Abstract Background Nanoparticles are small-scale substances (<100 nm) with unique properties. Therefore, nanoparticles pose complex health risk implications. The objective of this study was to detect whether treatment quercetin (Qur) and/or arginine (Arg) ameliorated nephrotoxicity induced by two different doses nano zinc oxide (n-ZnO) particles. Method ZnO were administered orally in (either 600 mg or 1 g/Kg body weight/day for 5 conscutive days) Wister albino rats. In order the...

Human bone marrow-derived stromal stem cells (hBMSC) exhibit multiple functions, including differentiation into skeletal (progenitor function), hematopoiesis support, and immune regulation (nonprogenitor function). We have previously demonstrated the presence of morphological functional heterogeneity hBMSC cultures. In present study, we characterized in detail two hTERT-BMSC clonal cell populations termed here CL1 CL2 that represent an opposing phenotype with respect to morphology, markers...

TGF β is a potent regulator of several biological functions in many cell types, but its role the differentiation human bone marrow-derived skeletal stem cells (hMSCs) currently poorly understood. In present study, we demonstrate that single dose 1 prior to induction osteogenic or adipogenic results increased mineralized matrix numbers lipid-filled mature adipocytes, respectively. To identify mechanisms underlying this -mediated enhancement lineage commitment, compared gene expression...

The aim of the present real time in vivo micro-computed tomography (µCT) and histologic experiment was to assess efficacy guided bone regeneration (GBR) around standardized calvarial critical size defects (CSD) using marrow-derived mesenchymal stem cells (BMSCs), collagen membrane (CM) with without tricalcium phosphate (TCP) graft material. In calvaria nine female Sprague-Dawley rats, full-thickness CSD (diameter 4.6 mm) were created under general anesthesia. Treatment-wise, rats divided...

Abstract Bone marrow stromal (Mesenchymal) stem cells (MSCs) are multipotent bone capable of differentiating into mesoderm-type cells, such as osteoblasts and adipocytes. Existing evidence suggests that transformation MSCs gives rise to sarcoma. In order identify the molecular mechanism leading spontaneous human (hBMSCs), we performed comprehensive microRNA (miRNA) mRNA profiling in transformed hBMSC-Tum line compared parental clone. As a result, identified multiple dysregulated networks...

Hedgehog (Hh) signaling is essential for osteoblast differentiation of mesenchymal progenitors during endochondral bone formation. However, the critical role Hh adult remodeling remains to be elucidated. A Smoothened (SMO) antagonist/Hedgehog inhibitor, BMS-833923, identified a functional screening stem cell small molecule library, was investigated its effects on human skeletal (mesenchymal) cells (hMSC). Alkaline phosphatase (ALP) activity and Alizarin red staining were employed as markers...

Background: The efficacy of an extract from date seeds has been tested successfully on the glycemic control type I diabetes mellitus in rats. A suggestion that seed could stimulate certain cells to differentiate into insulin-secreting proposed. In order investigate such a possibility, this study was conducted measure C-peptide levels serum 1 diabetic rats treated with extract.Methods: Two hundred were divided 4 groups. Group served as control. II given daily ingestions 10 ml extract. Groups...

Abstract TGF-β1, a multifunctional regulator of cell growth and differentiation, is the most abundant bone matrix factor. During differentiation human stromal cells (hBMSCs), which constitute marrow osteoblast (OS) adipocyte (AD) progenitor cells, continuous TGF-β1 (10 ng/ml) treatment enhanced OS as evidenced by increased mineralised production. Conversely, pulsed administration during commitment phase mature lipid-filled numbers. Global gene expression analysis using DNA microarrays in...

Chemical biology approaches using small molecule inhibitors targeting specific signaling pathways are useful tools to dissect the molecular mechanisms governing stem cell differentiation and for their possible use in therapeutic interventions. Stem library functional screen was performed employing human bone marrow skeletal (mesenchymal) cells (hBMSCs). Alkaline phosphatase (ALP) activity formation of mineralized matrix visualized by Alizarin red staining were employed as markers...

Background: Embryonic stem cells (ESCs) have the potential to form teratomas when implanted into immunodeficient mice, but data in immunocompetent mice are limited. We therefore investigated teratoma formation after implantation of three different mouse ESC (mESC) lines mice. Materials and Methods: BALB/c were injected with highly germline competent mESCs (129Sv, C57BL/6) subcutaneously or under kidney capsule. After 4 weeks, euthanized examined histologically for development. The incidence,...

Acetaminophen (APAP) hepatotoxicity is characterised by an extensive oxidative stress due to depletion of glutathione (GSH), which results in massive lipid peroxidation and subsequent liver injury. The current paradigm suggests that mitochondria are the main source reactive oxygen species (ROS), impair mitochondrial function responsible for cell signalling resulting death. This study was designed compare potential impact thymoquinone (THQ), and/or curcumin (CURC) on injury induced APAP...

Better understanding of the signaling pathways that regulate human bone marrow stromal stem cell (hBMSC) differentiation into bone-forming osteoblasts is crucial for their clinical use in regenerative medicine. Chemical biology approaches using small molecules targeting specific are increasingly employed to manipulate fate. We alkaline phosphatase activity and staining assays assess osteoblast Alizarin R mineralized matrix formation cultured hBMSCs. Changes gene expression were assessed an...

Guided bone regeneration (GBR) using a porcine-derived collagen matrix (Mucograft [MG], Geistlich) has not yet been reported. The aim of this histologic and biomechanical study was to compare the efficacy MG versus resorbable membranes (RCMs) in facilitating GBR around standardized rat calvarial defects. Forty female Wistar albino rats with mean age weight 6 9 weeks 250 300 g, respectively, were used. With under general anesthesia, skin over calvaria exposed full-thickness flap. A...

This study was performed to investigate the effect of curcumin on cardiac myosin-induced autoimmune myocarditis in rats and change thioredoxin (TRX) immunoreactivity cardiomyocytes following treatment. Twenty-four six-week-old male Wistar were randomly allocated into 4 groups 6 each. Group I received neither nor myosin. II an oral solution 1 g/kg/day daily, from day 21. To induce myocarditis, animals both group III IV injected by mg porcine myosin days 8. In addition, Serum levels creatine...

Several studies have demonstrated the multipotentiality of human neonatal foreskin stromal cells (hNSSCs) as being able to differentiate into adipocytes and osteoblasts potentially other cell types. Recently, we that hNSSCs play a role during in vitro angiogenesis appear possess capacity endothelial-like cells; however, their angiogenic potential within an ex vivo environment remains unclear. Current study shows display significant migration undifferentiated state high responsiveness wound...

Interstitial cells of Cajal (ICC) are widely distributed in human gastrointestinal (GI) tract, especially the layer muscularis externa between neurons and smooth muscles. They play a very important role coordination GI tract motility. The aims this research were to study morphology distribution ICC using immunohistochemistry staining methods, determine immune reactivity anoctamin 1 (Ano1) compared with c-Kit, if Ano1 is reliable marker for tract.Specimens from wall stomach, small intestine,...

The objective of the present work was to study changes in collagen type I and II distribution articular cartilage immobilised remobilised rabbit knee joints. Twenty-four adult male rabbits were divided into three groups. One groups a control group with free movement. right joints animals other two for 4 weeks, followed by period 10 weeks remobilisation 3. Collagen tibial medial condyle joint estimated immunohistochemically all A degenerative process evident after immobilisation leading...

The outcome of the autograft therapy for Parkinson's disease including autologous cells from adrenal medulla was disappointing. This could be attributed to pathological process in affecting medulla. study performed investigate histopathological changes AS/AGU rat, a model disease, comparison with Albino Swiss (AS) rats.A total 24 male AS rats were divided into four groups, each 6 animals: W1 - aged 1 week; adult rats; AS/ /AGU and rats. sacrificed glands dissected processed histological...

The study presented an extremely rare case of real complete bilateral duplication inferior vena cava (IVC) in a male cadaver which has never been reported before. Both IVC had approximately the same diameter. right drained into atrium; left continued as hemiazygos vein and superior cava. Three anastomotic venous channels, cranial preaortic, middle caudal retroaortic, joined both vessels. Multiple variations way drainage posterior intercostal veins, on sides, were also present. present report...