A5090655405- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Viral Infectious Diseases and Gene Expression in Insects
- Bioinformatics and Genomic Networks
- Algal biology and biofuel production
- Microbial Community Ecology and Physiology
- Photosynthetic Processes and Mechanisms
- Pancreatic function and diabetes
- Creativity in Education and Neuroscience
- Global Energy and Sustainability Research
- 3D Printing in Biomedical Research
- Transgenic Plants and Applications
- Science Education and Perceptions
- Toxin Mechanisms and Immunotoxins
- Collagen: Extraction and Characterization
- RNA modifications and cancer
- Microbial metabolism and enzyme function
- Electrospun Nanofibers in Biomedical Applications
- Protist diversity and phylogeny
- RNA and protein synthesis mechanisms
- Microbial Fuel Cells and Bioremediation
- Environmental Impact and Sustainability
- Silk-based biomaterials and applications
Austrian Centre of Industrial Biotechnology (Austria)
2020-2025
BOKU University
2017-2024
Institute of Molecular Biotechnology
2024
Middle East Technical University
2012-2017
Bioengineering Center
2017
National Center for Genetic Engineering and Biotechnology
2017
John Wiley & Sons (United States)
2017
Hudson Institute
2017
The increase of CO 2 emissions due to human activity is one the preeminent reasons for present climate crisis. In addition, considering increasing demand renewable resources, upcycling as a feedstock gains an extensive importance establish -neutral or -negative industrial processes independent agricultural resources. Here we assess whether synthetic autotrophic Komagataella phaffii ( Pichia pastoris ) can be used platform value-added chemicals using by integrating heterologous genes lactic...
ABSTRACT The constitutive glyceraldehyde‐3‐phosphate dehydrogenase promoter (P GAP ), which is one of the benchmark promoters Pichia pastoris , was analyzed in terms putative transcription factor binding sites. We constructed a synthetic library with distinct regulatory properties through deletion and duplication these sites selected (TF) genes were overexpressed or deleted to understand their roles on heterologous protein production. Using enhanced green fluorescent protein, an expression...
Abstract The Crabtree phenotype defines whether a yeast can perform simultaneous respiration and fermentation under aerobic conditions at high growth rates. It provides positive yeasts an evolutionary advantage of consuming glucose faster producing ethanol to outcompete other microorganisms in sugar rich environments. While number genetic events are associated with the emergence effect, its evolution remains unresolved. Here we show that overexpression single Gal4-like transcription factor...
Increasing atmospheric carbon dioxide levels, a reduction of arable land area and the dependence first second generation biotechnology feedstocks on agricultural products, call for alternative, sustainable feedstock sources industrial applications. The direct use CO2 or conversion into other single (C1) have great potential as they might help to reduce emissions do not compete with use. Here we discuss microbial C1 sources, their applications in biotechnology, challenges towards C1-based...
Yeasts and especially Pichia pastoris (syn Komagataella spp.) are popular microbial expression systems for the production of recombinant proteins. One key advantages yeast host is their ability to secrete protein into culture media. However, secretion some proteins less efficient. These include antibody fragments such as Fabs or scFvs. We have recently identified translocation nascent Fab from cytosol endoplasmic reticulum (ER) one major bottleneck. Conceptually, this bottleneck requires...
Abstract Single carbon (C1) molecules are considered as valuable substrates for biotechnology, they serve intermediates of dioxide recycling, and enable bio-based production a plethora substances our daily use without relying on agricultural plant production. Yeasts chassis organisms biotech production, able to C1 either natively or synthetic engineered strains. This review highlights native yeast pathways methanol formate assimilation, their engineering, the realization heterologous...
Amidst the escalating carbon dioxide levels resulting from fossil fuel consumption, there is a pressing need for sustainable, bio-based alternatives to underpin future global economies. Single-carbon feedstocks, derived CO
ABSTRACT BACKGROUND The effects of designed methanol and co‐substrate feeding strategies on fed‐batch recombinant human growth hormone ( rhGH ) production by Pichia pastoris hGH ‐Mut + were investigated. RESULTS highest cell concentration was obtained as 157 g L −1 with the exponential strategy where fed at a pre‐calculated rate based pre‐determined µ 0 = 0.03 h , mannitol kept constant 50 for t =0–6 h. 1.3 =54 methanol‐stat strategy, no used 5 in medium during phase. Thereafter, another...
Microbial metabolism has been deeply studied over decades and it is considered to be understood a great extent. Annotated genome sequences of many microbial species have contributed lot generating biochemical knowledge on metabolism. However, researchers still discover novel pathways, unforeseen reactions or unexpected metabolites which contradict the expected canon in living organisms. Here, we highlight few examples such non-canonical how they were found, what their importance...
Abstract A yeast deletion mutation in the nuclear-encoded gene, AFO1, which codes for a mitochondrial ribosomal protein, led to slow growth on glucose, inability grow glycerol or ethanol, and loss of DNA respiration. We noticed that afo1- readily obtains secondary mutations suppress aspects this phenotype, including its defect. characterized identified dominant missense suppressor ATP3 gene. Comparing isogenic slowly growing rho-zero rapidly suppressed strains under carefully controlled...
Abstract BACKGROUND Biochemical reaction network of Pichia pastoris was improved by including N ‐ glycosylation pathway reactions to determine the intracellular rates for glycosylated protein production. RESULTS When co‐substrate sorbitol replaced with mannitol, 12‐fold increase in erythropoietin flux obtained as a result 1.2‐ and 2.4‐fold glucose‐6‐phosphate (G6P) formation from fructose‐6‐phosphate (F6P) 3‐phospho‐D‐glycerate (3PG) glyceraldehyde‐3‐phosphate (G3P), respectively. Thus,...
In this article we explore the intersection of science and art through a collaboration between us scientists bioartists Anna Dimitriu Alex May, focusing on interface yeast biotechnology art. The collaboration, originally initiated in 2018, resulted three major artworks: CULTURE, depicting evolution human societies; FERMENTING FUTURES, illustrating synthetic autotrophic its link to lactic acid production; WOOD SPIRIT-AMBER ACID, inspired by VIVALDI project targeting CO2 reduction methanol. We...
Abstract Background Amidst the escalating carbon dioxide levels resulting from fossil fuel consumption, there is a pressing need for sustainable, bio-based alternatives to underpin future global economies. Single feedstocks, derived CO 2 , represent promising substrates biotechnological applications. Especially methanol gaining prominence bio-production of commodity chemicals. Results In this study, we show potential Komagataella phaffii as production platform itaconic acid using source....
Abstract Synthetic autotrophs can serve as chassis strains for bioproduction from CO 2 a feedstock to take measures against the climate crisis. Integration of Calvin–Benson–Bassham (CBB) cycle into methylotrophic yeast Komagataella phaffii ( Pichia pastoris ) enabled it use sole carbon source. The key enzyme in this is ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzing carboxylation step. However, error prone perform an oxygenation reaction leading production toxic...
Abstract Synthetic autotrophs can serve as chassis strains for bioproduction from CO2 a feedstock to take measures against the climate crisis. Integration of Calvin–Benson–Bassham (CBB) cycle into methylotrophic yeast Komagataella phaffii (Pichia pastoris) enabled it use sole carbon source. The key enzyme in this is ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzing carboxylation step. However, error prone perform an oxygenation reaction leading production toxic...
BioArt is a new discipline where artists employ materials and techniques of modern life sciences create novel meanings biology, often involving living organisms such as tissue culture, bacteria yeasts, which may also be genetically engineered. The authors have engaged in collaboration to develop 'Fermenting Futures', project designed explore the significance yeast for early human history by enabling baking brewing, all way industrial biotechnology synthetic biology with their potential...