A5020862368- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- COVID-19 Impact on Reproduction
- Gestational Diabetes Research and Management
- Birth, Development, and Health
- Pancreatic function and diabetes
- Pregnancy and Medication Impact
- Immune Cell Function and Interaction
- Diabetes Treatment and Management
- Fatty Acid Research and Health
- Diet and metabolism studies
- Neonatal Respiratory Health Research
- Metabolomics and Mass Spectrometry Studies
- Genomics and Rare Diseases
- Genetic Associations and Epidemiology
- Liver Disease Diagnosis and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Immune responses and vaccinations
- Eosinophilic Esophagitis
- RNA modifications and cancer
- Diabetes Management and Research
- Adipose Tissue and Metabolism
- IL-33, ST2, and ILC Pathways
- Cancer, Lipids, and Metabolism
- Congenital Anomalies and Fetal Surgery
Swansea University
2017-2024
Universities UK
2020-2021
Abstract Fructose intake has increased substantially throughout the developed world and is associated with obesity, type 2 diabetes non-alcoholic fatty liver disease. Currently, our understanding of metabolic mechanistic implications for immune cells, such as monocytes macrophages, exposed to elevated levels dietary fructose limited. Here, we show that reprograms cellular pathways favour glutaminolysis oxidative metabolism, which are required support inflammatory cytokine production in both...
Augmented T cell function leading to host damage in autoimmunity is supported by metabolic dysregulation, making targeting immunometabolism an attractive therapeutic avenue. Canagliflozin, a type 2 diabetes drug, sodium glucose co-transporter (SGLT2) inhibitor with known off-target effects on glutamate dehydrogenase and complex I. However, the of SGLT2 inhibitors human have not been extensively explored. Here, we show that canagliflozin-treated cells are compromised their ability activate,...
Healthy pregnancy is accompanied by various immunological and metabolic adaptations. Maternal obesity has been implicated in adverse outcomes such as miscarriage, preeclampsia, gestational diabetes mellitus (GDM), while posing a risk to the neonate. There lack of knowledge surrounding maternal immune system. The objective this study was consider if changes are influenced obesity. Peripheral blood collected from fasted GDM-negative pregnant women at 26-28 weeks gestation. Analysis done using...
Abstract Mandatory maternal metabolic and immunological changes are essential to pregnancy success. Parallel in metabolism immune function make immunometabolism an attractive mechanism enable dynamic adaptation during pregnancy. Immunometabolism is a burgeoning field with the underlying principle being that cellular underpins cell function. With whole body of carbohydrates, protein lipids well recognised occur our growing understanding as determinant immunoinflammatory effector responses, it...
The lipid environment changes throughout pregnancy both physiologically with emergent insulin resistance and pathologically e.g., gestational diabetes mellitus (GDM). Novel mass spectrometry (MS) techniques applied to minimally processed blood might lend themselves monitoring changing profiles inform care decisions across pregnancy. In this study we use an intact-sandwich, MALDI-ToF MS method identify phosphatidylcholine (PC) lysophosphatidylcholine (LPC) species calculate their ratio as...
The pyruvate transporter MPC1 (mitochondrial carrier 1) acts as a tumour-suppressor, loss of which correlates with pro-tumorigenic phenotype and poor survival in several tumour types. In high-grade serous ovarian cancers (HGSOC), patients display copy number around 78% cases reduced mRNA expression. To explore the metabolic effect expression, we demonstrate that depleting HGSOC cell lines drives expression key proline biosynthetic genes; PYCR1, PYCR2 PYCR3, biosynthesis proline. We show...
Metabolic heterogeneity is a determinant of immune cell function.The normal physiological metabolic reprogramming pregnancy that ensures the fuel requirements mother and baby are met, might also underpin changes in immunity occur with manifest as altered responses to pathogens autoimmune disease symptoms.Using peripheral blood from pregnant women at term, we reveal monocytes lose M2like gain M1-like properties accompanied by reductions mitochondrial mass, maximal respiration cardiolipin...
Pregnancy is characterised by metabolic changes that occur to support the growth and development of fetus over course gestation. These can be classified into two distinct phases: an initial anabolic phase prepare adequate store substrates energy which are then broken down used during a catabolic meet energetic demands mother, placenta fetus. Dynamic readjustment immune homeostasis also feature pregnancy likely linked in substrate utilisation at this time. As cellular metabolism increasingly...
Background: Metabolic heterogeneity is a determinant of immune cell function. The normal physiological metabolic reprogramming pregnancy that ensures the fuel requirements mother and baby are met might also underpin changes in innate adaptive immunity occur with manifest as altered responses to pathogens autoimmune disease symptoms. Here, we use peripheral blood monocytes, susceptible microenvironmentally-determined metabolism, determine if provokes an profile underpins functional...
Potential protective effects of breast milk and amniotic fluid against novel coronavirus SARS-CoV-2.Authors: April Rees1, Stephen Turner2, Catherine Thornton1*1 Institute Life Science, Swansea University Medical School, Swansea, Wales, UK, SA2 8PP2 Aberdeen, The Applied Health Sciences, Scotland, UK AB24 3FXDisclosure: authors report no conflict interest.Funding: This work was supported by the EPSRC Impact Acceleration Account at Welsh Government Sêr Cymru III Tackling COVID-19...
Background: While risk of infection with SARS-CoV-2 is low to pregnant women and the fetus, there increased preterm birth admission into ICU. The fetus relatively protected against infection, cases vertical transmission being rare. Various receptors accessory molecules which are known regulate viral entry host cells have soluble versions could act as decoy traps. Following on from our previous findings regarding abundance some these in breast milk amniotic fluid, we show maternal-fetal...
Recently, a young Professor started lecture on genome wide association studies (GWAS) by saying that ‘you can disregard any data published before 2000 as obsolete’. In doing so, he consigned all observations made in the using candidate gene approach from 1980 to dustbin. One of course see his point view; why consider old if new technologies emerge producing exciting data? Remonstration with him one us (D.J.G.) coffee break was no avail; stuck firmly views obsolete would be much use an out...
Abstract Pregnant women undergo dynamic metabolic and immunologic changes to ensure provision of nutrients to, prevent rejection of, the fetus. The mother increases glucose production, intolerance insulin resistance support fetal-placental development transitions from lipid storage lipolysis meet her own energy demands. Maternal immunoregulation prevents fetal semi-allograft whilst maintaining protection against pathogens. Hypothesis Immunometabolic adaptation underpins maternal immune...
Augmented T-cell function leading to host damage in autoimmunity is supported by metabolic dysregulation. Targeting immunometabolism for the treatment of repurposing clinically approved modulators, such as those used treat people with type 2 diabetes (T2D), therefore an attractive avenue. Canagliflozin, a class newest T2D drug - sodium glucose co-transporter (SGLT2) inhibitors has known off-target effects including mitochondrial glutamate dehydrogenase (GDH) and complex I inhibition. To...
Healthy pregnancy is accompanied by various immunological and metabolic adaptations. Maternal obesity has been implicated in adverse outcomes such as miscarriage, preeclampsia, gestational diabetes mellitus (GDM), while posing a risk to the neonate. There lack of knowledge surrounding maternal immune system. The objective this study was consider if changes are sabotaged obesity. Peripheral blood collected from fasted GDM-negative pregnant women at 26-28 weeks gestation. Analysis done using...
Abstract Maternal obesity has been implicated in adverse pregnancy outcomes such as miscarriage, preeclampsia and gestational diabetes mellitus (GDM) poses a risk to the neonate. There is knowledge gap surrounding changes maternal immune system. Hypothesis: Obesity exacerbates systemic inflammation pregnant women at 28 weeks of gestation. Samples: Peripheral blood was collected from fasted 26–28 gestation; all were GDM-negative Results: Insulin (p=<0.0001) PAI-1 (p=0.0487) levels...