A5011247798- Neonatal Respiratory Health Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory Support and Mechanisms
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Neuroscience of respiration and sleep
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pediatric health and respiratory diseases
- Congenital Diaphragmatic Hernia Studies
- Respiratory and Cough-Related Research
- Transplantation: Methods and Outcomes
- Obstructive Sleep Apnea Research
- Nitric Oxide and Endothelin Effects
- Inhalation and Respiratory Drug Delivery
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Smoking Behavior and Cessation
- Medical Imaging and Pathology Studies
- Tracheal and airway disorders
- Clinical Nutrition and Gastroenterology
- Phonocardiography and Auscultation Techniques
- Child and Adolescent Health
- Liver Disease and Transplantation
- Immune Response and Inflammation
- Adolescent and Pediatric Healthcare
- Asthma and respiratory diseases
University of Geneva
2009-2023
University Hospital of Geneva
2006-2021
Hôpital Beau-Séjour
2017-2019
Geneva College
2017
Children's Hospital
2006-2017
Boston Children's Hospital
2004
Pediatrics and Genetics
2001-2003
The pathogenesis of pulmonary fibrosis is linked to oxidative stress, possibly generated by the reactive oxygen species (ROS) generating NADPH oxidase NOX4. Epithelial cell death a crucial early step in development disease, followed only later fibrotic stage. We demonstrate that lungs patients with idiopathic lung fibrosis, there strong expression NOX4 hyperplastic alveolar type II cells.To study possible causative role cells, we have NOX4-deficient mice.Three weeks after administration...
Hyperoxia-induced lung damage was investigated via airway and respiratory tissue mechanics measurements with low-frequency forced oscillations (LFOT) analysis of spontaneous breathing indexes by barometric whole body plethysmography (WBP). WBP performed in the unrestrained awake mice kept room air ( n = 12) or 100% oxygen for 24 9), 48 8), 60 9) h, indexes, including enhanced pause (Penh) peak inspiratory expiratory flows, were determined. The then anesthetized, paralyzed, mechanically...
Reactive oxygen species (ROS) participate in the pathogenesis of emphysema. Among ROS-producing enzymes, NOX NADPH oxidases are thought to be responsible for tissue injury associated with several lung pathologies. To determine whether NOX2 and/or NOX1 development emphysema, their expression patterns were first studied by immunohistochemistry lungs emphysematous patients. Subsequently, we investigated contribution elastase-induced emphysema using NOX2- and NOX1-deficient mice. In human lung,...
Lung auscultation is fundamental to the clinical diagnosis of respiratory disease. However, a subjective practice and interpretations vary widely between users. The digitization acquisition interpretation particularly promising strategy for diagnosing monitoring infectious diseases such as Coronavirus-19 disease (COVID-19) where automated analyses could help decentralise care better inform decision-making in telemedicine. This protocol describes standardised collection lung auscultations...
Abstract Background Interstitial lung diseases (ILD), such as idiopathic pulmonary fibrosis (IPF) and non-specific interstitial pneumonia (NSIP), chronic obstructive disease (COPD) are severe, progressive disorders with a poor prognosis. Prompt accurate diagnosis is important to enable patients receive appropriate care at the earliest possible stage delay progression prolong survival. Artificial intelligence-assisted auscultation ultrasound (LUS) could constitute an alternative conventional,...
Idiopathic pulmonary fibrosis (IPF) is a severe progressive and irreversible lung disease. Novel antifibrotic drugs that slow disease progression are now available. However, many issues regarding patient management remain unanswered, such as the choice between available drugs, their use in particular subgroups clinical situations, time of treatment onset, termination, combination or switch, nonpharmacologic management. To guide Swiss respiratory physicians this evolving field still...
The latest guideline from the American Academy of Pediatrics for management bronchiolitis has helped reduce unnecessary interventions and costs. However, data on patients still receiving are missing. In with acute whose was assessed compared current achievable benchmarks care, we aimed to identify factors associated nonadherence recommendations. this single-centre retrospective study pre-guideline (Period 1: 2010 2012) two periods post-guideline 2: 2015 2016, early post-guideline; Period 3:...
Introduction Rigid bronchoscopy was traditionally performed in the management of foreign-body aspiration (FBA). More recently, since development a less invasive method, flexible has been proposed some centers for FBA. For past few years, we have applied decisional algorithm, privileging diagnosis and, cases, extraction foreign body (FB). Our aims are first to analyze our current FBA and second examine bronchoscopic findings complications. Materials Methods Retrospective medical chart review...
Background Pectus excavatum (PE) and pectus carinatum (PC) have generally been considered an aesthetic issue, although there is growing evidence of associated cardiopulmonary function (CPF) impairment, especially in PE patients. The study goal was to determine any correlation between malformations symptoms based on systematic assessment CPF thoracic measurements, such as Haller Index (HI) sternal torsion angle (STA). Methods Data from 76 adolescent patients with (n=30) or PC (n=46) were...
Objective: To report early blood exchange transfusion in malignant pertussis and a favorable clinical outcome. Setting: A pediatric intensive care unit tertiary hospital Geneva, Switzerland. Design: descriptive case report. Patient: An 8-wk-old girl was diagnosed with (extreme leukocytosis, seizures, pneumonia, secondary severe hypoxic respiratory failure associated pulmonary hypertension). After administration of one-volume transfusion, rapid decrease white cell count (from 119,000/mm3 to...
Leptin, a cytokine involved in the regulation of food intake, has been reported to be decreased lung diseases such as chronic obstructive pulmonary disease and cystic fibrosis increased critically ill patients with sepsis. We investigated role leptin during hyperoxia mice, which results alveolar edema, severe weight loss, death within 3–4 days. In oxygen-breathing serum was six- sevenfold its mRNA upregulated white adipose tissue. Leptin elevation could not attributed changes circulating...
Idiopathic pulmonary fibrosis (IPF) is a rapid progressive fibro-proliferative disorder with poor prognosis similar to lung cancer. The pathogenesis of IPF uncertain, but loss epithelial cells and fibroblast proliferation are thought be central processes. Previous reports have shown that BARD1 expression upregulated in response hypoxia associated TGF-β signaling, both recognized factors driving fibrosis. Differentially spliced isoforms, particular BARD1β, oncogenic drivers cancers various...
The uncoupling protein‐3 (UCP3) is a mitochondrial protein expressed mainly in skeletal muscle. Among several hypotheses for its physiological function, UCP3 has been proposed to prevent excessive production of reactive oxygen species. In the present study, we evaluated effect an oxidative stress induced by hyperoxia on expression mouse muscle and C2C12 myotubes. We found that hyperoxia‐mediated was associated with 5‐fold 3‐fold increase mRNA levels, respectively, Hyperoxia also enhanced...
Abstract Background Congenital Pulmonary Airway Malformation (CPAM) has an estimated prevalence between 0.87 and 1.02/10,000 live births little is know about their pathogenesis. To improve our knowledge on these rare malformations, we analyzed the cellular origin of two most frequent CPAM, CPAM types 1 2, compared malformations with adjacent healthy lung human fetal lungs. Methods We prospectively enrolled 21 infants undergoing surgical resection for CPAM. Human samples were collected after...
Mouse lung developmental maturation and final alveolarization phase begin at birth. During this dynamic process, alveolar cells modify their morphology anchorage to the extracellular matrix. In particular, epithelial cell (AEC) type I undergo cytoplasmic flattening folding ensure alveoli lining. We developed FACS conditions for simultaneous isolation of endothelial in absence specific reporters during early middle phase. evidenced first time a pool extractable populations expressing high...
Acute fibrinous and organizing pneumonia (AFOP) is a recently described histologic pattern of diffuse pulmonary disease. In children, all cases reported to date have been fatal. this study, we describe the first nonfatal AFOP in child review literature.A 10-year-old boy developed very severe aplastic anemia (VSAA) after being admitted our hospital with fulminant hepatic failure unknown origin. A chest computed tomography scan revealed multiple lung nodules biopsy lesion showed signs AFOP....
We previously reported that exposure of mice to hyperoxia is characterized by extensive lung cell necrosis and apoptosis, mild inflammatory response, elevated circulating levels corticosterone. Administration hydroxycortisone acetate during aggravated injury. Using adrenalectomized (ADX) sham-operated (sham) mice, we studied the role glucocorticoids in hyperoxia-induced Lung damage was attenuated ADX as measured weight protein content bronchoalveolar lavage seen light microscopy. Mortality...