Alexandre Dayer
A5075365580- Bipolar Disorder and Treatment
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Attention Deficit Hyperactivity Disorder
- Receptor Mechanisms and Signaling
- Child and Adolescent Psychosocial and Emotional Development
- Genetics and Neurodevelopmental Disorders
- Functional Brain Connectivity Studies
- Genetic Associations and Epidemiology
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Axon Guidance and Neuronal Signaling
- Genetic Syndromes and Imprinting
- Personality Disorders and Psychopathology
- Adolescent and Pediatric Healthcare
- Neural dynamics and brain function
- Stress Responses and Cortisol
- Epigenetics and DNA Methylation
- Tryptophan and brain disorders
- Neuroendocrine regulation and behavior
- Child Abuse and Trauma
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Mental Health Research Topics
- MicroRNA in disease regulation
- Autism Spectrum Disorder Research
University Hospital of Geneva
2015-2024
University of Geneva
2014-2023
Genomics (United Kingdom)
2022
University of Cambridge
2021-2022
Geneva College
2008-2019
Hôpital Beau-Séjour
2017
Manchester Metropolitan University
2015
Inserm
2014
Université de Strasbourg
2014
University of Liège
2014
Abstract New neurons continue to be generated in the dentate gyrus throughout adulthood. Previous studies have shown that a significant proportion of new granule cells labeled with thymidine analogue bromodeoxyuridine (BrdU) are lost from adult within 2 weeks. How long this loss continues and extent which it represents cell death, as opposed dilution label, is unclear. To address these questions, rats were injected BrdU, BrdU labeling was compared at several survival time points. Double...
Ongoing neurogenesis in the adult mammalian dentate gyrus and olfactory bulb is generally accepted, but its existence other brain regions highly controversial. We labeled newly born cells rats with S-phase marker bromodeoxyuridine (BrdU) used neuronal markers to characterize new at different time points after cell division. In neocortex striatum, we found BrdU-labeled that expressed each of eight markers. Their size as well staining for gamma-aminobutyric acid (GABA), glutamic decarboxylase...
During corticogenesis, distinct subtypes of neurons are sequentially born from ventricular zone progenitors. How these cells molecularly temporally patterned is poorly understood. We used single-cell RNA sequencing at high temporal resolution to trace the lineage molecular identities successive generations apical progenitors (APs) and their daughter in mouse embryos. identified a core set evolutionarily conserved, genes that drive APs internally driven more exteroceptive states. found...
Tracking neuronal transcriptional programs Early in brain development, cortical neurons are born near the ventricles, then migrate to their functional destinations. Telley et al. used a fluorescent labeling technique see what transcripts characterize these earliest stages of neural development. Waves initiated, passed by as neuron progresses from proliferative migratory and finally connectivity phases. Science , this issue p. 1443
Recent observations demonstrate that anesthetics rapidly impair synaptogenesis during neuronal circuitry development. Whether these effects are lasting and depend on the developmental stage at which drugs administered remains, however, to be explored.Wistar rats received propofol anesthesia defined stages early postnatal life. The acute long-term of treatments cytoarchitecture were evaluated by Neurolucida confocal microscopy analysis after iontophoretic injections Lucifer Yellow into layer...
Background Recent experimental observations suggest that, in addition to induce neuroapoptosis, anesthetics can also interfere with synaptogenesis during brain development. The aim of this study was pursue issue by evaluating the exposure time-dependent effects volatile on neuronal cytoarchitecture 16-day-old rats, a developmental stage characterized intense cerebral cortex. Methods Whistar rats underwent isoflurane (1.5%), sevoflurane (2.5%), or desflurane (7%) anesthesia for 30, 60, and...
Background An increasing number of clinical observations suggest adverse neurologic outcome after methylene blue (MB) infusion in the setting parathyroid surgery. Hence, aim current study was to investigate potentially neurotoxic effects MB using a combination vivo and vitro experimental approaches. Methods Isoflurane-anesthetized adult rats were used evaluate impact single bolus intravascular administration on systemic hemodynamic responses minimum alveolar concentration (MAC) isoflurane...
Experience-driven activity plays an essential role in the development of brain circuitry during critical periods early postnatal life, a process that depends upon dynamic balance between excitatory and inhibitory signals. Since general anesthetics are powerful pharmacological modulators neuronal activity, important question is whether how these drugs can affect synaptic networks. To address this issue, we examined here impact on synapse growth dynamics. We show exposure young rodents to...
<h3>Importance</h3> Lithium is a first-line mood stabilizer for the treatment of bipolar affective disorder (BPAD). However, efficacy lithium varies widely, with nonresponse rate up to 30%. Biological response markers are lacking. Genetic factors thought mediate lithium, and there previously reported genetic overlap between BPAD schizophrenia (SCZ). <h3>Objectives</h3> To test whether polygenic score SCZ associated in explore potential molecular underpinnings this association. <h3>Design,...
The neuron-specific K-Cl cotransporter, KCC2, is highly expressed in the vicinity of excitatory synapses pyramidal neurons, and recent vitro data suggest that this protein plays a role development dendritic spines. vivo relevance these observations is, however, unknown. Using utero electroporation combined with post hoc iontophoretic injection Lucifer Yellow, we show premature expression KCC2 induces significant permanent increase spine density layer 2/3 neurons somatosensory cortex....
Serotonin 3A receptor (5-HT3A R) is associated at the genetic and epigenetic levels with a variety of psychiatric disorders interacts early-life stress such as childhood maltreatment. We studied impact maltreatment on methylation status 5-HT3A R its association clinical severity outcomes in relation functional polymorphism.Clinical indexes 346 bipolar, borderline personality, adult attention deficit hyperactivity patients were tested for DNA eight gene CpGs. Relationship between variant...
Early life adversity plays a critical role in the emergence of borderline personality disorder ( BPD ) and this could occur through epigenetic programming. In perspective, we aimed to determine whether childhood maltreatment durably modify processes by means whole‐genome methylation scan subjects. Using Illumina Infinium® HumanMethylation450 BeadChip , global status DNA extracted from peripheral blood leucocytes was correlated severity 96 subjects suffering high level child 93 major...
Abnormal patterns of electrical oscillatory activity have been repeatedly described in adult ADHD. In particular, the alpha rhythm (8-12 Hz), known to be modulated during attention, has previously considered as candidate biomarker for present study, we asked ADHD patients self-regulate their own using neurofeedback (NFB), order examine modulation oscillations on attentional performance and brain plasticity. Twenty-five 22 healthy controls underwent a 64-channel EEG-recording at resting-state...
Lithium is the gold standard treatment for bipolar disorder (BD). However, its mechanism of action incompletely understood, and prediction outcomes limited. In our previous multi-omics study Pharmacogenomics Bipolar Disorder (PGBD) sample combining transcriptomic genomic data, we found that focal adhesion, extracellular matrix (ECM), PI3K-Akt signaling networks were associated with response to lithium. this study, replicated results using network propagation methods in a genome-wide...
Early-life adversities represent risk factors for the development of bipolar affective disorder and are associated with higher severity disorder. This may be consequence a sustained alteration hypothalamic-pituitary-adrenal (HPA) axis resulting from epigenetic modifications gene coding glucocorticoid receptor (NR3C1).To investigate whether childhood maltreatment is increased methylation exon 1F NR3C1 promoter in disorder.A sample people (n = 99) were assessed traumatic experiences. The...
Neuronal excitability has been shown to control the migration and cortical integration of reelin-expressing interneurons (INs) arising from caudal ganglionic eminence (CGE), supporting possibility that neurotransmitters could regulate this process. Here we show ionotropic serotonin receptor 3A (5-HT(3A)R) is specifically expressed in CGE-derived migrating upregulated while they invade developing cortex. Functional investigations using calcium imaging, electrophysiological recordings assays...
Prior research has shown that mothers with Interpersonal violence-related posttraumatic stress disorder (IPV-PTSD) report greater difficulty in parenting their toddlers. Relative to frequent early exposure violence and maltreatment, these display dysregulation of hypothalamic pituitary adrenal axis (HPA-axis), characterized by hypocortisolism. Considering methylation the promoter region glucocorticoid receptor gene NR3C1 as a marker for HPA-axis functioning, less likely being associated...