free fatty acids. Regulation LPL a key physiological mechanism for control lipid levels atherosclerosis risk. Angiopoietin‐like 4 (ANGPTL4) which inhibits activity critical regulatory protein physiology metabolism. Currently,...
T2 (3,5-diiodo-I-thyronine) is an active metabolite of T3 (3,3’,5’-triiodothyronine), a key hormone secreted by the thyroid gland. It has been found to significantly increase mitochondrial respiration and cytochrome oxidizes activity in both vitro vivo studies. much more rapid stimulation metabolic rate (1 hour for vs 24 hours T3). Neuroprotective effects have previously demonstrated. Here, we investigated neuroprotective on brain ischemia edema formation t-MCAO mice model stroke. CD-1...