Gangliosides (GGs), sialic acid-containing glycosphingolipids, are known to be involved in the invasive/metastatic behavior of brain tumor cells. Development modern methods for determination variations GG expression and structure during neoplastic cell transformation is a priority field biomedical analysis. In this context, we report here on first optimization application chip-based nanoelectrospray (NanoMate robot) mass spectrometry (MS) investigation gangliosides secondary tumor. our work...

A sturdy home-built sheathless CE/ESI-QTOF-MS system was developed and optimized for carbohydrate analysis. The interface employed methodology provided a simple analytical solution to laborious CE/MS interfacing methods problems in characterization of complex mixtures that require high-resolution separation the components. CE/ESI interface, feasible any MS laboratory, consists one-piece CE column having terminus in-laboratory shaped as microsprayer coated with copper. inserted into an...

Gangliosides (GGs), a large group of sialylated glycosphingolipids, are considered biomarkers human brain development, aging and certain diseases. Determination individual GG components in complex mixtures extracted from represents fundamental prerequisite for correlating their specificity with the specialized function each area. In context modern glycomics, detailed investigation expression structure requires continuous development application innovative methods able to improve quality data...

We report on a novel strategy for identification of specific sulfation motifs in chondroitin/dermatan sulfate (CS/DS) chain derived from decorin (Dcn), based enzyme cleavage and multistage MS (MS(n)). Released CS/DS chains were digested with chondroitin B parallel AC I lyases to obtain oligosaccharides known hexuronic acid (HexA) epimerization. The depolymerized separated by gel filtration, collected di- hexasaccharides analyzed ESI MS(n). MS(2) bisulfated 4,5-Delta-HexAGalNAc revealed an...

A strategy combining high‐performance thin layer chromatography ( HPTLC ), laser densitometry, and fully automated chip‐based nanoelectrospray (nano ESI chip) performed on a N ano M ate robot coupled to QTOF‐MS was developed, optimized, for the first time applied mapping structural identification of gangliosides (GGs) extracted purified from human angioblastic meningioma specimen. While pattern indicated only seven fractions migrating as GM 3, 2, 1, GD 1a (n LD 1), 1b, GT possibly due high...

Abstract We developed a straightforward approach for high‐throughput top–down glycolipidomics based on fully automated chip‐nanoelectrospray (nanoESI) high‐capacity ion trap (HCT) multistage mass spectrometry (MS n ) by collision‐induced dissociation (CID) in the negative mode. The method was optimized and tested polysialylated ganglioside fraction (GT1b), which profiled MS 1 sequenced tandem up to 6 same experiment. Screening of mode indicated occurrence six [M − 2H] 2− ions which,...

Gangliosides (GGs), sialic acid‐containing glycosphingolipids are involved in many brain functions at the cell and molecular level. Compositional structural elucidation of GGs mixtures extracted from human is essential for correlating their profile with specialized function each area health disease. As a part our ongoing study on GG expression structure different healthy diseased regions, this work, preliminary investigation specimen caudate nucleus (CN) was carried out using an advanced...

Abstract Sulfation pattern within chondroitin sulfate (CS) glycosaminoglycan (GAG) chains is an important post-translational modification that regulates their interaction with proteins. In this context, development of highly efficient and reproducible analytical methods for the investigation CS sulfation patterns high necessity. study we report a novel method straightforward determination N-acetylgalactosamine (GalNAc) sites in disaccharides. Our protocol involves combining fully automated...

Abstract A novel strategy was developed to extend the application of electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) analysis long‐chain polysaccharides. High molecular weight polydisperse maltodextrins (poly‐α(1‐4) glucose) and dextrans (poly‐α(1‐6) were chosen as model compounds in present study. Increased efficiency these mixtures positive mode achieved upon modification their reducing end with nitrogen‐containing groups. The...

Abstract Chondroitin/dermatan sulfate (CS/DS) chain of decorin (DCN) from human skin fibroblasts (HSk) was released by reductive β‐elimination reaction and digested with chondroitin AC I lyase. Enzymatic hydrolysis mixture CS/DS chains separated size‐exclusion chromatography (SEC). Collected octasaccharide fraction subjected to fully automated chip‐based nanoelectrospray (nanoESI) quadrupole time‐of‐flight (QTOF) MS tandem (MS/MS). DCN displayed a high complexity due the large variety...

Abstract 2,5-Furandicarboxylic acid and itaconic are both important biobased platform chemicals their terpolymer with 1,6-hexanediol (HDO) can be the starting point for a new class of reactive polyesters, applications. The green synthetic route developed in this study involves biocatalytic condensation polymerization reaction dimethyl furan-2,5-dicarboxylate (DMFDC) itaconate (DMI) HDO toluene at 80°C, using commercial immobilized lipases from Candida antarctica B. In best conditions, formed...

Glycosphingolipids (GSLs) are a glycolipid subtype which plays vital roles in numerous biological processes, cell–cell interactions, as well oncogenesis and ontogenesis. They ubiquitous molecules found mostly cell membranes. Abnormal expression of GSLs altered molecular structure have been linked with progression cancer metastasis involved the pathophysiology neurodegenerative, autoimmune, infectious diseases inherited enzyme defects—glycosphingolipidoses. Matrix-assisted laser desorption...

Abstract A variety of carbohydrates, in particular polysaccharides can be subjected to chemical modification obtain derivatives with amphiphilic properties, which enable biochemical or biological reactions at the polymer surface. In present work, a polydisperse maltodextrin mixture average molecular weight 3000 was coupled 1,6-hexamethylenediamine (HMD) via reductive amination reaction. Resulting products were characterized by thermal analysis and positive nanoelectrospray quadrupole...