A5054916925- Animal Genetics and Reproduction
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Chromatin Remodeling and Cancer
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic Syndromes and Imprinting
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Retinal Development and Disorders
- Muscle Physiology and Disorders
- Planarian Biology and Electrostimulation
- Gender Studies in Language
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
Huazhong Agricultural University
2019-2024
Shandong Agricultural University
2023-2024
Chinese Academy of Agricultural Sciences
2021
Agricultural Genomics Institute at Shenzhen
2021
Expression of key transcription factors can induce transdifferentiation in somatic cells; however, this conversion is usually incomplete due to undefined intrinsic barriers. Here, we employ MyoD-induced fibroblasts as a model illustrate the chromatin structures that impede cell-fate transition. Focusing on three-dimensional (3D) interactions, show MyoD directly establishes loops activate myogenic transcriptional program. Similarly, dynamic changes CTCF-mediated interactions are favorable for...
Skeletal muscle myogenesis is a sophisticated process controlled by genetic and epigenetic regulators. In animals, one of the key enzymes for DNA demethylation 5-methylcytosine TET2. Although TET2 essential development, mechanisms which regulates myogenesis, particularly implication stem cells, remains unclear. present study, we employed knockout mouse model to investigate function in development regeneration. We observed that deficiency caused impaired cell proliferation differentiation,...
Salamanders are unique among tetrapods in their ability to regenerate limbs throughout life. Like other poikilothermic amphibians, salamanders also show a remarkable capacity survive long periods of starvation. Whether the physiological reserves necessary for tissue regeneration preserved or sacrificed starved is unknown. In current study, we maintained Iberian ribbed newts ( Pleurodeles waltl) under extreme stress assess extent and identify molecular cellular changes that may occur such...
Abstract Muscle stem cells (MuSCs) are essential for skeletal muscle development and regeneration, ensuring integrity normal function. The myogenic proliferation differentiation of MuSCs orchestrated by a cascade transcription factors. In this study, we elucidate the specific role factor 12 (Tcf12) in regeneration based on loss-of-function studies. Muscle-specific deletion Tcf12 cause weight loss owing to reduction myofiber size during development. Inducible specifically adult delayed...
Abstract All female vertebrates develop a pair of ovaries except for birds, in which only the left gonad develops into an ovary, whereas right regresses. Previous studies found that transcription factor Paired-Like Homeodomain 2 (PITX2), key mediator left/right morphogenesis vertebrates, was also implicated asymmetric gonadal development chickens. In this study, we systematically screened and validated signaling pathways could be targeted by Pitx2 to control unilateral development....
Abstract Background Birds exhibit a unique asymmetry in terms of gonad development. The female left generates functional ovary, whereas the right regresses. In males, both and gonads would develop into testes. How is this left/right established only females but not males remains unknown. epigenetic regulation gonadal developmental genes may contribute to sex disparity. modification histone tails such as H3K27ac tightly coupled chromatin activation gene expression. To explore whether marked...
Brown adipose tissue (BAT) is responsible for non-shivering thermogenesis, but it absent in some mammals, including pigs. During development, BAT progenitors are derived from paired box 7 (Pax7)-expressing somitic mesodermal stem cells, which also give rise to skeletal muscle. However, the intrinsic mechanisms underlying fate decisions between brown fat and muscle remain elusive. In this study, we analyzed dynamics of chromatin landscape during segregation specification lineages Pax7
Loss-of-function approaches provide strong evidence for determining the role of particular genes. The prevalent CRISPR/Cas9 technique is widely used to disrupt target gene with uncontrolled non-homologous end joining after double strand breaks, which results in mosaicism and multiple genotypes founders. In animal models long generation time such as salamanders, producing homozygous offspring mutants would be rather labor intensive consuming. Here we utilized base editing create...