A5048431264- Preterm Birth and Chorioamnionitis
- Reproductive System and Pregnancy
- Reproductive tract infections research
- Pregnancy and preeclampsia studies
- Pregnancy-related medical research
- Neonatal Respiratory Health Research
- Extracellular vesicles in disease
- Cervical Cancer and HPV Research
- Global Maternal and Child Health
- Data-Driven Disease Surveillance
- Parasites and Host Interactions
- Ovarian cancer diagnosis and treatment
- COVID-19 Impact on Reproduction
- Ethnobotanical and Medicinal Plants Studies
- Moringa oleifera research and applications
- Endometrial and Cervical Cancer Treatments
- Ectopic Pregnancy Diagnosis and Management
- Cardiovascular Issues in Pregnancy
- COVID-19 Clinical Research Studies
- Pelvic floor disorders treatments
- COVID-19 and Mental Health
- Reproductive Health and Contraception
- Global Health and Surgery
- COVID-19 and healthcare impacts
- Hepatitis B Virus Studies
University of the Philippines Manila
2015-2024
De La Salle University
2023-2024
The University of Texas Medical Branch at Galveston
2020-2023
Department of Science and Technology
2022
Osaka Women's and Children's Hospital
2018-2022
Izumi City General Hospital
2019
University of the Philippines Diliman
2015
Objectives: This study documented the species of medicinal plants used by Ayta communities in Dinalupihan, Bataan. The plant parts for purposes, preparations, mode administration these were determined. most important based on use values and informant consensus factors also calculated. Methods: A total 26 informants interviewed regarding they utilize purposes. Free prior informed consents obtained from informants. Taxonomic identification was done Botany Division National Museum Philippines....
Abstract Damage to the cervical epithelial layer due infection and inflammation is associated with preterm birth. However, individual and/or collective roles of layers in maintaining integrity remain unclear during infection/inflammation. To determine intercellular interactions, we developed an organ‐on‐chip (CE‐OOC) composed two co‐culture chambers connected by microchannels, recapitulating ectocervical endocervical layers. Further, tested interactions between cells from each distinct...
Abstract Extracellular vesicles play a crucial role in feto-maternal communication and provide an important paracrine signaling mechanism pregnancy. We hypothesized that fetal cells-derived exosomes microvesicles (MVs) under oxidative stress (OS) carry unique cargo traffic through interface, which cause inflammation uterine cells associated with parturition. Exosomes MVs, from primary amnion epithelial cell (AEC) culture media normal or OS-induced conditions, were isolated by optimized...
Introduction The placenta is essential for fetal growth and survival maintaining a successful pregnancy. sterility of the has been challenged recently; however, presence placental microbiome controversial. We tested hypothesis that bacterial extracellular vesicles (BEVs) from Gram-negative bacteria as an alternate source microbial DNA, regardless existence community in placenta. Methods Placentae term, not labor Cesareans deliveries, were used this study, specimens sampled randomly side....
Background Microbial invasion of the intraamniotic cavity and inflammation are factors associated with spontaneous preterm birth. Understanding route kinetics infection, sites colonization, mechanisms host inflammatory response is critical to reducing birth risk. Objectives This study developed an animal model ascending infection live bacteria ( E . coli ) in pregnant CD-1 mice goal better understanding process microbial inflammation. Study design Multiple experiments were conducted this...
Genital mycoplasmas can break the cervical barrier and cause intraamniotic infection preterm birth. This study developed a six-chamber vagina-cervix-decidua-organ-on-a-chip (VCD-OOC) that recapitulates female reproductive tract during pregnancy with culture chambers populated by vaginal epithelial cells, stromal decidual cells. Cells cultured in VCD-OOC were characterized morphology immunostaining for cell-specific markers. We transferred media from cell chamber of to feto-maternal interface...
Abstract Background Fetal cell-derived exosomes (extracellular vesicles, 40–160 nm) are communication channels that can signal parturition by inducing inflammatory changes in maternal decidua and myometrium. Little is known about their functional roles on the fetal side. This study isolated characterized from decidual myometrial cells grown under normal inflammatory/oxidative stress conditions determined impact membrane cells. Methods Decidual were standard culture (control) or exposed for...
A physiologic increase in reactive oxygen species throughout pregnancy is required to remodel the cervix. Oxidative stress can cause cellular damage that contributes dysfunctional tissue. This study determined oxidative stress-induced cell fate of human cervical epithelial and stromal cells. We treated ectocervical endocervical cells with cigarette smoke extract, an inducer, for 48 h. Cell viability (crystal violet assay); cycle, apoptosis, necrosis (flow cytometry); senescence...
This study determined if exosomes from ectocervical epithelial (ECTO) cells infected with Ureaplasma parvum (U. parvum) can carry bacterial antigens and cause inflammation at the feto-maternal interface using two organ-on-chip devices, one representing vagina-cervix-decidua another mimicking interface, whether such lead to preterm birth (PTB). Exosomes U. -infected ECTO were characterized cryo-electron microscopy, nanoparticle tracking analysis, Western blot, Exoview analysis. The...