A5086301153- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Genomics and Phylogenetic Studies
- Insect-Plant Interactions and Control
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Forensic Entomology and Diptera Studies
- Chromium effects and bioremediation
- Dermatological diseases and infestations
- Identification and Quantification in Food
- Evolution and Genetic Dynamics
- Cephalopods and Marine Biology
- Spectroscopy and Chemometric Analyses
- bioluminescence and chemiluminescence research
- Plant Virus Research Studies
- Adenosine and Purinergic Signaling
- Advanced Chemical Sensor Technologies
- Entomopathogenic Microorganisms in Pest Control
- Plant and Fungal Interactions Research
- Ion Channels and Receptors
- Nematode management and characterization studies
University of Liverpool
2014-2024
University of Leeds
2010-2024
University of York
2017
Bangor University
2009
Microbes can defend their host against virulent infections, but direct evidence for the adaptive origin of microbe-mediated protection is lacking. Using experimental evolution a novel, tripartite interaction, we demonstrate that mildly pathogenic bacteria (Enterococcus faecalis) living in worms (Caenorhabditis elegans) rapidly evolved to animal hosts infection by more pathogen (Staphylococcus aureus), crossing parasitism-mutualism continuum. Host all six, independently selected populations...
Maternally heritable symbionts are common in arthropods and represent important partners antagonists. A major impediment to understanding the mechanistic basis of these symbioses has been lack genetic manipulation tools, for instance, those enabling transgenic GFP expression systems vivo visualization. Here, we transform 'son-killer' reproductive parasite Arsenophonus nasoniae that infects parasitic wasp Nasonia vitripennis with plasmid pOM1-gfp, re-introduce this strain N. then used system...
Although the intracellular bacterium Wolbachia is ubiquitous in insects, it has a unique relationship with New World ants on which particular bacterial strains have specialized. However, data are from distantly related hosts and detailed phylogenetic information could reveal transmission dynamics lacking. Here, we investigate host-Wolbachia relationships monophyletic fungus-growing ant tribe Attini, screening 23 species using multilocus sequence typing to reliably identify strains. This...
Symbionts can substantially affect the evolution and ecology of their hosts. The investigation tissue-specific distribution symbionts (tissue tropism) provide important insight into host-symbiont interactions. Among other things, it help to discern importance specific transmission routes potential phenotypic effects. intracellular bacterial symbiont Wolbachia has been described as greatest ever panzootic, due wide array arthropods that infects. Being primarily vertically transmitted, is...
A dynamic continuum exists from free-living environmental microbes to strict host-associated symbionts that are vertically inherited. However, knowledge of the forces drive transitions in symbiotic lifestyle and transmission mode is lacking. Arsenophonus a diverse clade bacterial symbionts, comprising reproductive parasites coevolving obligate mutualists, which predominant vertical. We describe symbiosis between member genus Western honey bee. The symbiont shares common genomic predicted...
Heritable microbial symbionts have profound impacts upon the biology of their arthropod hosts. Whilst our current understanding dynamics these is typically cast within a framework vertical transmission only, horizontal has been observed in number cases. For instance, several can transmit horizontally when parasitoid hosts share oviposition patches with uninfected conspecifics, phenomenon called superparasitism. Despite this, transmission, and host contact structures that facilitates it, not...
The genus Arsenophonus has been traditionally considered to comprise heritable bacterial symbionts of arthropods. Recent work reported a microbe related the type species nasoniae as infecting honey bee, Apis mellifera. association was unusual for members in that microbe-host interaction arose through environmental and social exposure rather than vertical transmission. In this study, we describe vitro culture ArsBeeUST, strain isolated from A. mellifera USA. 16S rRNA sequence indicates it...
Resident microbes (microbiota) can shape host organismal function and adaptation in the face of environmental change. Invasion new habitats exposes hosts to novel selection pressures, but little is known about impact on microbiota host-microbiome relationship (e.g., how rapidly microbial associations are formed, whether influence invasion success). We used high-throughput 16S rRNA sequencing New Zealand (native) European (invasive) populations freshwater snail Potamopyrgus antipodarum found...
The biology of many bacteria is critically dependent on genes carried plasmid and phage mobile elements. These elements shuttle between microbial species, thus providing an important source biological innovation across taxa. It has recently been recognized that are also in symbiotic bacteria, which form long-lasting interactions with their host. In this study, we report a bacterial symbiont genome carries highly complex array these Arsenophonus nasoniae the son-killer microbe parasitic wasp...
// Amy E. Thurber 1, 2 , Michaela Nelson 3 Crystal L. Frost Michael Levin 4 William J. Brackenbury and David Kaplan 1 Program in Cell, Molecular, Developmental Biology, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, Massachusetts, USA Department Engineering, Medford, University York, Heslington, UK Biology Department, Center for Regenerative Correspondence to: Brackenbury, email: william.brackenbury@york.ac.uk Kaplan, david.kaplan@tufts.edu Keywords: oncochannel,...
Heritable microbes that exhibit reproductive parasitism are common in insects. One class of these the male-killing bacteria, which found a broad range insect hosts. Commonly, our knowledge incidence is based on one or few sampling sites, and degree causes spatial variation unclear. In this paper, we examine son-killer microbe Arsenophonus nasoniae across European populations its wasp host, Nasonia vitripennis. preliminary work, noticed two female N. vitripennis producing highly biased sex...
Abstract Body size and morphology are key fitness‐determining traits that can vary genotypically. They likely to be important in social insect queens, which mate swarms found colonies independently, but genetic influences on queen have been little investigated. Here, we show the body of queens influenced by their genotype leaf‐cutting ant Acromyrmex echinatior , a species certain lineages (patrilines) bias development towards reproductive rather than sterile workers. We no relationship...
Summary Endosymbiosis – where a microbe lives and replicates within host is an important contributor to organismal function that has accelerated evolutionary innovations catalysed the evolution of complex life. The processes associated with transitions endosymbiosis, however, are poorly understood. Here, we use comparative genomics genus Arsenophonus reveal occur on endosymbiotic lifestyle. We compared genomes 38 strains spanning diverse lifestyles from environmentally acquired infections...
The fitness effects of overt parasites, and host resistance to them, are well documented. Most symbionts, however, more covert their interactions with hosts less understood. Wolbachia , an intracellular symbiont insects, is particularly interesting because it thought be unaffected by the immune response have mostly focussed on sex ratio manipulation. Here, we use quantitative PCR investigate whether genotype affects infection density in leaf-cutting ant Acromyrmex echinatior may affect...
Endosymbiosis-where a microbe lives and replicates within host-is an important contributor to organismal function that has accelerated evolutionary innovations catalyzed the evolution of complex life. The processes associated with transitions endosymbiosis, however, are poorly understood. Here, we leverage wide diversity host-associated lifestyles genus Arsenophonus reveal occur during transition vertically transmitted endosymbiotic lifestyle from strains maintained solely by horizontal...
ABSTRACT The genus Arsenophonus has been traditionally considered to be comprised of heritable bacterial symbionts arthropods. Recent work reported a microbe related the type species A. nasoniae as infecting honey bee, Apis mellifera . association was unusual for members in that microbe-host interaction arose through environmental and social exposure rather than vertical transmission. In this study, we describe vitro culture ArsBeeUS, strain isolated from USA. 16S rRNA sequence clearly...
Heritable microbes that exhibit reproductive parasitism are common in insects. One class of these the male-killing bacteria, which found a broad range insect hosts. Commonly, our knowledge incidence is based on one or few sampling sites, and degree causes spatial variation unclear. In this paper, we examine son-killer microbe Arsenophonus nasoniae across European populations its wasp host, Nasonia vitripennis. preliminary work, noticed two female N. vitripennis producing highly biased sex...
Abstract A dynamic continuum exists from free-living environmental microbes to strict host associated symbionts that are vertically inherited. However, knowledge of the forces drive transitions in modes by which symbioses form is lacking. Arsenophonus a diverse clade bacterial symbionts, comprising reproductive parasites coevolving obligate mutualists, predominant mode transmission vertical. We describe symbiosis between member genus and Western honey bee. then present multiple lines...