To characterize and explore the potential in extracellular biosynthesis of silver nanoparticles (AgNPs) by Penicillium chrysogenum Aspergillus oryzae to investigate antifungal effect chemically vs biologically synthesized AgNPs comparing with conventional drugs against Trichophyton rubrum.Chemically (Chem-AgNPs) coated polyvinylpyrrolidone (PVP) were chemical reduction method glucose PVP aqueous solution. Biologically (Bio-AgNPs) produced from cell-free filtrate P. MUM 03.22 A. 97.19. Among...

Fabric structures are prone to contamination with microorganisms, as their morphology and ability retain moisture creates a proper environment for growth. In this work, novel, easily processed cheap coating nylon fabric antimicrobial characteristics was developed. After plasma treatment, made render the surface more reactive sites, impregnated chitosan silver nanoparticles by simply dipping it into mixture of different concentrations both components. Silver were previously synthesized using...

Abstract Bloodstream infections (BSIs) are considered a major cause of death worldwide. Staphylococcus spp. one the most BSIs prevalent bacteria, classified as high priority due to increasing multidrug resistant strains. Thus, fast, specific and sensitive method for detection these pathogens is extreme importance. In this study, we have designed novel assay in blood culture samples, which combines advantages phage endolysin cell wall binding domain (CBD) probe with accuracy high-throughput...

Essential oils (EOs) extracted from Lavandula luisieri and Cymbopogon citratus were tested for their antifungal activity against ten clinical isolates of dermatophytes isolated cases tinea pedis. Inhibition conidial germination drug/EO combination assay on two ATCC reference strains Trichophyton rubrum mentagrophytes. EOs characterised by high amount oxygenated monoterpenes in composition. Strong was observed the majority strains, fungicidal demonstrated. Positive interaction between L. EO...

The purpose of this work is the development Ag/a:C and Ag–Au/a:C coatings for ureteral stents, to provide them with antimicrobial characteristics. Silver was selected because its well-known antibacterial properties, while gold included assess capacity accelerate silver ion release forming a galvanic couple between Au Ag. Thus, metallic (Ag) bimetallic clusters (Ag–Au) were produced by three different configurations: (i) unbalanced magnetron sputtering (conventional sputtering), (ii) plasma...

For periods up to 21 days human bone marrow was cultured in control conditions that favor the proliferation and differentiation of osteoblastic cells. The effect AISI 316L corrosion products corresponding major separate metal ions (Fe, Cr, Ni) were studied three different phases culture period order investigate effects cell populations representative cells stages differentiation. Toxicity consequences presence cultures evaluated by biochemical parameters (enzymatic reduction MTT, alkaline...

Foodborne outbreaks due to the consumption of ready-to-eat vegetables have increased worldwide, with Escherichia coli (E. coli) being one main sources responsible. Viable but nonculturable bacteria (VBNC) retain virulence even after some disinfection procedures and constitute a huge problem public health their non-detectability through conventional microbiological techniques. Flow cytometry (FCM) is promising tool in food microbiology as it enables distinction different physiological states...

This paper describes a critical evaluation of miniaturised colorimetric assay, using MTT (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) reduction, applied to protozoan viability testing. The toxic substances used were copper, zinc, Triton X-100 (a membrane surfactant) and cycloheximide (an inhibitor the protein synthesis). assay ciliate Tetrahymena pyriformis was optimised in terms concentration incubation time. Since protozoa are non adherent cells modified order maintain...

ABSTRACT Bacteriophages (phages) are ubiquitous entities present in every conceivable habitat as a result of their bacterial parasitism. Their prevalence and impact the ecology communities ability to control pathogens make characterization essential, particularly new phages, improving knowledge potential application. The isolation lytic phage against Sphaerotilus natans strain DSM 6575, named vB_SnaP-R1 (SnaR1), is here described. Besides being first sequenced genome infecting phage, 99% its...

Excessive eutrophication is a major water quality issue in lakes and reservoirs worldwide. This complex biological process can lead to serious problems. Although it be adequately addressed by applying sophisticated mathematical models, the application of these tools reservoir management context requires significant amounts data large computation times. work presents simple primary production model calibration procedure that efficiently used operational frameworks. It considers four state...

Collaborations between different Microbiological Resource Centres (mBRCs) and ethical sourcing practices are mandatory to guarantee biodiversity conservation, successful sustainable preservation fair share of benefits that arise from the use genetic resources. Since microbial Culture Collections (CCs) now engaged in meeting high quality operational standards, they facing challenge establishing control criteria certify their biological materials. The authentication/certification strains is...

—The blue and red shrimp, Aristeus antennatus (Risso, 1816), the stout virilis (Bate, 1881), represent two of most valuable demersal deep waters shrimp species subject to exploitation in Mozambique. This paper analyses distribution, abundance biological parameters these for first time this area. During 13th March 10th April a survey on board R/V Vizconde de Eza has been carried out assess water crustacean resources Mozambique waters. The sampling design followed random stratified scheme with...

To improve bead ingestion counts in Tetrahymena pyriformis by automated image analysis as an alternative to direct-counts.Fluorescent latex beads were added T. cultures for tests. The number of ingested 25 cells was counted directly epifluorescence microscopy and compared with similar data from analysis. anova indicated that not significantly different (P < 0.05). particularly provided advantages terms speed.The is superior direct counting speed analysis.The method very rapid will allow many...

Objetivo: identificar as práticas integrativas e complementares disponibilizadas nos serviços do SUS no estado Rio Grande Sul. Método: abordagem quantitativa, descritiva-exploratória com uso de dados secundários disponibilizados Cadastro Nacional Estabelecimentos Saúde, os foram extraídos mês novembro 2022. Resultados: dentre 497 municípios Sul, apenas 161 dispõe SUS, onde 69 ofertam acupuntura, 11 antroposofia aplicada a saúde, 20 aromaterapia, ayurveda, 36 fitoterapia, 15 homeopatia, 29...