A5100422850- Glaucoma and retinal disorders
- Extracellular vesicles in disease
- Corneal surgery and disorders
- Retinal Diseases and Treatments
- Corneal Surgery and Treatments
- MicroRNA in disease regulation
- Lung Cancer Treatments and Mutations
- Retinal Development and Disorders
- Parkinson's Disease Mechanisms and Treatments
- Ocular Surface and Contact Lens
- Lung Cancer Diagnosis and Treatment
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Lung Cancer Research Studies
- Retinal Imaging and Analysis
- Proteoglycans and glycosaminoglycans research
- Ophthalmology and Eye Disorders
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Connexins and lens biology
- RNA regulation and disease
- Tissue Engineering and Regenerative Medicine
- Genetics and Neurodevelopmental Disorders
Augusta University
2016-2025
Discovery Institute
2016-2025
Augusta University Health
2016-2025
Nankai University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2024
First Affiliated Hospital of Zhengzhou University
2015-2024
Air Force Engineering University
2024
Georgia Regents Medical Center
2014-2023
Chengdu Neusoft University
2023
Zhuhai People's Hospital
2023
Exosomes play a role in cell-to-cell signaling and serve as possible biomarkers. Isolating exosomes with reliable quality substantial concentration is major challenge. Our purpose to compare the extracted by three different exosome isolation kits (miRCURY, ExoQuick, Invitrogen Total Exosome Isolation Reagent) differential ultracentrifugation (UC) using six volumes of non-cancerous human serum (5 ml, 1 500 μl, 250 100 50 μl) (1 μl individual commercial samples collected from donors. The...
Recent evidence implicates exosomes in the aggregation of Aβ and spreading tau Alzheimer9s disease. In neural cells, exosome formation can be blocked by inhibition or silencing neutral sphingomyelinase-2 (nSMase2). We generated genetically nSMase2-deficient 5XFAD mice (<i>fro;</i>5XFAD) to assess AD-related pathology a mouse model with consistently reduced ceramide generation. conducted <i>in vitro</i> assays Aβ<sub>42</sub> glial clearance without isolated ultracentrifugation determined...
Microvesicle- and exosome-mediated transport of microRNAs (miRNAs) represents a novel cellular molecular pathway for cell-cell communication. In this study, we tested the hypothesis that these extracellular vesicles (EVs) their miRNAs might change with age, contributing to age-related stem cell dysfunction. EVs were isolated from bone marrow interstitial fluid (supernatant) young (3-4 months) aged (24-28 mice determine whether size, concentration, miRNA profile altered age in vivo. Results...
The pathogenesis of osteoarthritis (OA) is poorly understood, and therapeutic approaches are limited to preventing progression the disease. Recent studies have shown that exosomes play a vital role in cell-to-cell communication, many age-related diseases. Molecular profiling synovial fluid derived exosomal miRNAs may increase our understanding OA lead discovery novel biomarkers targets. In this article we report first characterization from human fluid. share similar characteristics (size,...
Extracellular vesicles (EVs) are known to play important roles in cell-cell communication.Here we investigated the role of muscle-derived EVs and their microRNAs loss bone stem cell populations with age.Aging male female C57BL6 mice was associated a significant increase expression senescenceassociated microRNA miR-34a-5p (miR-34a) skeletal muscle serum -derived EVs.Muscle-derived, alpha-sarcoglycan positive, isolated from samples also showed miR-34a age.EVs were conditioned medium C2C12...
Abstract A general strategy for the preparation of highly fluorescent poly(DL‐lactide‐ co ‐glycolide) (PLGA) nanoparticles (NPs) loaded with conjugated polymers (CPs) is reported. The process involves encapsulation organic‐soluble CPs PLGA using a modified solvent extraction/evaporation technique. obtained NPs are stable in aqueous media biocompatible and functionalizable surfaces. In addition, properties CP‐loaded (CPL NPs) could be fine‐tuned by loading different types into matrix. Four...
Exosomes are nano-sized vesicles produced and secreted by cells to mediate intercellular communication. The production function of exosomes in kidney tissues remain largely unclear. Hypoxia is a common pathophysiological condition kidneys. This study was designed characterize exosome during hypoxia rat renal proximal tubular (RPTCs), investigate the regulation hypoxia-inducible factor-1 (HIF-1), determine effect on ATP-depletion-induced cell injury. did not change average sizes RPTCs, but it...
Primary open angle glaucoma (POAG), a major cause of blindness worldwide, is complex disease with significant genetic contribution. We performed Exome Array (Illumina) analysis on 3504 POAG cases and 9746 controls replication the most findings in 9173 26 780 across 18 collections Asian, African European descent. Apart from confirming strong evidence association at CDKN2B-AS1 (rs2157719 [G], odds ratio [OR] = 0.71, P 2.81 × 10−33), we observed one SNP showing to (CDC7–TGFBR3 rs1192415,...