A5066617857- Viral Infections and Outbreaks Research
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Vectors
- Immune Cell Function and Interaction
- Animal Disease Management and Epidemiology
- RNA Interference and Gene Delivery
- Vector-Borne Animal Diseases
- Chronic Lymphocytic Leukemia Research
- Clinical Nutrition and Gastroenterology
- Respiratory viral infections research
- T-cell and Retrovirus Studies
- Cancer-related Molecular Pathways
- Cell Image Analysis Techniques
- bioluminescence and chemiluminescence research
- T-cell and B-cell Immunology
- Retinal Development and Disorders
- Poisoning and overdose treatments
- Cardiac tumors and thrombi
- Cancer, Stress, Anesthesia, and Immune Response
- Genetic Syndromes and Imprinting
- Mosquito-borne diseases and control
- Cancer-related molecular mechanisms research
- Nitric Oxide and Endothelin Effects
- Pneumonia and Respiratory Infections
- Cellular Mechanics and Interactions
Copenhagen University Hospital
2024
University of Copenhagen
2024
National Institute of Allergy and Infectious Diseases
2014-2023
Aarhus University
1936-2023
United States Army Medical Research Institute of Infectious Diseases
2016-2020
National Institutes of Health
1992-2015
National Cancer Institute
1996-2013
Center for Cancer Research
2013
Frederick National Laboratory for Cancer Research
2011
Vrije Universiteit Amsterdam
1992
Paradoxical tuberculosis-associated immune reconstitution inflammatory syndrome (TB-IRIS) is an aberrant response occurring in a subset of TB-HIV co-infected patients initiating anti-retroviral therapy (ART). Here, we examined monocyte activation by prospectively quantitating pro-inflammatory plasma markers and subsets from South Indian cohort at baseline following ART initiation the time IRIS, or equivalent points non-IRIS controls. Pro-inflammatory biomarkers innate myeloid cell were...
A detailed understanding of serological immune responses to Ebola and Marburg virus infections will facilitate the development effective diagnostic methods, therapeutics, vaccines. We examined antibodies from or survivors 1 14 years after recovery disease, by using a microarray that displayed recombinant nucleoprotein (NP), viral protein 40 (VP40), envelope glycoprotein (GP), inactivated whole virions six species filoviruses. All three outbreak cohorts exhibited significant antibody antigens...
Zika virus (ZIKV) infections occur in areas where dengue (DENV), West Nile (WNV), yellow fever (YFV), and other viruses of the genus Flavivirus cocirculate. The envelope (E) proteins these closely related flaviviruses induce specific long-term immunity, yet subsequent are associated with cross-reactive antibody responses that may enhance disease susceptibility severity. To gain a better understanding ZIKV against background similar viral diseases, we examined serological immune to ZIKV, WNV,...
We have developed a digital and multiplexed platform for the rapid detection telemonitoring of infections caused by Ebola Marburg filoviruses. The system includes flow cell assay cartridge that captures specific antibodies with microarrayed recombinant antigens from all six species filovirus, smartphone fluorescent reader high-performance interpretation test results. Multiplexed viral proteins, which are expandable to include greater numbers probes, were incorporated obtain highest...
Nuclear primary microRNA (pri-miRNA) processing catalyzed by the DGCR8-Drosha (Microprocessor) complex is highly regulated. Little known, however, about how biogenesis spatially organized within mammalian nucleus. Here, we image for first time, in living cells and at level of a single cluster, intra-nuclear distribution untagged, endogenously-expressed pri-miRNAs generated human imprinted Chromosome 19 MicroRNA Cluster (C19MC), from environment transcription sites to molecules fully released...
An accurate understanding of the physicochemical properties bimetallic heterogeneous catalysts relies on atomic-scale knowledge surface morphology and atomic distribution. Alloys Cu Zn created during catalyst operation are frequently studied debated in relation to a description active phase Cu/ZnO/Al2O3 methanol synthesis catalysts. This makes it relevant build better dissolution pathways surfaces resulting morphology. Herein, we use scanning tunneling microscopy investigate distinct atom...
The antiviral lectins griffithsin (GRFT), cyanovirin-N (CV-N), and scytovirin (SVN), which inhibit several enveloped viruses, including lentiviruses, were examined for their ability to entry mediated by Env proteins of delta- gammaretroviruses. glycoproteins from human T-cell leukemia virus type 1 (HTLV-1) resistant the effects all three lectins. For gammaretroviruses, CV-N inhibited some but not envelopes examined, whereas GRFT SVN displayed only little or no effect.
Ongoing research efforts into fluorescent proteins continuously generates new mutation variants, some of which can become photoactivated or photoconverted to a red-shifted color upon intense UV blue light illumination. We report built-in propensity for enhanced yellow protein (EYFP) undergo irreversible photoconversion cyan (CFP)-like species green-light The is thermally activated, happens mainly in fixed, nonsealed cell samples, and may result very bright relatively photostable CFP-like...
Objective: Evaluate baseline differences by HIV status and the impact of pomalidomide on lymphocyte counts T cell subsets in patients with Kaposi sarcoma (KS). Design: We prospectively evaluated CD4 + CD8 phenotypes 19 participants KS enrolled a phase 1/2 study (NCT01495598), 7 without 12 antiretroviral therapy. Methods: Trial received 5 mg orally for 21 days 28-day cycles up to 1 year. Flow cytometry was performed peripheral blood mononuclear cells at baseline, after 3 cycles,...
Gene silencing by RNA interference (RNAi) can be achieved the ectopic expression of tailored short hairpin RNAs (shRNAs) which after export to cytoplasm are processed Dicer and incorporated into induced complex (RISC). Design rules for shRNAs have been focus several studies, but only a few reports turned attention sequence loop-region. In this work we selected high-functional low-functional shRNA loops from retroviral hairpin-loop-libraries in an RNAi reporter assay. The procedure revealed...
Early studies mapped the entry receptor for HTLV-1 and HTLV-2 to long arm of chromosome 17. However, later observations that titer HTLV Env-pseudotyped viruses was low on some mouse-human hybrid cell lines containing human 17q led belief this gene not involved in infection. Recent showing efficient HTLV-I infection requires three molecules (GLUT-1, NRP-1, HSPGs), prompted us examine whether a 17 is related complex. Since NRP-1 or GLUT-1 map other chromosomes, we examined genes synthesis...
Background: The aim of the study is to develop a low cost system for studying antibody responses Ebola and Marburg infection surveillance purposes. There are no serological assays that available in common clinical practice detection filovirus responses. Most experimental methods capable only measuring antibodies against one or two five species viruses cause human disease. developed included flow cell assay cartridge captures specific with microarrayed recombinant antigens from all six...