A5016455632- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Renal and related cancers
- Animal Behavior and Welfare Studies
- Veterinary Medicine and Surgery
- Animal Nutrition and Physiology
- Viral Infectious Diseases and Gene Expression in Insects
- Genetic and phenotypic traits in livestock
- Effects of Environmental Stressors on Livestock
- Pregnancy-related medical research
- Microbial Inactivation Methods
- Plant tissue culture and regeneration
- Bee Products Chemical Analysis
- Assisted Reproductive Technology and Twin Pregnancy
- Viral Infections and Immunology Research
- Ovarian function and disorders
- Silk-based biomaterials and applications
- Tissue Engineering and Regenerative Medicine
- CAR-T cell therapy research
- Plant Genetic and Mutation Studies
- Plant Virus Research Studies
Tokushima University
2016-2024
Vietnam National University of Agriculture
2018-2023
Yamaguchi University
2012-2021
A new and highly efficient method for generating mutant pigs by electroporating the CRISPR/Cas9 system into zygotes.
Just one amino acid at the carboxy-terminus of B chain distinguishes human insulin from porcine insulin. By introducing a precise point mutation into (INS) gene, we were able to generate genetically modified pigs that secreted insulin; these may be suitable donors for islet xenotransplantation. The electroporation CRISPR/Cas9 gene-editing system zygotes is frequently used establish rodents, as it requires less time and no micromanipulation. However, has not been point-mutated yet. In present...
Abstract Electroporation is the technique of choice to introduce an exogenous gene into embryos for transgenic animal production. Although this practical and effective, embryonic damage caused by electroporation treatment remains a major problem. This study was conducted evaluate optimal culture system electroporation‐treated porcine supplementation chlorogenic acid ( CGA ), potent antioxidant, during in vitro oocyte maturation. The oocytes were treated with various concentrations (0, 10,...
Abnormal epigenetic reprogramming, such as histone acetylation, might cause low efficiency of interspecies somatic cell nuclear transfer (iSCNT). This study was conducted to evaluate the effects trichostatin A (TSA) on developmental competence and acetylation iSCNT embryos reconstructed from cat cells bovine cytoplasm. The parthenogenetic were treated with various concentrations TSA (0, 25, 50, or 100 nM) for 24 h, respectively, following fusion activation. Treatment 50 nM produced...
The aim of this study was to improve the production efficiency Vietnamese native Ban pig embryos using somatic cell nuclear transfer (SCNT). Fibroblast cells from pigs were injected into enucleated cytoplasts crossbred gilts, and reconstructed subsequently cultured. In first experiment, isolated oocytes matured in either a defined porcine oocyte medium (POM) or TCM199 supplemented with follicular fluid. Both media gonadotropic hormones, for 22 h vitro maturation (IVM) entire 44 IVM. second...
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) technology is growing rapidly and has been greatly influencing the efficiency effectiveness of genetic modifications in different applications. One aspect research gaining importance development system introduction CRISPR materials into target organisms. Although we previously demonstrated efficacy electroporation- lipofection-mediated gene disruption porcine zygotes, still believe that this...
The optimization of single-embryo culture conditions is very important, particularly in the vitro production bovine embryos using ovum pick-up (OPU) procedure. purpose this study was to examine development derived from oocytes obtained by OPU that were cultured either individually or groups medium supplemented with without sericin and investigate viability frozen-thawed after a direct transfer. When two-cell-stage for 7 days CR1aa 0.5% sericin, rates blastocysts freezable significantly lower...
Abstract This study aimed to determine the acetylation at lysine 9/18/23 of histone H3 (H3K9ac/H3K18ac/H3K23ac; H3K9/18/23 ac) and di‐methylation 9 (H3K9me2) during early embryogenesis among trichostatin A (TSA)‐treated interspecies somatic cell nuclear transfer (iSCNT) cat‐cow (TSA‐iSCNT) embryos, TSA‐untreated iSCNT control (control) embryos bovine in vitro fertilization (IVF) because TSA‐iSCNT can develop blastocysts. Compared higher expressions ac were observed IVF most following stages...
The information about risk factors for a high stillbirth rate in piglets is inadequate. Therefore, this study was conducted to determine important an extremely commercial pig farm Vietnam.This included 628 and 45 Landrace × Yorkshire sows. Data including parity number, gestation length (GL), litter size (LS), piglet's gender, stillbirth, birth order, interval (BI), cumulative farrowing duration (CFD), weight (BW), crown-rump (CRL), body mass index, ponderal index (PI) were collected. To deal...
Abstract. Repeat-breeder (RB) cows are a major source of economic waste due to their decreased fertility. Embryo transfer (ET) is an alternative tool improve the fertility RB cows. The aims present study were evaluate effects recipient parity and season on pregnancy rates following ET in Japanese Black beef cattle. Embryos transferred nonsurgically recipients, consisting 155 heifers (< 2 years old) 172 8 old), which defined as Of recipients that presented for ET, 57 received fresh embryo...
Mosaicism, including alleles comprising both wild-type and mutant, is a serious problem for gene modification by editing using electroporation. One-step generation of F0 pigs with completely desired modifications saves cost time, but the major obstacles have been mosaic mutations. We hypothesized that timing electroporation prior to in vitro fertilization (IVF) can increase rates biallelic mutation multiple knockout as permeability mature oocytes greater than zygotes. Hence, we determined...
The oocyte maturation process requires a high supply of energy, which generates reactive oxygen species (ROS), adversely affecting and embryo development. Balancing ROS by antioxidant supplementation is essential for maintaining embryonic quality in vitro. This study aimed to evaluate the impact four antioxidants: β-mercaptoethanol (β-ME), chlorogenic acid (CGA), curcumin sericin, when applied individually or combinations, during on development porcine oocytes. Cumulus-oocyte complexes were...
Interspecies somatic cell nuclear transfer (iSCNT) is an invaluable tool for studying nucleus-cytoplasm interactions and may provide alternative cloning endangered animals whose oocytes are difficult to obtain. The developmental ability of iSCNT embryos decreases with increases in taxonomic distance between the donor recipient species. development cat-bovine reportedly blocked at 8-cell stage (Thongphakdee et al. 2008 J. Reprod. Dev. 54, 142–147). Abnormal epigenetic reprogramming, such as...
Fragmin/protamine microparticles (F/P MPs) approximately 0.5-1 µM in diameter were prepared by the simple mixing of fragmin with protamine. This study investigated effects F/P MP-containing collagen gels as a hormone carrier on formation antral follicle-like structures and development growing bovine oocytes. The supplementation MPs contributed to beneficial follicle stimulating (FSH) size structures. may serve potential carriers for growth cultured oocytes from early follicles.
Follicle-stimulating hormone (FSH) alone can induce oestrus in bitches, but few reports describe oestrous induction by FSH because pregnant mare serum gonadotrophin (PMSG) has been more successful than for induction. Real-time ultrasonography show canine ovarian follicle development, no method determine or predict ovulation accurately. Moreover, the ovary location and size complicate imaging. Using ultrasonography, we investigated treatment stimulation of follicles, with clamping ovaries at...
This study evaluated the effect of dibutyryl cyclic adenosine monophos-phate (dbcAMP) and human chorionic gonadotropin (hCG) on formation antral follicle-like structures (AFLSs) meiotic status bovine cumulus- oocyte complexes (COCs) embedded in collagen gel. Supplementation with dbcAMP increased mean diameter AFLSs during days 4-8 culture compared that control COCs, irrespective concentration used (0.5-2.0 mM). When COCs were cultured for 8 hCG, diameters after 4 tended to be lower...