A5103080376- Cholinesterase and Neurodegenerative Diseases
- Neuroscience and Neuropharmacology Research
- Computational Drug Discovery Methods
- Receptor Mechanisms and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Drug Transport and Resistance Mechanisms
- Nanoparticle-Based Drug Delivery
- Adenosine and Purinergic Signaling
- Glaucoma and retinal disorders
- Click Chemistry and Applications
- Barrier Structure and Function Studies
- Metalloenzymes and iron-sulfur proteins
- Sirtuins and Resveratrol in Medicine
- Alzheimer's disease research and treatments
- Neurotransmitter Receptor Influence on Behavior
- Corneal Surgery and Treatments
- Peroxisome Proliferator-Activated Receptors
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Parkinson's Disease Mechanisms and Treatments
- Lipid metabolism and disorders
- Adipose Tissue and Metabolism
- Amino Acid Enzymes and Metabolism
- TGF-β signaling in diseases
- Nuclear Receptors and Signaling
West Virginia University
2016-2024
Morgantown High School
2024
Northeast Ohio Medical University
2007-2019
Texas Tech University
2005-2013
Texas Tech University Health Sciences Center
2005-2013
National Cancer Institute
2013
Center for Cancer Research
2013
Ohio University
2006-2012
Weatherford College
2011-2012
Northeastern University
2008-2010
Paclitaxel is not effective for treatment of brain cancers because it cannot cross the blood–brain barrier (BBB) due to efflux by P-glycoprotein (P-gp). In this work, glutathione-coated poly-(lactide-co-glycolide) (PLGA) nanoparticles (NPs) paclitaxel were developed targeting cancers. P-gp ATPase assay was used evaluate NP as potential substrates. The showed a particle size suitable BBB permeation (particle around 200 nm) and higher cellular uptake demonstrated in RG2 cells. suggested that...
Brain (central nervous system; CNS) metastases pose a life-threatening problem for women with advanced metastatic breast cancer. It has recently been shown that the vasculature within preclinical brain metastasis model markedly restricts paclitaxel delivery in approximately 90% of CNS lesions. Therefore to improve efficacy, we have developed an ultra-small hyaluronic acid (HA) nanoconjugate (∼5 kDa) can passively diffuse across leaky blood-tumor barrier and then be taken up into cancer cells...
A potential means of pharmacological management ischemic stroke is rapid intervention using potent neuroprotective agents. Thyroid hormone (T3) has been shown to protect against damage in middle cerebral artery occlusion (MCAO) model brain stroke. While thyroid permeable across the blood-brain barrier, we hypothesized that efficacy can be enhanced by encapsulation nanoparticulate delivery vehicles. We tested our hypothesis generating poly-(lactide-co-glycolide)-polyethyleneglycol...
Brain-targeted Tempol-loaded poly-(lactide-co-glycolide) (PLGA) nanoparticles (NPs) conjugated with a transferrin antibody (OX 26) were developed using the nanoprecipitation method. These NPs may have utility in treating neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Central to these is an increased production of reactive oxygen nitrogen species which take part development conditions. As proof principle, loaded Tempol, free radical scavenger that has been...
Introduction: Microdialysis is an important in vivo sampling technique, useful assaying extracellular tissue fluid. The technique has preclinical and clinical applications widely used neuroscience. In microdialysis allows measurement of neurotransmitters including acetylcholine, dopamine, norepinephrine 5-HT, amino acids (e.g., glutamate GABA), neurotransmitter metabolites neuropeptides fluid discrete brain regions laboratory animals rodents non-human primates.
Abstract Cilia are mechanosensing organelles that communicate extracellular signals into intracellular responses. Altered functions of primary cilia play a key role in the development various diseases including polycystic kidney disease. Here, we show endothelial cells from oak ridge (Tg737 orpk/orpk ) mouse, with impaired assembly, exhibit reduction actin stress fibers and focal adhesions compared to wild‐type (WT). In contrast, polycystin‐1 deficient mice (pkd1 null/null ), function,...
Mitochondrial metabolism plays an integral role in glucose-stimulated insulin secretion (GSIS) β-cells. In addition, the diabetogenic of glucagon released from α-cells a major etiology both type 1 and 2 diabetes because unopposed hyperglucagonemia is pertinent contributor to diabetic hyperglycemia. Titrating expression levels mitochondrial protein mitoNEET powerful approach fine-tune capacity cells. Mechanistically, β-cell-specific induction causes hyperglycemia glucose intolerance due...
Objectives: To prepare and characterize in vitro a novel brain-targeted delivery of doxorubicin using glutathione-coated nanoparticles (NPs) for the treatment brain cancer.Methods: Doxorubicin-loaded NPs were prepared by nanoprecipitation method PLGA-COOH (dl-lactide-co-glycolide). The coated with glutathione-PEG conjugate (PEG-GSH) order to target brain. characterized via studies determine particle size, drug release, cellular uptake, immunofluorescence study, cytotoxic assay, blood–brain...
Hepatocellular carcinoma (HCC), one of the most common cancers in world, is a leading cause cancerrelated mortality. HCC develops frequently background oxidative stress and chronic hepatic inflammation due to viral infections, alcohol abuse as well exposure environmental dietary carcinogens. As prognosis extremely poor mostly unresponsive current chemotherapeutic treatment regimens, novel preventive approaches like chemoprevention are urgently needed. We have recently found that resveratrol,...