A5100740812- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA Research and Splicing
- Plant tissue culture and regeneration
- Endoplasmic Reticulum Stress and Disease
- Peroxisome Proliferator-Activated Receptors
- Connexins and lens biology
- Cancer, Hypoxia, and Metabolism
- Natural product bioactivities and synthesis
- Muscle metabolism and nutrition
- Bone Metabolism and Diseases
- Adipose Tissue and Metabolism
- Enzyme Structure and Function
- Retinal Development and Disorders
- Computational Drug Discovery Methods
- Kruppel-like factors research
- Sports Analytics and Performance
- Diet and metabolism studies
- Natural Antidiabetic Agents Studies
- Intraocular Surgery and Lenses
- Advanced Glycation End Products research
- Protein Structure and Dynamics
- Plant Stress Responses and Tolerance
Liaocheng People's Hospital
2016-2025
Beijing Academy of Agricultural and Forestry Sciences
2024
Shaoxing People's Hospital
2022-2023
Penn State Milton S. Hershey Medical Center
2023
Nanfang Hospital
2014-2022
First Affiliated Hospital Zhejiang University
2022
Southern Medical University
2014-2022
Zhejiang University
2022
Pennsylvania State University
2018-2021
Northeast Forestry University
2019-2021
Astragaloside-IV (AS-IV) (C41H68O14) is a high-purity natural product extracted from Astragalus, which has demonstrated biological activities. However, the effect of AS-IV on retinal pigment epithelial (RPE) cells in diabetic retinopathy (DR) remains unclear. In this study, high glucose (HG) was shown to promote ARPE-19 RPE cell death, increase contents reactive oxygen species (ROS) and oxidized glutathione (GSSG), enhance lipid peroxidation density mitochondrial membrane. contrast,...
Scope Quercetin represents antioxidative/antiinflammatory flavonoids widely distributed in the human diet. is efficiently metabolized during absorption to quercetin‐3‐ O ‐glucuronide. This study aims parallelly investigate whether quercetin and ‐glucuronide exert protection against palmitate ( PA )‐induced inflammation insulin resistance endothelium. Methods results Human umbilical vein endothelial cells were pretreated with for 30 min, then incubated 100 μM min or 12 h without insulin....
Long non-coding RNAs (lncRNAs) have recently been identified as critical regulators in tumor initiation and development.However, the function of lncRNAs human hepatocellular carcinoma (HCC) remains largely unknown.Our study was designed to explore biological clinical implication lncRNA MIR22HG HCC.Methods: We evaluated expression 52-patient, 145-patient, TCGA, GSE14520 HCC cohorts.The effects on were analyzed terms proliferation, invasion, metastasis, both vitro vivo.The mechanism action...
Long-term light exposure, especially in the spectrum of blue light, frequently causes excessive oxidative stress dry age-related macular degeneration (AMD). Here, to gain insight into underlying mechanism, we focused on mitochondrial dynamics alterations under long-term exposure mouse and retinal cells. Six-month-old C57BL/6 mice were exposed (450 nm, 800 lx) for 2 weeks. The phenotypic changes retina assayed using haematoxylin-eosin staining transmission electron microscopy. significantly...
Recent studies indicated that long noncoding RNAs (lncRNAs) played important regulatory roles in carcinogenesis and cancer progression.However, the contribution of small nucleolar RNA host gene 20 (SNHG20) to development remains largely unknown.The aim study is investigate expression SNHG20 its clinical significance hepatocellular carcinoma (HCC).Our results showed was remarkably up-regulated HCC tissues compared with adjacent non-tumor liver from 49 fresh samples (cohort 1) detected by...
Abstract The oncogene protein ubiquitin-conjugating enzyme E2T (UBE2T) is reported to be upregulated in hepatocellular carcinoma (HCC) and correlated with poor clinical outcomes of HCC patients. However, the underlying mechanism by which UBE2T exerts its oncogenic function remains largely unexplored. In this study, vitro vivo experiments suggested that promoted development including proliferation metastasis. GSEA analysis indicated was positively pyrimidine metabolism, LC/MS-MS metabolomics...
Pediatric epilepsy presents challenges in treatment optimization, with a significant proportion of patients experiencing inadequate seizure control despite anti-seizure medications (ASMs) therapy. Recent research has indicated the involvement neuroinflammation and immune-mediated mechanisms pathogenesis, suggesting potential interplay between immunological factors ASMs responsiveness. This study aimed to investigate role pediatric generalized, focal their interaction understand influence on...
Fibroblast growth factor 21 (FGF21) is an important regulator of hepatic glucose and lipid metabolism represents a potential pharmacological agent for the treatment type 2 diabetes obesity. Mice fed ketogenic diet (KD) develop insulin resistance in association with high levels FGF21, suggesting state FGF21 resistance. To address role resistance, we assessed action whole-body knock-out (FGF21 KO) male mice their littermate WT controls KD. Here, report that KO have increased production...
Mutations in Dyrk1b are associated with metabolic syndrome and nonalcoholic fatty liver disease humans. Our investigations showed that DYRK1B levels increased the of patients steatohepatitis (NASH) mice fed a high-fat, high-sucrose diet. Increasing mouse enhanced de novo lipogenesis (DNL), acid uptake, triacylglycerol secretion caused NASH hyperlipidemia. Conversely, knockdown was protective against high-calorie-induced hepatic steatosis fibrosis Mechanistically, DNL by activating mTORC2...
We report here the identification and characterization of human mouse <i>PECI</i>, a novel gene that encodes monofunctional peroxisomal Δ<sup>3</sup>,Δ<sup>2</sup>-enoyl-CoA isomerase. Human mouse<i>PECI</i> were identified on basis their sequence similarity to Eci1p, recently characterized isomerase from yeast<i>Saccharomyces cerevisiae</i>. Cloning sequencing <i>PECI</i> cDNA revealed presence 1077-base pair open reading frame predicted encode 359-amino acid protein with mass 39.6 kDa. The...
Talin1 is an adaptor protein that conjugates integrins to the cytoskeleton and regulates focal adhesion signaling. Several studies have found overexpressed in several tumor types promotes progression. However, explicit role of hepatocellular carcinoma ( HCC ) progression still unclear its functional mechanism remains largely unknown. In this study, we showed a trend gradually decreasing expression from normal liver tissues hepatocirrhosis, hyperplasia, corresponding adjacent non‐tumor,...
Abstract Background Dysregulation of long non-coding RNAs (lncRNAs) is responsible for cancer initiation and development, positioning lncRNAs as not only biomarkers but also promising therapeutic targets treatment. A growing number have been reported in hepatocellular carcinoma (HCC), their functional mechanistic roles remain unclear. Methods Gene Set Enrichment Analysis was used to investigate the molecular mechanism UPK1A antisense RNA 1 (UPK1A-AS1). Cell Counting Kit-8 assays, EdU flow...
The degradation of unsaturated fatty acids by β-oxidation involves Δ3,Δ2-enoyl-CoA isomerases (enoyl-CoA isomerases) that catalyze 3-cis → 2-trans and 3-trans isomerizations enoyl-CoAs the 2,5 3,5 isomerization dienoyl-CoAs. An analysis rat liver enoyl-CoA revealed presence a monofunctional isomerase (ECI) in addition to mitochondrial (MECI) mitochondria, whereas peroxisomes contain ECI multifunctional enzyme 1 (MFE1). Thus ECI, which previously had been described as peroxisomal isomerase,...
Ginseng (Panax ginseng C.A. Mey) is known for its rich saponin compounds and tonic effects. To better utilize the medicinal value of ginseng, this study investigated extraction process, components, free radical scavenging ability, immunomodulatory activity total saponins fibrous roots. The response surface methodology was employed to optimize process saponins, Q-Orbitrap high-resolution liquid chromatography–mass spectrometry (LC-MS) used identify chemical constituents in extract roots...
Most inhibitors of Cyclin-dependent kinase 2 (CDK2) target its ATP-binding pocket. It is difficult, however, to use this pocket design very specific because catalytic highly conserved in the protein family CDKs. Here we report some short peptides targeting a noncatalytic near interface CDK2/Cyclin complex. Docking and molecular dynamics simulations were used select peptides, detailed dynamical network analysis revealed that these weaken complex formation via allosteric interactions. Our...
Kakkalide is the predominant isoflavone derived from flowers of Pueraria lobata (Willd.) Ohwi. The aim present study was to investigate effects kakkalide on insulin resistance in endothelium.Human umbilical vein endothelial cells (HUVEC) were stimulated with 100 μmol/L palmitate (PA) for 30 min and pretreatment 0.1-10 reactive oxygen species (ROS)-associated inflammation evaluated by western blot analysis reverse transcription-polymerase chain reaction. Furthermore, we investigated...
Background/Aims: Paclitaxel is largely used as a chemotherapeutic agent for the treatment of several types cancers. However, one significant limiting complications paclitaxel painful peripheral neuropathy during its therapy. The purposes this study were to examine (1) effects blocking mammalian target rapamycin (mTOR) on mechanical and thermal hypersensitivity evoked by paclitaxel; (2) underlying mechanisms responsible role mTOR in regulating paclitaxel-induced neuropathic pain. Methods:...