A5086342364- Helicobacter pylori-related gastroenterology studies
- Berry genetics and cultivation research
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- Galectins and Cancer Biology
- Microbial Metabolism and Applications
- Plant Physiology and Cultivation Studies
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Escherichia coli research studies
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Gastroesophageal reflux and treatments
- Toxin Mechanisms and Immunotoxins
- Horticultural and Viticultural Research
- Gastrointestinal motility and disorders
- Enzyme Structure and Function
- Biochemical and Structural Characterization
- Weed Control and Herbicide Applications
- Microbial Metabolites in Food Biotechnology
- Eosinophilic Esophagitis
- Antibiotic Resistance in Bacteria
- Peptidase Inhibition and Analysis
- Freezing and Crystallization Processes
- Esophageal and GI Pathology
Imperial College London
2015-2022
Medical Research Council
2016-2021
Institut de Biologie Structurale
2011-2014
Centre National de la Recherche Scientifique
2011-2014
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2011-2014
Université Grenoble Alpes
2011-2014
CEA Grenoble
2013-2014
Université Joseph Fourier
2011
European Synchrotron Radiation Facility
2009
European Science Foundation
2009
Nucleotide signaling molecules are important intracellular messengers that regulate a wide range of biological functions. The human pathogen Staphylococcus aureus produces the nucleotide cyclic di-AMP (c-di-AMP). This molecule is common among Gram-positive bacteria and in many organisms essential for survival under standard laboratory growth conditions. In this study, we investigated interaction c-di-AMP with S. KdpD protein. sensor kinase forms two-component system response regulator KdpE...
Infection with the gastric pathogen Helicobacter pylori is a risk factor for development of cancer. Pathogenic strains H. carry type IV secretion system (T4SS) responsible injection oncoprotein CagA into host cells. and its cag-T4SS exploit α5β1 integrin as receptor translocation. Injected localizes to inner leaflet cell membrane, where it hijacks signaling induces cytoskeleton reorganization. Here we describe crystal structure N-terminal ~100-kDa subdomain at 3.6 Å that unveils unique...
The second messenger c-di-AMP binds to and inhibits a component of transporter that helps bacterial cells adapt osmotic stress.
c-di-AMP is an important second messenger molecule that plays a pivotal role in regulating fundamental cellular processes, including osmotic and cell wall homeostasis many Gram-positive organisms. In the opportunistic human pathogen Staphylococcus aureus, produced by membrane-anchored DacA enzyme. Inactivation of this enzyme leads to growth arrest under standard laboratory conditions re-sensitization methicillin-resistant S. aureus (MRSA) strains ß-lactam antibiotics. The gene coding for...
Lipoteichoic acid synthase (LtaS) is a key enzyme for the cell wall biosynthesis of Gram-positive bacteria. bacteria that lack lipoteichoic (LTA) exhibit impaired division and growth defects. Thus, LtaS appears to be an attractive antimicrobial target. The pharmacology around remains largely unexplored with only two small-molecule inhibitors reported, namely "compound 1771" Congo red dye. Structure-based drug discovery efforts against remain unattempted due inhibitor-bound structure LtaS. To...
Summary A crucial aspect of the functionality bacterial type II secretion systems is targeting and assembly outer membrane secretin. In Klebsiella oxytoca system, lipoprotein PulS, a pilotin, targets secretin PulD monomers through periplasm to membrane. We present crystal structure an all‐helical bundle that structurally distinct from proteins with similar functions. Replacement valine at position 42 in charged groove PulS abolished complex formation between non‐lipidated variant peptide...
Interaction of bacterial outer membrane secretin PulD with its dedicated lipoprotein chaperone PulS relies on a disorder-to-order transition the binding (S) domain near C terminus. interacts purified S to form 1:1 complex. Circular dichroism, one-dimensional NMR, and hydrodynamic measurements indicate that is elongated intrinsically disordered but gains secondary structure upon PulS. Limited proteolysis mass spectrometry identified 28 C-terminal residues as minimal site low nanomolar...
Highly pathogenic strains of Helicobacter pylori use a type IV secretion system to inject the CagA protein into human gastric cells. There, associates with inner side membrane and is tyrosine-phosphorylated at EPIYA motifs by host kinases. The phosphorylation triggers series interactions between proteins that result in dramatic change cellular morphology. Structural functional analyses have proved difficult, due proteolytically sensitive nature recombinant protein. To circumvent these...
TIP alpha (uniprotkb:B2UTN0) and bind (MI:0407) by cosedimentation (MI:0027)
Cyclic-di-adenosine monophosphate (c-di-AMP) is an important nucleotide signaling molecule that plays a key role in osmotic regulation bacteria. c-di-AMP produced from two molecules of ATP by proteins containing diadenylate cyclase (DAC) domain. In Bacillus subtilis, the main cyclase, CdaA, membrane-linked with N-terminal transmembrane domain followed cytoplasmic DAC As both high and low levels have negative impact on bacterial growth, cellular this are tightly regulated. Here we...
SummaryPaclobutrazol was applied as a foliar spray on strawberry plants cv Belrubi, grown under tunnels, before flower emergence at rates equivalent to 6.75, 3.37,1.68, 0.84, 0.42, 0.21 and 0.10 kg ha−1, with without surfactant. The chemical did not affect yield, but plant growth greatly reduced all application rates. Petioles were shortened, the leaves appeared tightly adpressed crown, leaf surface runner formation inhibited. also affected dry weight partitioning within plant,...
Cell-free extract and purified enzyme-based systems provide an attractive solution to study biosynthetic strategies towards a range of chemicals. 4-(4-hydroxyphenyl)-butan-2-one, also known as raspberry ketone, is the major fragrance component fruit used natural additive in food sports industry. Current industrial processing form ketone involves chemical extraction from yield ∼1-4 mg kg-1 fruit. Due toxicity, microbial production provides only low yields up 5-100 L-1. Herein, we report...
ABSTRACT Background Available data conflict regarding the possible relation between chronic gastritis, Helicohacter pylori (H p), and gastric motor disorders in nonulcer dyspepsia. The aim of this study, therefore, was (1) evaluate both gastroduodenal fasting motility emptying subjects with functional dyspepsia, without (2) to correlate pattern H p infection. Materials Methods. Thirty‐eight patients were studied, 20 positive for Hp infection (15 gastritis) 18 Hp‐negative (8 gastritis). All...
Helicobacter pylori chronically infects the gastric mucosa of millions people annually worldwide: it has been estimated that over 50% world population carries this infection. H. associated with development several diseases, like chronic gastritis, and duodenal ulcer, adenocarcinoma mucosa-associated lymphoma [1-3]. The complete genome sequence two different isolates (J99 26995) is known. The strains contain a 37 kb foreign DNA region, called cag pathogenicity island (cag-PAI), cause most...