A5049817881- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Multiple Myeloma Research and Treatments
- Hemoglobinopathies and Related Disorders
- Chronic Lymphocytic Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- CNS Lymphoma Diagnosis and Treatment
- Blood groups and transfusion
- Renal cell carcinoma treatment
- Ovarian cancer diagnosis and treatment
- Cancer-related gene regulation
- Chronic Myeloid Leukemia Treatments
- Protein Degradation and Inhibitors
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Cancer-related molecular mechanisms research
- Iron Metabolism and Disorders
- Viral-associated cancers and disorders
- Platelet Disorders and Treatments
- Genetic Syndromes and Imprinting
- Hematological disorders and diagnostics
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Cancer, Hypoxia, and Metabolism
University of Ioannina
2016-2025
University Hospital of Ioannina
2015-2024
Thomas Jefferson University
2013-2024
University of Pisa
2023
Tel Aviv Sourasky Medical Center
2023
Tel Aviv University
2023
University of Perugia
2023
Soroka Medical Center
2023
Hudson Institute
2017
John Wiley & Sons (United States)
2017
Significance MicroRNAs (miRNAs) are small ∼22-nt RNAs that important regulators of posttranscriptional gene expression. Since their initial discovery, they have been shown to be involved in many cellular processes, and misexpression is associated with disease etiology. Currently, nearly 2,800 human miRNAs annotated public repositories. A key question miRNA research how harbored by the genome. To answer this question, we examined 1,323 short RNA sequence samples identified 3,707 novel miRNAs,...
Maternally expressed gene 3 (MEG3) is a maternally imprinted representing large noncoding RNA in which microRNAs (miRNAs) and small nucleolar RNAs are also hosted. It capable of interacting with cyclic AMP, p53, murine double minute 2 (MDM2) growth differentiation factor 15 (GDF15) playing role cell proliferation control. MEG3 expression under epigenetic control, aberrant CpG methylation has been observed several types cancer. Moreover, copy number loss reported as additional mechanism...
Arginine deprivation, either by nutritional starvation or exposure to ADI-PEG20, induces adaptive transcriptional upregulation of ASS1 and ASL in glioblastoma multiforme ex vivo cultures cell lines. This is blocked neoplasia-specific CpG island methylation gene, causing arginine auxotrophy death. In cells with methylated islands, ADI-PEG20 initially a protective autophagic response, but abrogation this chloroquine accelerates potentiates cytotoxicity. Concomitant the islands both ASL,...
Tumours lacking argininosuccinate synthetase-1 (ASS1) are auxotrophic for arginine and sensitive to amino-acid deprivation. Here, we investigated the role of ASS1 as a biomarker response arginine-lowering agent, pegylated deiminase (ADI-PEG20), in lymphoid malignancies. Although protein was largely undetectable normal malignant tissues, frequent hypermethylation promoter observed specifically latter. A good correlation between methylation, low mRNA, absence expression sensitivity ADI-PEG20...
A number of growth factors are involved in clonal haematopoietic expansion and their clinical significance patients with chronic myeloproliferative diseases requires further evaluation. Using enzyme-linked immunosorbent assays, we analysed serum levels interleukin (IL)-1a, IL-1b, IL-2, IL-6, the soluble IL-2 receptor alpha (sIL-2Ra), thrombopoietin (TPO), 25 individuals myelofibrosis myeloid metaplasia (MMM), 40 essential thrombocythaemia (ET), eight polycythaemia vera (PV), 10 leukaemia...
Novel prognostic biomarkers and therapeutic strategies are urgently required for malignant melanoma. Ecto-5-prime-nucleotidase (NT5E; CD73) overexpression has been reported in several human cancers. The mechanism(s) underlying deregulated expression the clinical consequences of changes not known. We used RT–PCR, qPCR, methylation-specific PCR pyrosequencing to analyse regulation NT5E melanoma cell lines primary metastatic melanomas. is subject epigenetic mRNA downregulated by...
For the anucleate platelet it has been unclear how well transcriptomes correlate among different donors or across RNA profiling platforms, and what transcriptomes' relationship is with proteome. We profiled transcriptome of 10 healthy young males (5 white 5 black) no notable clinical history using sequencing by Affymetrix microarray. found that abundance mRNA transcripts was highly correlated individuals, independently race employed technology. Our RNA-seq data showed these high...
Cancer is nowadays considered to be both a genetic and an epigenetic disease. The most well studied modification in humans DNA methylation; however it becomes increasingly acknowledged that methylation does not work alone, but rather linked other modifications, such as histone modifications. Epigenetic abnormalities are reversible result novel therapies by reversing effects being explored. biggest clinical impact of modifying agents neoplastic disorders thus far has been haematological...
Bcl-2 family proteins are important regulators of apoptosis and its antiapoptotic members, which overexpressed in many types cancer, high prognostic significance, establishing them as attractive therapeutic targets. Quercetin, a natural flavonoid, has drawn much attention because it exerts anticancer effects, while sparing normal cells. A multidisciplinary approach been employed herein, an effort to reveal mode action including dose–response antiproliferative activity induced effect,...
Abstract Transplant‐ineligible relapsed/refractory (rr) diffuse large B‐cell lymphoma (DLBCL) patients represent an unmet medical need. Polatuzumab vedotin (Pola), anti‐CD79b antibody‐drug‐conjugate (ADG), with bendamustine‐ rituximab(BR) has recently gained approval for these patients, both in the USA and Europe, based on GO29365 phase IIb trial. Real‐life data Pola are extremely limited. We report outcomes of 61 Greek who received Pola‐(B)R mainly within a compassionate use program....
Over the last few years, increased evidence has supported role of iron dysregulation in pathogenesis multiple sclerosis (MS), as is essential for myelin formation and oxidative phosphorylation. We studied indices metabolism, such serum iron, ferritin, transferrin soluble receptor (sTFR) levels 27 MS patients. Seven patients had chronic progressive active disease (CP-A), six stable (CP-S), ten relapsing—remitting (RR-A) four (RR-S) disease. sTFR were found to be significantly higher CP-A...
Resistance to platinum- and taxane-based chemotherapy remains a major clinical impediment effective management of epithelial ovarian cancer (EOC). To gain insights into resistance mechanisms, we compared gene confirmed expression patterns novel EOC cell lines selected for paclitaxel carboplatin resistance. Here, report that can be conferred by downregulation the Polo-like kinase Plk2. Mechanistic investigations revealed occurred at level transcription via associated DNA methylation CpG...