A5030156112- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Cholesterol and Lipid Metabolism
- Neuroscience and Neuropharmacology Research
- Peroxisome Proliferator-Activated Receptors
- Microbial Community Ecology and Physiology
- Biochemical Analysis and Sensing Techniques
- Pregnancy and preeclampsia studies
- Advanced Proteomics Techniques and Applications
- Reproductive System and Pregnancy
- Olfactory and Sensory Function Studies
- Protein Structure and Dynamics
- Receptor Mechanisms and Signaling
- Photosynthetic Processes and Mechanisms
- Drug Transport and Resistance Mechanisms
- Fatty Acid Research and Health
- Cannabis and Cannabinoid Research
- RNA and protein synthesis mechanisms
- Autophagy in Disease and Therapy
- Amino Acid Enzymes and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Regulation of Appetite and Obesity
Swansea University
2018-2025
Institute of Life Sciences
2023-2024
University of California, Irvine
2017
University of Pittsburgh
2017
Italian Institute of Technology
2017
University of Bari Aldo Moro
2010-2016
Siena Biotech (Italy)
2014
Abstract Cross-presentation is a critical function of dendritic cells (DCs) required for induction antitumor immune responses and success cancer immunotherapy. It established that tumor-associated DCs are defective in their ability to cross-present antigens. However, the mechanisms driving these defects still unknown. We find impaired cross-presentation largely associated with defect trafficking peptide–MHC class I (pMHC) complexes cell surface. tumor-bearing hosts accumulate lipid bodies...
Dysregulated cholesterol metabolism is implicated in a number of neurological disorders. Many sterols, including and its precursors metabolites, are biologically active important for proper brain function. However, spatial the resulting sterol distributions poorly defined. To better understand situ across complex functional regions brain, we have developed on-tissue enzyme-assisted derivatization combination with microliquid extraction surface analysis liquid chromatography-mass spectrometry...
Despite being a critical molecule in the brain, mass spectrometry imaging (MSI) of cholesterol has been under-reported compared to other lipids due difficulty ionizing sterol molecule. In present work, we have employed an on-tissue enzyme-assisted derivatization strategy improve detection brain tissue sections. We report distribution and levels across specific structures mouse model Niemann-Pick type C1 disease, during development. MSI revealed that adult mouse, is highest pons medulla how...
Objective: Cachexia is a complex metabolic syndrome frequently occurring in cancer patients and exacerbated by chemotherapy. In skeletal muscle of hosts, reduced oxidative capacity low intracellular ATP resulting from abnormal mitochondrial function were described. Methods: The present study aimed at evaluating the ability mitochondria-targeted compound SS-31 to counteract wasting altered metabolism C26-bearing (C26) mice either receiving chemotherapy (OXFU: oxaliplatin plus 5-fluorouracil)...
A simple and fast method of lipid analysis isolated intact mitochondria by means MALDI-TOF mass spectrometry is described. Mitochondria from bovine heart yeast have been employed to set up validate the new analysis. The mitochondrial suspension directly applied over target and, after drying, covered a thin layer 9-aminoacridine matrix solution. profiles acquired with this procedure contain all peaks previously obtained analyzing extracts TLC and/or spectrometry. novel allows quick, simple,...
Disability worsening in multiple sclerosis (MS) is linked to neurodegeneration. Cholesterol homeostasis essential for normal brain function. CYP46A1, crucial cholesterol turnover and reduced some neurodegenerative diseases, a potential neuroprotective target. We hypothesized that CYP46A1 downregulated MS brains dysbalance. Mass spectrometric analysis of sterols was performed from matched plasma cerebrospinal fluid (CSF) an all-female cohort (n = 32, mean age 33). status recorded at baseline...
A method of direct lipid analysis by MALDI mass spectrometry in intact membranes, without prior extraction/separation steps, is described. The purple membrane isolated from the extremely halophilic archaeon Halobacterium salinarum was selected as model membrane. Lyophilized were grinded with 9-aminoacridine (9-AA) dry matrix, and powder mixture crushed a mechanical die press to form thin pellet. Small pieces pellet then attached target directly analyzed. In parallel, individual...
The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means combined thin-layer chromatography and MALDI-TOF/MS analyses total lipid extract. 80–90% major polar lipids were represented archaeol (diethers) remaining part caldarchaeol (tetraethers). direct analysis on plate showed presence diphytanylglycerol analogues phosphatidylinositol phosphatidylglycerol, N -acetylglucosamine-diphytanylglycerol phosphate plus some different from those previously...
Barth syndrome (BTHS), an X-linked disease associated with cardioskeletal myopathy, neutropenia, and organic aciduria, is characterized by abnormalities of card-iolipin (CL) species in mitochondria. Diagnosis the often compromised lack rapid widely available diagnostic laboratory tests. The present study describes a new method for BTHS screening based on MALDI-TOF/MS analysis leukocyte lipids. This generates "CL fingerprint" allows quick simple assay relative levels CL monolysocardiolipin...
Abstract The use of the matrix 9‐aminoacridine has been recently introduced in matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectrometry analysis both anionic and cationic phospholipids. In present study, we take advantage this technique to analyze lipids porcine olfactory mucosa a membrane fraction enriched cilia. Thin‐layer chromatography (TLC) 31 P‐NMR analyses lipid extracts were also performed parallel. MALDI‐TOF‐MS allowed identification classes total...
Background and Purpose Effective treatments of nausea are limited. In this study we evaluated the ability peripherally restricted fatty acid amide hydrolase (FAAH) inhibitor, URB937, to suppress acute anticipatory in rats examined pharmacological mechanism effect. Experimental Approach We investigated potential URB937 (administered i.p.) reduce establishment lithium chloride‐induced conditioned gaping (model nausea) expression contextually‐elicited rats. The role CB 1 receptors, 2 receptors...
The lipid composition of Halobacillus halophilus was investigated by combined thin-layer chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses the total extract. Main polar lipids were found to be sulfoquinovosyldiacylglycerol phosphatidylglycerol, while cardiolipin a minor together with phosphatidic acid, alanyl-phosphatidylglycerol two not yet fully identified components. In addition residual lipids, associated denatured proteins after...
Introduction: Fatty acid ethanolamides (FAEs) are a family of lipid mediators that participate in host biological functions. Procedures for the quantitative analysis FAEs include organic solvent extraction from matrices (e.g., blood), followed by purification and subsequent quantitation liquid chromatography–mass spectrometry (LC/MS) or gas spectrometry. During validation process new method LC/MS samples, we observed unusually high levels FAE, palmitoylethanolamide (PEA), blank samples did...
Metabolic heterogeneity is a determinant of immune cell function.The normal physiological metabolic reprogramming pregnancy that ensures the fuel requirements mother and baby are met, might also underpin changes in immunity occur with manifest as altered responses to pathogens autoimmune disease symptoms.Using peripheral blood from pregnant women at term, we reveal monocytes lose M2like gain M1-like properties accompanied by reductions mitochondrial mass, maximal respiration cardiolipin...
We report changes of the content anionic phospholipids in Bacillus subtilis response to hypoxic conditions and inhibition terminal respiration. Cardiolipin accumulates rapidly when bacteria are suspended non‐growth medium under reduced aeration or exposed inhibitor cyanide; increase cardiolipin occurs at expense its precursor phosphatidylglycerol is temperature‐dependent. Depending on extent stress, membranes containing different levels can be isolated from B. cells. The NADH oxidase...
The lipid environment changes throughout pregnancy both physiologically with emergent insulin resistance and pathologically e.g., gestational diabetes mellitus (GDM). Novel mass spectrometry (MS) techniques applied to minimally processed blood might lend themselves monitoring changing profiles inform care decisions across pregnancy. In this study we use an intact-sandwich, MALDI-ToF MS method identify phosphatidylcholine (PC) lysophosphatidylcholine (LPC) species calculate their ratio as...