Regenerative medicine is a novel approach for treating conditions in which enhanced bone regeneration required. We identified transgelin (TAGLN), transforming growth factor beta (TGFβ)-inducible gene, as an upregulated gene during vitro osteoblastic and adipocytic differentiation of human marrow-derived stromal (skeletal) stem cells (hMSC). siRNA-mediated silencing TAGLN impaired lineage into osteoblasts adipocytes but cell proliferation. Additional functional studies revealed that...

The Arabian camel is the largest known mammal that can survive in severe hot climatic conditions. We provide molecular explanation for thermotolerance of granulosa somatic cells after exposure to 45 °C 2 (acute heat shock) or 20 h (chronic shock). common features cellular responses acute stress were increase shock proteins and DNA repair enzymes expression. Actin polymerization Rho signaling critically activated as a defense against shock. Cells exposed chronic showed altered cell...

Human bone marrow-derived stromal stem cells (hBMSC) exhibit multiple functions, including differentiation into skeletal (progenitor function), hematopoiesis support, and immune regulation (nonprogenitor function). We have previously demonstrated the presence of morphological functional heterogeneity hBMSC cultures. In present study, we characterized in detail two hTERT-BMSC clonal cell populations termed here CL1 CL2 that represent an opposing phenotype with respect to morphology, markers...

Abstract Colorectal cancer (CRC) is the fourth most common type globally. Investigating signaling pathways that maintain cell phenotype can identify new biomarkers for targeted therapy. Aberrant transforming growth factor-β (TGFβ) has been implicated in CRC progression, however, exact mechanism by which TGFβ exerts its function still being unraveled. Herein, we investigated TAGLN expression, prognostic value, and regulation CRC. While was generally found to be downregulated CRC, elevated...

Understanding the regulatory networks underlying lineage differentiation and fate determination of human bone marrow stromal cells (hBMSC) is a prerequisite for their therapeutic use. The goal current study was to unravel novel role low-density lipoprotein receptor-related protein 3 (LRP3) in regulating osteogenic adipogenic immortalized hBMSCs. Gene expression profiling revealed significantly higher LRP3 levels highly hBMSC clone imCL1 than less imCL2, as well significant upregulation...

TGF β is a potent regulator of several biological functions in many cell types, but its role the differentiation human bone marrow-derived skeletal stem cells (hMSCs) currently poorly understood. In present study, we demonstrate that single dose 1 prior to induction osteogenic or adipogenic results increased mineralized matrix numbers lipid-filled mature adipocytes, respectively. To identify mechanisms underlying this -mediated enhancement lineage commitment, compared gene expression...

Debridement and disinfection of the root canal system is a crucial step in endodontic procedures. The effectiveness irrigation relies on both mechanical flushing action ability irrigants to dissolve tissue kill bacteria. objective present study evaluate compare cytotoxicity QMix™ irrigating solution immortalized human bone marrow mesenchymal stem cells (hTERT-MSC-C1) it with that sodium hypochlorite (NaOCl). Immortalized (hTERT-MSCs) were exposed NaOCl. Cell viability was assessed by 3-(4,...

Abstract TGF-β1, a multifunctional regulator of cell growth and differentiation, is the most abundant bone matrix factor. During differentiation human stromal cells (hBMSCs), which constitute marrow osteoblast (OS) adipocyte (AD) progenitor cells, continuous TGF-β1 (10 ng/ml) treatment enhanced OS as evidenced by increased mineralised production. Conversely, pulsed administration during commitment phase mature lipid-filled numbers. Global gene expression analysis using DNA microarrays in...

Abstract Targeting regulatory signaling pathways that control human bone marrow stromal (skeletal or mesenchymal) stem cell (hBMSC) differentiation and lineage fate determination is gaining momentum in the regenerative medicine field. Therefore, to identify central mechanism of osteoblast hBMSCs, molecular phenotypes two clonal hBMSC lines exhibiting opposite vivo phenotypes, namely, forming (hBMSC +bone ) non-bone −Bone cells, were studied. Global transcriptome analysis revealed significant...

All-trans retinoic acid (RA) is a metabolite of vitamin A and has pleiotropic actions on many different biological processes, including cell growth differentiation, involved in aspects fertility developmental biology. In the current study, we investigated effects RA camel (Camelus dromedarius) cumulus-oocyte complex vitro maturation (IVM). IVM medium was supplemented with 0, 10, 20, 40 µM RA. Application 20 significantly reduced proportion degenerated oocytes improved oocyte meiosis first...

Derivation of bone forming cells (osteoblasts) from human embryonic stem (hESCs) is a prerequisite for their use in clinical applications. However, there no standard protocol differentiating hESCs into osteoblastic cells. The aim this study was to identify the emergence stromal (mesenchymal and skeletal) cell (hMSC)-like population, known be precursors test differentiation capacity ex vivo cultures vivo. We cultured feeder-free environment using serum replacement as suspension aggregates...

Background: Embryonic stem cells (ESCs) have the potential to form teratomas when implanted into immunodeficient mice, but data in immunocompetent mice are limited. We therefore investigated teratoma formation after implantation of three different mouse ESC (mESC) lines mice. Materials and Methods: BALB/c were injected with highly germline competent mESCs (129Sv, C57BL/6) subcutaneously or under kidney capsule. After 4 weeks, euthanized examined histologically for development. The incidence,...

Smoking and the severity of periodontal disease have long been associated. In Saudi Arabia, tobacco smoking is rising, contributing to increased demand for products that counter its detrimental effects. The antioxidant properties vitamin C (vit C) make it a powerful countermeasure toxicity. Observation these effects on human gingival fibroblasts (hGFs) would suggest use in future dental applications.To examine proliferation, adhesion, expression extracellular RNA extracted from cigarette...

The fine structures of mouse embryonic stem cells (mESCs) grown as colonies and differentiated in three-dimensional (3D) culture embryoid bodies (EBs) were analyzed by transmission electron microscopy. Undifferentiated mESCs expressed markers that proved their pluripotency. Differentiated EBs different differentiation marker proteins from the three germ layers. ultrastructure revealed presence microvilli on cell surfaces, large deep infolded nuclei, low cytoplasm-to-nuclear ratios, frequent...

Various materials have been used to promote human dental pulp stem cells (hDPSCs) differentiation produce dentin bridge formation with less-than-optimal results. Polydatin (PD), a naturally present material osteogenic properties can be promising in the regeneration/repair process. The aim of this study was evaluate effect (PD) on viability and cells. PD hDPSCs terms cellular viability, alkaline phosphatase (ALP) production, messenger RNAs (mRNA) odontogenic markers production using...

We studied the potential of human bone marrow-derived mesenchymal stem cell conditioned media (hBMSC CM) in protecting endothelial properties (viability, proliferation, and migrations) from deleterious effects produced by inflammatory environment H

Background: Osteoporosis is associated with a metabolic imbalance between adipogenesis and osteogenesis. We hypothesized that implanting carrier for differentiated stem cells signaling molecules inside adipose tissues could be used to enable transdifferentiation cells, upregulate osteogenesis, support bone formation, which may regain the balance osteogenesis adipogenesis. Methodology: A CL1 human mesenchymal cell line was grown in an osteogenic medium differentiate into osteoblasts, were...