A5039114979- Ion Channels and Receptors
- Cardiac Fibrosis and Remodeling
- Angiogenesis and VEGF in Cancer
- Thermoregulation and physiological responses
- Cardiovascular Function and Risk Factors
- Cell Adhesion Molecules Research
- Genetic and Kidney Cyst Diseases
- Inflammatory mediators and NSAID effects
- Cardiovascular Effects of Exercise
- Cardiac electrophysiology and arrhythmias
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Mast cells and histamine
- Bioactive Compounds and Antitumor Agents
- Orthopaedic implants and arthroplasty
- Tissue Engineering and Regenerative Medicine
- Cardiac Ischemia and Reperfusion
- NF-κB Signaling Pathways
- Peptidase Inhibition and Analysis
- Chemokine receptors and signaling
- Biomedical Research and Pathophysiology
- Fibroblast Growth Factor Research
- Retinal Diseases and Treatments
- Phytochemicals and Antioxidant Activities
- Free Radicals and Antioxidants
- Microtubule and mitosis dynamics
University of Toledo
2021-2024
Northeast Ohio Medical University
2013-2023
Kent State University
2014-2020
Integrative Medicine Institute
2015
Ohio University
2013
Medical College of Wisconsin
2012
University of Padua
2012
University of Michigan
2009-2010
Transient receptor potential vanilloid channel 4 (TRPV4) is a polymodally activated nonselective cationic implicated in the regulation of vasodilation and hypertension. We others have recently shown that cyclic stretch shear stress activate TRPV4-mediated calcium influx endothelial cells (EC). In addition to mechanical forces, acetylcholine (ACh) was cells, which important for nitric oxide-dependent vasodilation. However, molecular mechanism through ACh activates TRPV4 not known. Here, we...
We previously reported that type VI collagen deposition increases in the infarcted myocardium vivo. To date, a specific role for this nonfibrillar has not been explored setting of myocardial infarction (MI).To determine whether deletion an vivo model post-MI wound healing would alter cardiac function and remodeling days to weeks after injury.Wild-type Col6a1(-/-) mice were subjected MI, followed by serial echocardiographic histological assessments. At 8 infarct size was significantly...
// Roslin J. Thoppil 1, 2, * , Holly C. Cappelli Ravi K. Adapala 2 Anantha Kanugula 1 Sailaja Paruchuri 3 Charles Thodeti Department of Integrative Medical Sciences, Northeast Ohio University, OH 44272, Rootstown, USA School Biomedical Kent State 44240, Kent, Chemistry, University Akron, 44325, These authors contributed equally to this work Correspondence to: Thodeti, e-mail: cthodeti@neomed.edu Keywords: endothelial cell, mechanotransduction, Rho/Rho kinase, TRPV4, tumor angiogenesis...
Cysteinyl leukotrienes (cys-LTs), LTC4, LTD4, LTE4 are potent inflammatory lipid mediators that act through two distinct G-protein-coupled receptors, CysLT1R and CysLT2R. Although cys-LTs shown to induce vascular leakage atherosclerosis, the molecular mechanism by which modulate endothelial function is not known. Here, we show (LTC4 LTD4) robust calcium influx in human umbilical vein cells (HUVECs) CysLT2R, but CysLT1R. Further, cys-LT treatment induced cell (EC) contraction leading...
Abstract Cilia are mechanosensing organelles that communicate extracellular signals into intracellular responses. Altered functions of primary cilia play a key role in the development various diseases including polycystic kidney disease. Here, we show endothelial cells from oak ridge (Tg737 orpk/orpk ) mouse, with impaired assembly, exhibit reduction actin stress fibers and focal adhesions compared to wild‐type (WT). In contrast, polycystin‐1 deficient mice (pkd1 null/null ), function,...
Abstract Endothelial cell proliferation is a critical event during angiogenesis, regulated by both soluble factors and mechanical forces. Although the of tumor cells studied extensively, little known about endothelial (TEC) its contribution to angiogenesis. We have recently shown that reduced expression mechanosensitive ion channel TRPV4 in TEC causes aberrant mechanosensitivity result abnormal Here, we show display increased compared normal (NEC). Further, found exhibit high basal ERK1/2...
Significance Cysteinyl leukotrienes (cys-LTs) are proinflammatory mediators and regulate endothelial cell contraction, permeability, angiogenesis via a cys-LT receptor, CysLT 2 R. In response to the challenges of finding therapies that can combat tumor metastases, we explored advantage targeting this receptor. We show R mediates contributes metastasis in vivo independent VEGF by enhancing leakiness permeability blood vessels. propose antagonist, BayCysLT , normalize vessels, reducing growth...
Diabetes is an independent risk factor for cardiovascular disease that can eventually cause cardiomyopathy and heart failure. Cardiac fibroblasts (CF) are the critical mediators of physiological pathological cardiac remodeling; however, effects hyperglycemia on fibroblast function differentiation not well known. Here, we performed a comprehensive investigation show enhances differentiation. We found high glucose treatment increased collagen I, III, VI gene expression in rat adult...
Left ventricular hypertrophy is a bipolar response, starting as an adaptive response to the hemodynamic challenge, but over time develops maladaptive pathology partly due microvascular rarefaction and impaired coronary angiogenesis. Despite profound influence on cardiac function, mechanotransduction mechanisms that regulate angiogenesis, leading heart failure, are not well known.
Hyperleptinemia, characteristic of diabetes and a hallmark feature human obesity, contributes to the increased risk atherosclerotic complications. However, molecular mechanisms mediating leptin-induced atherogenesis gene expression in vascular cells remain incompletely understood. Accumulating evidence documents critical role potent antiangiogenic proatherogenic matricellular protein, thrombospondin-1 (TSP-1), atherosclerosis. Although previous studies reported elevated TSP-1 levels both...
ABSTRACT VEGF signaling via receptor‐2 (VEGFR2) is a major regulator of endothelial cell (EC) functions, including angiogenesis. Although most studies angiogenesis focus on soluble signaling, mechanical also plays critical role. Here, we examined the consequence disruption pathways. Specifically, observed that small interfering RNA (siRNA) knockdown mechanosensitive ion channel, transient receptor potential vanilloid 4 (TRPV4), significantly reduced perinuclear (Golgi) VEGFR2 in human ECs...
Tumor angiogenesis is initiated and maintained by the tumor microenvironment through secretion of autocrine paracrine factors, including extracellular vesicles (EVs). Although tumor-derived EVs (t-EVs) have been implicated in angiogenesis, growth metastasis, most studies on t-EVs are focused proangiogenic miRNAs factors. We recently demonstrated that conditioned media from human lung cells (A549) downregulate TRPV4 channels transform normal endothelial to a cell-like phenotype induce...
The soluble and mechanical microenvironment surrounding endothelial cells influences instructs them to form new blood vessels. in the pathological tumor release extracellular vesicles (EVs) for paracrine signaling. EVs have been shown induce angiogenesis by communicating with cells, but underlying molecular mechanisms are not well known. We recently that mechanosensitive ion channel transient receptor vanilloid 4 (TRPV4) expression activity is significantly reduced (TEC), activation of TRPV4...
Abstract Transient receptor potential vanilloid 4 (TRPV4) channels are mechanosensitive ion that regulate systemic endothelial cell (EC) functions such as vasodilation, permeability, and angiogenesis. TRPV4 is expressed in retinal ganglion cells, Müller glia, pigment epithelium, microvascular ECs, modulates volume regulation, calcium homeostasis, survival. TRPV4‐mediated physiological or pathological angiogenesis remains poorly understood. Here, we demonstrate expressed, functional, ECs. The...
Abstract Previously, we used 2D films to identify an annealed PCL‐PDLLA phase‐separated blend morphology which provided nanoscale surface texture and patterning that stimulated osteoblast differentiation. In order translate these nanopatterning effects the walls of 3D salt‐leached scaffolds, phase scaffold must be characterized. For NaCl is as a porogen, may affect separation in blends. However, it not possible characterize using standard approaches such AFM or optical microscopy, since...