A5100368278- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Glioma Diagnosis and Treatment
- Reproductive tract infections research
- Virus-based gene therapy research
- CAR-T cell therapy research
- Preterm Birth and Chorioamnionitis
- Extracellular vesicles in disease
- Bipolar Disorder and Treatment
- Blood groups and transfusion
- Complement system in diseases
- Neurological disorders and treatments
- Cancer Immunotherapy and Biomarkers
- Immunodeficiency and Autoimmune Disorders
- Transcranial Magnetic Stimulation Studies
- Central Venous Catheters and Hemodialysis
- Cancer Research and Treatments
- Reproductive System and Pregnancy
- Patient Satisfaction in Healthcare
- Platelet Disorders and Treatments
- Polyomavirus and related diseases
- Advanced biosensing and bioanalysis techniques
University of California, Davis
2017-2023
University of California Davis Medical Center
2019-2023
Texas A&M University
2021-2022
University of Georgia
2022
Kyungpook National University Hospital
2022
Michigan State University
2012
Kyung Hee University
2005
University of Alabama at Birmingham
1995
CRISPR/Cas9 genome editing is a very promising avenue for the treatment of variety genetic diseases. However, it still challenging to encapsulate machinery delivery. Protein N-myristoylation an irreversible co/post-translational modification that results in covalent attachment myristoyl-group N-terminus target protein. It serves as anchor protein associate with cell membrane and determines its intracellular trafficking activity. Extracellular vesicles (EVs) are secreted mediate cell-cell...
Genital mycoplasmas can break the cervical barrier and cause intraamniotic infection preterm birth. This study developed a six-chamber vagina-cervix-decidua-organ-on-a-chip (VCD-OOC) that recapitulates female reproductive tract during pregnancy with culture chambers populated by vaginal epithelial cells, stromal decidual cells. Cells cultured in VCD-OOC were characterized morphology immunostaining for cell-specific markers. We transferred media from cell chamber of to feto-maternal interface...
Abstract Cross-linking of 4-1BB, a member the TNFR family, increased tyrosine phosphorylation TCR-signaling molecules such as CD3ε, CD3ζ, Lck, linker for activation T cells, and SH2 domain-containing leukocyte phosphoprotein 76 kDa (SLP-76). In addition, incubation activated CD8+ cells with p815 expressing 4-1BBL led to redistribution lipid raft domains protein kinase C-θ, SLP-76, phospholipase C-γ1 (PLC-γ1) on cell membranes areas contact recruitment TNFR-associated factor 2,...
Adaptive human natural killer (NK) cells display significantly enhanced responsiveness to a broad-range of antibody-bound targets through the engagement CD16 compared conventional NK cells, yet direct reactivity against tumor is generally reduced. also distinct phenotype and differential expression numerous genes, including reduced signaling adapter FcRγ transcription factor PLZF. However, it unclear whether specific genes responsible for characteristics adaptive cells. Using CRISPR-Cas9, we...
Human memory-like NK cells are commonly defined by either a lack of FcεRIγ or gain NKG2C expression. Here, we investigated the heterogeneity human CD56dim cell subpopulations according to expression and in large cohort (n=127). Although frequency FcεRIγ– NKG2C+ positively correlated, populations did not exactly overlap. The FcεRIγ+NKG2C+, FcεRIγ–NKG2C+, FcεRIγ–NKG2C–¬ were only evident after HCMV infection, but each had distinct characteristics. Among subpopulations, FcεRIγ–NKG2C+ exhibited...
Abstract “Adaptive” NK cells, characterized by FcRγ deficiency and enhanced responsiveness to Ab-bound, virus-infected have been found in certain hCMV-seropositive individuals. Because humans are exposed numerous microbes environmental agents, specific relationships between hCMV FcRγ-deficient cells (also known as g-NK cells) challenging define. Here, we show that a subgroup of rhesus CMV (RhCMV)-seropositive macaques possesses stably persist display phenotype resembling human cells....
We have investigated the structural determinants of unique functional properties complement factor D by constructing and testing a series trypsin-like mutants enzyme. Mutational replacement primary substrate-binding pocket with that trypsin resulted in mutant (M1) greatly reduced proteolytic activity slightly reactivity toward small thioester substrates. Combining M1 mutations substitution Tyr for Ser94, previously shown to enhance substantially both esterolytic activities D, produced (M2)...
<div>AbstractPurpose:<p>Immune-based therapy for metastatic breast cancer has had limited success, particularly in molecular subtypes with low somatic mutations rates. Strategies to augment T-cell infiltration of tumors include vaccines targeting established oncogenic drivers such as the genomic amplification HER2. We constructed a vaccine based on novel alphaviral vector encoding portion HER2 (VRP-HER2).</p>Patients and Methods:<p>In preclinical studies, mice were...
131 Background: We have previously identified a novel subset of human NK cells that lacks FcRγ, transmembrane signaling adaptor associated with CD16, the Fc receptor for IgG. These FcRγ-deficient (termed g-NK cells) display enhanced functional activity against target cells, including virus-infected in concert target-specific antibody molecules. The presence humans is prior infection by cytomegalovirus (HCMV). Additionally, are activated and expand robustly HCMV-infected HCMV-specific...
Supplementary Figure from A Phase I Study of Autologous Dendritic Cell Vaccine Pulsed with Allogeneic Stem-like Line Lysate in Patients Newly Diagnosed or Recurrent Glioblastoma
<div>AbstractPurpose:<p>Glioblastoma (GBM) is a heterogeneous malignancy with multiple subpopulations of cancer cells present within any tumor. We the results phase I clinical trial using an autologous dendritic cell (DC) vaccine pulsed lysate derived from GBM stem-like line.</p>Patients and Methods:<p>Patients newly diagnosed recurrent were enrolled as separate cohorts. Eligibility criteria included qualifying surgical resection or minimal tumor size, ≤ 4-mg...
Supplementary Figure from A Phase I Study of Autologous Dendritic Cell Vaccine Pulsed with Allogeneic Stem-like Line Lysate in Patients Newly Diagnosed or Recurrent Glioblastoma
Supplementary Data from A Phase I Study of Autologous Dendritic Cell Vaccine Pulsed with Allogeneic Stem-like Line Lysate in Patients Newly Diagnosed or Recurrent Glioblastoma
Supplementary Data from A Phase I Study of Autologous Dendritic Cell Vaccine Pulsed with Allogeneic Stem-like Line Lysate in Patients Newly Diagnosed or Recurrent Glioblastoma
<div>AbstractPurpose:<p>Glioblastoma (GBM) is a heterogeneous malignancy with multiple subpopulations of cancer cells present within any tumor. We the results phase I clinical trial using an autologous dendritic cell (DC) vaccine pulsed lysate derived from GBM stem-like line.</p>Patients and Methods:<p>Patients newly diagnosed recurrent were enrolled as separate cohorts. Eligibility criteria included qualifying surgical resection or minimal tumor size, ≤ 4-mg...
Elderly people are at greater risk for complications from viral infections. A common explanation is that elderly have compromised immunity. Indeed, conservation of good natural killer (NK) cell cytotoxicity has been correlated with increased survival in later stages life. Our objective to examine how ageing affects NK homeostasis and their responses specific stimuli. We use flow cytometric methods the differences among cells adult (6 month) aged (22 month), male, C57/Bl6 mice. Herein, we...
BackgroundNatural killer (NK) cells are a part of the innate immune system and play major role in immunity against viral infections malignancies. Recently new subset NK has been discovered that lacks FcRg transmembrane signaling adaptor. These FcRg-deficient have termed FcR gamma-negative or g-NK cells. The display robust reaction CMV other presence virus-specific antibodies.Study ObjectivesA longitudinal, prospective study is being conducted to observe generation allogeneic hematopoietic...
Abstract Background A central venous catheter (CVC) is an important medical device, but it could be preceding infection and the risk of line-associated bloodstream (CLABSI). CLABSI a common healthcare-associated results in high cost mortality; therefore, various efforts to reduce have been attempted. Methods We reviewed analysed data rates days from insertion removal temporary between January 2018 June 2021 intensive care unit (ICU) single tertiary hospital. Sequentially, all patients with...
Abstract Long-lived ‘memory-like’ NK cells, characterized by FcRγ-deficiency and enhanced responsiveness to antibody-bound virus-infected have been found in certain human cytomegalovirus (HCMV)-seropositive individuals. Because humans are exposed numerous microbes environmental agents, specific relationships between HCMV FcRγ-deficient cells (also known as g-NK cells) challenging define. Here, we show that a subgroup of rhesus (RhCMV)-seropositive macaques possesses stably persist display...