A5029059099- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Identification and Quantification in Food
- Enterobacteriaceae and Cronobacter Research
- Spectroscopy and Chemometric Analyses
- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
- Microbial Inactivation Methods
- Food Safety and Hygiene
- Essential Oils and Antimicrobial Activity
- Microbial Metabolic Engineering and Bioproduction
- Probiotics and Fermented Foods
- Fungal and yeast genetics research
- Escherichia coli research studies
- Parasitic Infections and Diagnostics
- Bacteriophages and microbial interactions
- Fermentation and Sensory Analysis
- Plant Virus Research Studies
- Enzyme Structure and Function
- Microbial Metabolism and Applications
- Antimicrobial agents and applications
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Medical Device Sterilization and Disinfection
- Gut microbiota and health
Food Research Institute
2011-2024
National Agricultural and Food Centre
2015-2024
Journal Article Limitation in the detection of Listeria monocytogenes food presence competing innocua Get access K. Oravcová, Oravcová Department Microbiology and Molecular Biology Food Research Institute, Bratislava, Slovakia Search for other works by this author on: Oxford Academic Google Scholar T. Trnčíková, Trnčíková Kuchta, Kuchta E. Kaclíková Kaclíková, Biology, PO Box 25, SK‐82475 Slovakia. E‐mail: kaclikova@vup.sk Applied Microbiology, Volume 104, Issue 2, 1 February 2008, Pages...
A new real-time polymerase chain reaction-based method was developed for the detection of Salmonella enterica in food.The consisted a novel two-step enrichment involving overnight incubation buffered peptone water and 5-h subculture Rappaport-Vassiliadis medium, lysis bacterial cells Salmonella-specific 5'-nuclease PCR with an exogenous internal amplification control. Because used, limit dead S. artificially contaminated ice cream salami samples high at 10(7 )CFU (25 g)(-1), eliminating...
Abstract This work was aimed at determining the occurrence and diversity of Listeria monocytogenes in a traditional meat‐processing facility to reveal persistent contamination. A total 268 samples, including 196 environmental samples 72 meat were collected during four‐year period, 70 found be L. positive. Molecular serotyping 77 isolates classified these strains into four serogroups, with majority 34 serogroup IIa. To contamination, subtyping by AscI/ApaI‐PFGE applied. Cluster analysis...
A new real-time PCR-based method was developed for the detection of Listeria monocytogenes in food.A two-step enrichment involving a 24-h incubation half-Fraser broth followed by 6-h subculture Fraser used, cell lysis and PCR with primers TaqMan probe previously our laboratory. When evaluated 144 naturally contaminated food samples, 44 were detected as positive 42 standard EN ISO 11290-1. With 61 samples artificially at level 10(0) CFU per 25 g, 58 respective methods.The facilitated L. on...
Journal Article A single‐tube nested real‐time polymerase chain reaction for sensitive contained detection of Cryptosporidium parvum Get access J. Minarovičová, Minarovičová Department Microbiology and Molecular Biology, Food Research Institute, Bratislava, Slovakia Search other works by this author on: Oxford Academic Google Scholar E. Kaclíková, Kaclíková K. Krascsenicsová, Krascsenicsová P. Siekel, Siekel T. Kuchta Tomáš Kuchta, Priemyselná 4, PO Box 25, SK‐82475 Bratislava 26, Slovakia....
Cronobacter spp. (formerly Enterobacter sakazakii) is responsible for rare but fatal cases of infection in neonates and immunocompromised infants. The aim our study was to characterize strains isolated from powdered infant foods Public Health Authority the Slovak Republic 2009-2010. Powdered food products have been analysed using currently available standard method ISO/TS 22964: 2006 detection complemented with qPCR confirmation positive strains. Thirteen were more than 900 formulae,...
The aim of this study was to develop a 5'-nuclease polymerase chain reaction (PCR) for the rapid detection and quantification Listeria monocytogenes.Specific primers fluorogenic probe were designed, which target specific sequence actA gene encoding protein involved in actin filament assembly. PCR system highly sensitive L. monocytogenes (inclusivity, 100%; exclusivity, 100%), determined using 46 28 non-L. strains. Detection limits 10(4) cfu ml(-1) after 35 cycles 10(2) 45 achieved by both...
A kinetic 5'-nuclease polymerase chain reaction (real-time PCR) for the quantification of Escherichia coli was developed.Specific primers and a fluorogenic probe oriented to sfmD gene, encoding putative outer membrane export usher protein, were designed. The PCR system highly specific sensitive E. coli, as determined with 37 non-E. strains (exclusivity, 100%) 24 (inclusivity, 100%). When used in real-time PCR, linear calibration lines obtained range from 10(2) 10(8) CFU ml(-1) three strains....
is an important pathogen responsible for listeriosis, a serious foodborne illness associated with high mortality rates. Therefore,
Ewe's milk farm production is permanently associated with the risk of contamination by pathogenic bacteria, including Listeria monocytogenes. In present study, prevalence and diversity L. monocytogenes strains repeatedly isolated from tank ewe's milking environment on a in Slovakia during prolonged period were investigated to identify source potentially persistent contamination. A total 140 samples along chain collected an 18-month period. From all these samples, 45 found positive 90.3%...
A nuclear mutant of Saccharomyces cerevisiae deficient in mitochondrial fumarase has been identified through the vitro biochemical assay enzyme activity after visual selection due to an increased acidification ability its colonies. Cells fumarase-deficient fermenting glucose accumulated extracellular fumaric acid. This accumulation was observed only growing cultures and required functional electron transport from succinate dehydrogenase oxygen.