A5006238724- Ion Channels and Receptors
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Mast cells and histamine
- Asthma and respiratory diseases
- Thermoregulation and physiological responses
- Angiogenesis and VEGF in Cancer
- Cardiac Fibrosis and Remodeling
- Inflammatory mediators and NSAID effects
- Cell Adhesion Molecules Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Crystallography and molecular interactions
- Cytokine Signaling Pathways and Interactions
- Fibroblast Growth Factor Research
- Bioactive Compounds and Antitumor Agents
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Cardiovascular Function and Risk Factors
- Cardiac electrophysiology and arrhythmias
- Luminescence and Fluorescent Materials
- Molecular Sensors and Ion Detection
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Protease and Inhibitor Mechanisms
- Chemokine receptors and signaling
University of Toledo
2021-2024
University of Akron
2013-2022
Northeast Ohio Medical University
2014-2016
Integrative Medicine Institute
2015
Cleveland Clinic
2014
iRobot (United States)
2014
Harvard University
2006-2011
Brigham and Women's Hospital
2006-2011
ENT and Allergy
2008-2009
Boston Children's Hospital
2006-2007
Idiopathic pulmonary fibrosis (IPF) is a fatal fibrotic lung disorder with no effective medical treatments available. The generation of myofibroblasts, which are critical for fibrogenesis, requires both mechanical signal and activated TGF-β; however, it not clear how fibroblasts sense transmit the signal(s) that promote differentiation into myofibroblasts. As transient receptor potential vanilloid 4 (TRPV4) channels in response to changes plasma membrane stretch/matrix stiffness, we...
Of the potent lipid inflammatory mediators comprising cysteinyl leukotrienes (LTs; LTC4, LTD4, and LTE4), only LTE4 is stable abundant in vivo. Although shows negligible activity at type 1 2 receptors for cys-LTs (CysLT1R CysLT2R), it a powerful inducer of mucosal eosinophilia airway hyperresponsiveness humans with asthma. We show that adenosine diphosphate (ADP)–reactive purinergic (P2Y12) receptor required LTE4-mediated pulmonary inflammation. P2Y12 expression permits -induced activation...
An NIR-emitting probe (λem ∼ 700 nm) with a large Stokes shift (Δλ ≈ 234 is synthesized by using excited-state intramolecular proton transfer (ESIPT). The phenolic proton, which controls ESIPT, acts as switch to give strong fluorescence at pH 5. can selectively show lysosome organelles, therefore leading without exhibiting "an alkalinizing effect".
In situ analysis of fetal semilunar valve leaflets has revealed cells coexpressing endothelial and mesenchymal markers along the endothelium, with diminished frequency seen in adult valves. To determine whether such are progenitor cells, we isolated clonal populations from human pulmonary The clones expressed but showed potential to further differentiate into endothelium response vascular growth factor (VEGF)-A. When exposed transforming (TGF)-β 2 , individual adopted a phenotype varying...
Cysteinyl leukotrienes (cys-LTs) are potent inflammatory lipid mediators, of which leukotriene (LT) E(4) is the most stable and abundant in vivo. Although only a weak agonist established G protein-coupled receptors (GPCRs) for cys-LTs, LTE(4) potentiates airway hyper-responsiveness (AHR) by cyclooxygenase (COX)-dependent mechanism induces bronchial eosinophilia. We now report that activates human mast cells (MCs) pathway involving cooperation between an MK571-sensitive GPCR peroxisome...
The fluorescence pattern of proteins in the polyacrylamide gel by using flavone dye staining without washing process.
// Roslin J. Thoppil 1, 2, * , Holly C. Cappelli Ravi K. Adapala 2 Anantha Kanugula 1 Sailaja Paruchuri 3 Charles Thodeti Department of Integrative Medical Sciences, Northeast Ohio University, OH 44272, Rootstown, USA School Biomedical Kent State 44240, Kent, Chemistry, University Akron, 44325, These authors contributed equally to this work Correspondence to: Thodeti, e-mail: cthodeti@neomed.edu Keywords: endothelial cell, mechanotransduction, Rho/Rho kinase, TRPV4, tumor angiogenesis...
Cysteinyl leukotrienes (cys-LTs), LTC4, LTD4, LTE4 are potent inflammatory lipid mediators that act through two distinct G-protein-coupled receptors, CysLT1R and CysLT2R. Although cys-LTs shown to induce vascular leakage atherosclerosis, the molecular mechanism by which modulate endothelial function is not known. Here, we show (LTC4 LTD4) robust calcium influx in human umbilical vein cells (HUVECs) CysLT2R, but CysLT1R. Further, cys-LT treatment induced cell (EC) contraction leading...
Abstract Endothelial cell proliferation is a critical event during angiogenesis, regulated by both soluble factors and mechanical forces. Although the of tumor cells studied extensively, little known about endothelial (TEC) its contribution to angiogenesis. We have recently shown that reduced expression mechanosensitive ion channel TRPV4 in TEC causes aberrant mechanosensitivity result abnormal Here, we show display increased compared normal (NEC). Further, found exhibit high basal ERK1/2...
Significance Cysteinyl leukotrienes (cys-LTs) are proinflammatory mediators and regulate endothelial cell contraction, permeability, angiogenesis via a cys-LT receptor, CysLT 2 R. In response to the challenges of finding therapies that can combat tumor metastases, we explored advantage targeting this receptor. We show R mediates contributes metastasis in vivo independent VEGF by enhancing leakiness permeability blood vessels. propose antagonist, BayCysLT , normalize vessels, reducing growth...
We have recently shown that leukotriene D4 (LTD4)increases cell survival in intestinal epithelial cells. Here we report and explore the complementary finding LTD4 also enhances proliferation these This proliferative response was approximately half of induced by epidermal growth factor (EGF) its required activation protein kinase C (PKC), Ras mitogen-activated (MAPK) Erk-1/2. EGF activated Erk-1/2 cells; however EGF-receptor inhibitor PD153035 did not affect LTD4-induced In addition, induce...
A high throughput screening technology enables identifying natural compounds, phytochemicals, that potently inhibit migration of metastatic breast cancer cells.
A rational design has led to a highly selective and cell-permeable zinc sensor, which exhibits not only large fluorescence turn-on at ~545 nm but also the desirable NIR emission (~720 nm) with Stokes' shift, providing practical sensor platform two channels for reliable detection.
Left ventricular hypertrophy is a bipolar response, starting as an adaptive response to the hemodynamic challenge, but over time develops maladaptive pathology partly due microvascular rarefaction and impaired coronary angiogenesis. Despite profound influence on cardiac function, mechanotransduction mechanisms that regulate angiogenesis, leading heart failure, are not well known.