A5024010518- Neonatal Respiratory Health Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Tracheal and airway disorders
- Congenital Diaphragmatic Hernia Studies
- Inhalation and Respiratory Drug Delivery
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cystic Fibrosis Research Advances
- ATP Synthase and ATPases Research
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Genetics, Bioinformatics, and Biomedical Research
- Bacillus and Francisella bacterial research
- Smoking Behavior and Cessation
- Drug Transport and Resistance Mechanisms
- RNA modifications and cancer
- Renal and related cancers
- Protist diversity and phylogeny
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- interferon and immune responses
- Peroxisome Proliferator-Activated Receptors
- Radioactivity and Radon Measurements
- Eosinophilic Disorders and Syndromes
- Cancer-related Molecular Pathways
- Pediatric health and respiratory diseases
- Marine Bivalve and Aquaculture Studies
University of Bern
2021-2023
University of Geneva
2017-2022
University Hospital of Geneva
2019-2021
Children's Hospital
2017
École Polytechnique Fédérale de Lausanne
2012-2014
Mediterranean Institute for Life Sciences
2009
Rudjer Boskovic Institute
2009
Abstract Background Congenital Pulmonary Airway Malformation (CPAM) has an estimated prevalence between 0.87 and 1.02/10,000 live births little is know about their pathogenesis. To improve our knowledge on these rare malformations, we analyzed the cellular origin of two most frequent CPAM, CPAM types 1 2, compared malformations with adjacent healthy lung human fetal lungs. Methods We prospectively enrolled 21 infants undergoing surgical resection for CPAM. Human samples were collected after...
Mouse lung developmental maturation and final alveolarization phase begin at birth. During this dynamic process, alveolar cells modify their morphology anchorage to the extracellular matrix. In particular, epithelial cell (AEC) type I undergo cytoplasmic flattening folding ensure alveoli lining. We developed FACS conditions for simultaneous isolation of endothelial in absence specific reporters during early middle phase. evidenced first time a pool extractable populations expressing high...
(1) Background: Stereological estimations significantly contributed to our anatomical and physiological understanding of the lung by providing an average distribution measured parameters over entire parenchyma. However, most structural diseases show a very inhomogeneous pattern alterations. (2) Methods: We developed novel protocol for analysis all pulmonary airspaces. Our pipeline starts with high-resolution synchrotron radiation-based X-ray tomographic microscopy (SRXTM) consists (i) image...
(1) Background: Stereological estimations significantly contributed to our understanding of lung anatomy and physiology. Taking stereology fully 3-dimensional facilitates the estimation novel parameters. (2) Methods: We developed a protocol for analysis all airspaces an entire lung. It includes (i) high-resolution synchrotron radiation-based X-ray tomographic microscopy, (ii) image segmentation using free machine-learning tool Ilastik ImageJ, (iii) calculation airspace diameter distribution...
Harmful consequences of cigarette smoke (CS) exposure during lung development can already manifest in infancy. In particular, early life to nicotine, the main component CS, was shown affect animal models. We aimed characterize effect nicotine on alveoli formation. analyzed kinetics normal alveolar alveolarization phase and then looked at a mouse model gestational exposure. Immunohistochemical staining revealed that wave cell proliferation [i.e., vascular endothelial cells, epithelial cells...
Chronic obstructive pulmonary disease (COPD) is among the top 5 causes of mortality in world and can develop as a consequence genetic and/or environmental factors. Current efforts are focused on identifying early life insults how these contribute to COPD development. In line with this, our study focuses influence nicotine exposure its potential impact (a) lung functions, (b) elastase-induced emphysema adulthood. To address this hypothesis, we developed model 2 hits, delivered at different...
Idiopathic pulmonary fibrosis (IPF) is a noninflammatory progressive lung disease. Oxidative damage hallmark of IPF, but the sources and consequences oxidant generation in lungs are unclear. In this study, we addressed link between H2O2-generating enzyme NADPH oxidase 4 (NOX4) di-tyrosine (DT), an oxidative post-translational modification IPF lungs. We performed immunohistochemical staining for DT NOX4 tissue from patients with controls using validated antibodies. healthy lung, showed little...
Keywords: CMG2 ; Anthrax Toxin Receptor palmitoylation ubiquitination These Ecole polytechnique federale de Lausanne EPFL, n° 6027 (2013)Programme doctoral Approches moleculaires du vivantFaculte des sciences la vieInstitut recherche en infectiologieUnite Prof. Gisou van der GootJury: B. Lemaitre (president), F.G. Goot Grunberg (directrice), Dr L. Chamberlain, V. Hatzimanikatis, H. Riezman (rapporteurs) Public defense: 2014-1-17 Reference doi:10.5075/epfl-thesis-6027Print copy in library...
Cystic fibrosis (CF) is characterized by thick airway mucus obstruction and enlarged small airways soon after birth. We hypothesized that CF might also influence early post-natal development of the conducting (CA). aimed to compare airspace diameters CA in βENaC-transgenic (tg) mice with CF-like lung disease control littermates at different stages (postnatal day (pnd) 3, 10, 21 36), applying synchrotron radiation-based X-ray tomographic microscopy (TOMCAT, SLS, Paul Scherrer Institute,...
Stereological estimations significantly contributed to our anatomical and physiological understanding of the lung. Typically, these represent an average over entire lung parenchyma. However, most structural diseases show a very inhomogeneous pattern alterations. Therefore, we developed novel protocol where all pulmonary airspaces are analyzed, regional differences easily detected. Our pipeline starts with high-resolution synchrotron radiation x-ray tomographic microscopy (SRXTM) consists (i)...
Genetic ion transport defects, like cystic fibrosis (CF), are known to cause chronic obstructive lung disease. However, it is not whether those defects could affect early development. To answer this question, we estimated quantitatively the 3D-lung structure and studied airway architecture of neonatal mice with CF‐like Lungs βENaC‐Tg control littermates were obtained at day 3, inflated 20 cm water column, critical point dried, scanned by synchrotron radiation-based X-ray tomographic...