A5064329235- Reproductive Biology and Fertility
- Tissue Engineering and Regenerative Medicine
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Reproductive Physiology in Livestock
- Mesenchymal stem cell research
- Kruppel-like factors research
- Molecular Biology Techniques and Applications
- Reproductive System and Pregnancy
- RNA Interference and Gene Delivery
- TGF-β signaling in diseases
- Estrogen and related hormone effects
- NF-κB Signaling Pathways
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Genetic Mapping and Diversity in Plants and Animals
- Bone Metabolism and Diseases
- Sirtuins and Resveratrol in Medicine
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Ovarian function and disorders
- Cancer-related molecular mechanisms research
- Liver physiology and pathology
- Circular RNAs in diseases
Wroclaw Medical University
2023-2024
Poznan University of Medical Sciences
2007-2023
Heliodor Swiecicki Clinical Hospital
2016-2020
University of Life Sciences in Poznań
2016
Research Institute for Farm Animal Biology (FBN)
2011
Epithelial-mesenchymal transition (EMT) is a crucial process with significance in the metastasis of malignant tumors. It through acquisition plasticity that cancer cells become more mobile and gain ability to metastasize other tissues. The mesenchymal-epithelial (MET) return an epithelial state, which allows for formation secondary Both processes, EMT MET, are regulated by different pathways mediators, affects sophistication overall tumorigenesis process. Not insignificant also stem their...
Abstract Heart failure (HF) is an end-stage of many cardiac diseases and one the main causes death worldwide. The current management this disease remains suboptimal. adult mammalian heart was considered a post-mitotic organ. However, several reports suggest that it may possess modest regenerative potential. Adult progenitor cells (CPCs), players in regeneration, constitute, as seem, heterogenous group cells, which remain quiescent physiological conditions become activated after injury,...
Proper course of folliculogenesis and oogenesis have an enormous impact on female fertility. Both processes take place in the ovary involve not only maturing germ cell, but also few types somatic cells that assist ovarian mediate dialog with oocyte. These cells, granulosa theca, are heavily involved essential reproductive processes, such as ovulation, fertilization, embryo implantation. In this study, we used expressive microarray approach to analyze transcriptome porcine during short-term...
Photobiomodulation (PBM), also called low-level laser treatment (LLLT), has been considered a promising tool in periodontal due to its anti-inflammatory and wound healing properties. However, photobiomodulation’s effectiveness depends on combination of parameters, such as energy density, the duration frequency irradiation sessions, wavelength, which shown play key role laser-tissue interaction. The objective study was compare vitro effects two different wavelengths—635 nm 808 nm—on human...
The human ovarian granulosa cells (GCs) surround the oocyte and form proper architecture of follicle. ability GCs to proliferate differentiate in conditions vitro culture has been proven. However, there is still a large field for extensive investigation molecular basics, as well marker genes, responsible these processes. This study aimed find new encoding proteins that regulate capability proliferation differentiation during long-term primary culture. follicular were collected from...
The growth and development of oocyte affect the functional activities surrounding somatic cells. These cells are regulated by various types hormones, proteins, metabolites, regulatory molecules through gap communication, ultimately leading to maturation oocytes. close association between oocytes, which together form cumulus-oocyte complexes (COCs), their bi-directional communication crucial for acquisition developmental competences oocyte. In this study, oocytes were extracted from ovaries...
Spermatid-specific linker histone H1-like protein (HILS1), transition proteins 1 and 2 (TNP1 TNP2), protamines (PRM1 PRM2) contribute to considerable dense packing of spermatid chromatin during spermiogenesis. We evaluated the HILS1, TNP1, TNP2 transcript levels in spermatozoa isolated from normozoospermic asthenozoospermic men. Human ejaculates (n = 70) 100) donors were purified by centrifugation through a discontinuous Percoll density gradient. RNA was according Chomczyński Sacchi method,...
The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation transformation granulosa cells (GCs) during folliculogenesis, oogenesis, oocyte maturation. Although role 17 beta-estradiol (E2) ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, GCs-luteal requires further research. Therefore, goal this study was to assess real-time porcine GCs vitro relation...
Mammalian oocyte maturation is achieved when oocytes reach metaphase II (MII) stage, and accumulate mRNA proteins in the cytoplasm following fertilization. It has been shown that investigated before after vitro (IVM) differ significantly transcriptomic proteomic profiles. Additionally, folliculogenesis oogenesis accompanied by morphogenetic changes, which influence further zygote formation embryo growth. This study aimed to determine new markers of porcine morphogenesis are associated with...
Maturation of cumulus-oocyte complexes (COCs) is crucial for further successful monospermic fertilization, embryo growth, and implantation. All these events are accompanied by proliferation differentiation cumulus cells. The migration COCs to the oviduct after ovulation interaction between female gametes and/or embryos with maternal tissues still poorly recognized on molecular level. This study was aimed first demonstrate mRNA expression profile cell markers during different stages porcine...
Proper oocyte maturation in mammals produces an capable of monospermic fertilization and embryo preimplantation. The cumulus-oocyte complexes (COCs), surrounding oocyte, play a significant role maturation. During this process, when the COCs undergo cumulus expansion wherein tightly compact cells (CCs) form dispersed structure, permanent biochemical molecular modifications occur maturing oocytes, indicating that gene expression between immature mature oocytes differs significantly. This study...
Steroid hormone secretion is one of the key functions granulosa cells (GCs). Resveratrol a natural polyphenol, known for its beneficial health effects, such as improving reproductive health. However, application limited due to poor bioavailability. The methoxy derivative resveratrol (DMU-212) was demonstrated be more lipophilic, and therefore greater since addition groups stilbene scaffold found make molecule insoluble in water, DMU-212 loaded into liposomes. This study aimed evaluate how...
Summary The oocyte growth and development in follicular environment are substantially accompanied by surrounding somatic cumulus (CCs) granulosa cells (GCs). During these processes, the mammalian gametes reach full maturational stage may be further successfully fertilized single spermatozoon. These unique mechanisms regulated expression of clusters genes their biochemical signaling pathways. In this article we described differential pattern transforming factor beta (TGFB) gene superfamily...
The full maturational capability of mammalian oocytes is accompanied by nuclear and cytoplasmic modifications, which are associated with proliferation differentiation surrounding cumulus cells. These events regulated on molecular level the expression target genes involved in signal transduction pathways crucial for folliculogenesis oogenesis. Transforming growth factor beta signaling includes several molecules that regulation oogenesis embryo growth, including bone morphogenetic protein...
Abstract The ovary is part of the reproductive system, possessing very important functions in reproduction process (ovum and embryo transfer, providing a suitable environment for sperm capacitation, etc.). There are two types cells fallopian tubes: alveolar secretive cells. These study shows metabolic processes pig oviductal epithelial associated with activation signaling pathways amino acids metabolism degradation during long-term vitro culture. Oviductal from 45 colonies anestrous phase...
Abstract The efficiency of the process obtaining mature oocytes, and then porcine embryos in vitro depends on many factors requires meeting conditions. These include selection morphologically appropriate medium components, as well a number abiotic (appropriate microenvironment during culture). Oocytes were taken from 45 pubertal crossbred Landrace gilts. BCB test was carried out. + oocytes divided into two groups: “before IVM” “after IVM”. “Before subjected to molecular analyzes immediately...
Abstract The morphological and biochemical modification of oviductal epithelial cells (OECs) belongs to the group compound processes responsible for proper oocyte transport successful fertilization. cellular interactions between cumulus-oocyte complexes (COCs) are crucial this unique mechanism. In present study we have analyzed angiogenesis blood vessel development at transcript levels. By employing microarrays, four ontological groups associated with these mechanisms been described....