A5038965467- Microbial Metabolites in Food Biotechnology
- Enzyme Structure and Function
- Protein Hydrolysis and Bioactive Peptides
- Helicobacter pylori-related gastroenterology studies
- Enzyme Production and Characterization
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- Probiotics and Fermented Foods
- Biochemical and Structural Characterization
- Glycosylation and Glycoproteins Research
- Brucella: diagnosis, epidemiology, treatment
- Galectins and Cancer Biology
- Plant Reproductive Biology
- Ultrasound and Cavitation Phenomena
- Microbial Inactivation Methods
- Monoclonal and Polyclonal Antibodies Research
- Food composition and properties
- Collagen: Extraction and Characterization
- Plant nutrient uptake and metabolism
- Plant Micronutrient Interactions and Effects
- Transgenic Plants and Applications
- Fungal and yeast genetics research
- Escherichia coli research studies
- Microfluidic and Bio-sensing Technologies
- Consumer Attitudes and Food Labeling
Ege University
2017-2023
Sabancı Üniversitesi
2009-2020
Université Claude Bernard Lyon 1
2012-2014
Institut de Biologie et de Chimie des Protéines
2013-2014
Centre National de la Recherche Scientifique
2013-2014
Structural and Molecular Basis of Infectious Systems
2014
Infection with the gastric pathogen Helicobacter pylori is a risk factor for development of cancer. Pathogenic strains H. carry type IV secretion system (T4SS) responsible injection oncoprotein CagA into host cells. and its cag-T4SS exploit α5β1 integrin as receptor translocation. Injected localizes to inner leaflet cell membrane, where it hijacks signaling induces cytoskeleton reorganization. Here we describe crystal structure N-terminal ~100-kDa subdomain at 3.6 Å that unveils unique...
X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this to unravel mechanistic details biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid or large compartments). Since its inception, single-crystal cryocrystallography has never been performed in Türkiye due the lack diffractometer. The diffraction facility recently established at...
BtpA/Btp1/TcpB is a virulence factor produced by Brucella species that possesses Toll interleukin‐1 receptor (TIR) domain. Once delivered into the host cell, BtpA interacts with MyD88 to interfere TLR signalling and modulates microtubule dynamics. Here crystal structure of TIR domain at 3.15 Å presented. The shows dimeric arrangement canonical domain, similar Paracoccus denitrificans Tir protein but secured unique long N‐terminal α‐tail packs against TIR:TIR dimer. Structure‐based mutations...
The TIR domain-containing proteins BtpA/Btp1/TcpB and BtpB are translocated into host cells by the facultative intracellular bacterial pathogen Brucella. Here, they interfere with Toll like receptor signalling to temper inflammatory response. BtpA has also been found modulate microtubule dynamics. In both we identified a WxxxE motif, previously shown be an essential structural component in family of type III secretion system effectors that actin dynamics functioning as guanine nucleotide...
Abstract This study aimed to extract collagen‐I from lamb feet (LF) and examine the effects of ultrasound treatment on structural molecular characteristics collagen. Compared ultrasonic bath conventional extraction methods, probe (USP) significantly increased collagen content ( p < 0.05). The electrophoretic profiles confirmed presence α‐ β‐chains, indicating it as type I. Furthermore, X‐ray diffraction, Fourier‐transform infrared spectroscopy, circular dichroism spectra analyses revealed...
Abstract High-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography an emerging structural biology technique which has fundamental limitations due either sample volume requirements or immediate access the competitive X-ray beamtime. Obtaining a high of well-diffracting, sufficient-size crystals while mitigating radiation damage remains critical bottleneck serial crystallography. As alternative, we...
This article investigates the production and optimization of levansucrase using synthetic medium by Zymomonas mobilis NRRL B-14023. Response surface methodology was used to investigate effects three different fermentation parameters on enzyme production. Maximum activity 13.3 µmol glucose/min obtained at optimum levels process variables (pH 4.90, initial sucrose concentration 159.01 g/L temperature 30.3°C). found be useful in optimizing determining interactions among variables. The purity...
Levansucrases are bacterial enzymes which produce fructan polymers from sucrose via hydrolysis and transfructosylation activities.These polymers; levan fructooligosaccharides valuable for food pharmaceutical industries.Levansucrases Gram-positive bacteria such as Bacillus subtilis tend to levan, while those Gram-negative preferentially fructooligosaccharides.Zymomonas mobilis is an efficient levansucrase producer its extracellular can both depending on the reaction parameters.In this study,...
ABSTRACT High-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography an emerging structural biology technique which has fundamental limitations due either sample volume requirements or immediate access the competitive X-ray beamtime. Obtaining a high of well-diffracting, sufficient-size crystals while mitigating radiation damage remains critical bottleneck serial crystallography. As alternative, we...
Abstract X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this to unravel mechanistic details biological macromolecules (e.g. proteins, nucleic acids, protein complexes, protein-nucleic acid or large compartments). Since its inception, single-crystal cryo-crystallography has never been performed in Türkiye due the lack diffractometer. The diffraction facility recently established...
In this initial study, the effect of hydrodynamic flow on lysozyme structure and function was investigated using a microchannel device. Protein subjected to bubbly cavitation as well noncavitating conditions at pH 4.8 25 °C. Interestingly, time course analyses indicated that secondary content, diameter, enzymatic activity were unaffected by cavitation. However, did induce decrease diameter. The corresponding structural change subtle extent bioactivity marginally suppressed. Moreover, native...
Probiyotikler yeterli miktarda tüketildiklerinde sağlık üzerine yararlı etkiler gösteren canlı mikroorganizmalardır. Bu mikroorganizmalar, patojenlerle mücadele, epitel hücre kararlılığını sağlama ve bağışıklık sistemini düzenleyebilmeleri ile olumlu göstermektedirler.Bağışıklık düzenleyici, anti-inflamatuar, anti-mikrobiyal, anti-oksidan etkilerini, açığa çıkardıkları metabolitleriyle, ürettikleri moleküllerle
Probiyotiklerin gıda sektöründe kullanımı oldukça yaygındır, buna rağmen gıdalara canlı mikroorganizma eklenmesi ile ilgili teknolojik problemler ve sağlık riskleri tartışmalar devam etmektedir. Son yıllarda probiyotikler yerine postbiyotiklerin gıdalarda çalışmalar hızla artmaktadır. Postbiyotikler, tarafından üretilen ekzopolisakkaritler, kısa zincirli yağ asitleri, enzimler, peptitler, bakteriyosinler, vitaminler, plazmalojenler, peptidoglikanlar gibi moleküllerdir. Gıdalara eklenmesiyle,...
Günümüzde, beslenme alışkanlıkları ile insan sağlığı arasındaki ilişkinin ortaya konulması özellikle gıda kaynaklı biyoaktif bileşenleri hedef alan çalışmaların giderek artmasına sebep olmaktadır. Gıda peptitler ise sahip oldukları potansiyel biyoaktivite araştırmacılar için oldukça önemli bir olarak çıkmıştır. Biyoaktif peptitler, birçok sağlık etkisine olan spesifik amino asit dizileridir ve sindirim enzimleri, proteolitik enzimler veya fermantasyon sonucu gerçekleşen protein hidrolizi...
Helicobacter pylori, an important human pathogen, is capable of causing persistent infection with minimal immune response. The first line defense during H. pylori through gastric epithelial cells that present TLR, A family bacterial proteins share homology the Toll/IL-1 receptor (TIR) domain were identified. Bacterial TIR (BTP) mimic and act on myeloid differentiation primary response gene 88 (MyD88) signaling pathways to suppress TLR signaling. may also produce a similar protein. putative...
Levan gıda, kozmetik ve ilaç sanayi gibi birbirinden farklı endüstrilerde kullanım alanına sahip bir fruktoz polimeridir. Levansukrazlar sakkarozu substrat olarak kullanarak polimerleri oluşumunu katalizleyen enzimlerdir. Bakteriler tarafından hücre dışına salgılanan levansukrazlar bakterinin yüksek sakkaroz konsantrasyonunda levan ve/veya fruktooligosakkarit polimerlerini sentezlemesini sağlar. Bu çalışmada Zymomonas mobilis NRRL B-14023 kullanılarak levansukraz enziminin üretilmesi...
There is a growing interest and increasing awareness of consumers towards natural food products, therefore there shift in industry to produce foods with ingredients. On the other hand, high amount sweetener use another health concern. An interesting group sweeteners are sweet proteins, which have hundreds/thousands times higher sweetness than sucrose. Sweet proteins but low calorie values as they can be used healthy alternatives or artificial sweeteners. Known plant produced by tropical...