A5041718604- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Microbial Metabolic Engineering and Bioproduction
- Advanced Proteomics Techniques and Applications
- Pesticide Residue Analysis and Safety
- Isotope Analysis in Ecology
- Soil Carbon and Nitrogen Dynamics
- Protist diversity and phylogeny
- Advanced Chemical Sensor Technologies
- Fungal and yeast genetics research
- Plant nutrient uptake and metabolism
- Meat and Animal Product Quality
- Agronomic Practices and Intercropping Systems
- Pharmaceutical and Antibiotic Environmental Impacts
- Genetics, Aging, and Longevity in Model Organisms
- Agriculture, Plant Science, Crop Management
- Biosensors and Analytical Detection
- Agricultural Science and Fertilization
- Advanced Biosensing Techniques and Applications
- Analytical chemistry methods development
- Bioinformatics and Genomic Networks
- Legume Nitrogen Fixing Symbiosis
- Yeasts and Rust Fungi Studies
- Chromatography in Natural Products
BOKU University
2015-2024
University of Vienna
2024
Swiss Federal Institute of Aquatic Science and Technology
2020-2021
Austrian Centre of Industrial Biotechnology (Austria)
2015-2018
Collision cross section (CCS) measurements resulting from ion mobility–mass spectrometry (IM-MS) experiments provide a promising orthogonal dimension of structural information in MS-based analytical separations. As with any molecular identifier, interlaboratory standardization must precede broad range integration into workflows. In this study, we present reference drift tube mobility mass spectrometer (DTIM-MS) where improvements on the measurement accuracy experimental parameters...
Abstract Increasing production and use of chemicals awareness their impact on ecosystems humans has led to large interest for broadening the knowledge chemical status environment human health by suspect non-target screening (NTS). To facilitate effective implementation NTS in scientific, commercial governmental laboratories, as well acceptance managers, regulators risk assessors, more harmonisation is required. address this, NORMAN Association members involved activities have prepared this...
Deciphering root exudate composition of soil-grown plants is considered a crucial step to better understand plant-soil-microbe interactions affecting plant growth performance. In this study, two genotypes Zea mays L. (WT, rth3) differing in hair elongation were grown the field substrates (sand, loam) custom-made, perforated columns inserted into plots. Root exudates collected at different developmental stages (BBCH 14, 19, 59, 83) using soil-hydroponic-hybrid exudation sampling approach....
Abstract Roots are crucial in plant adaptation through the exudation of various compounds which influenced and modified by environmental factors. Buckwheat root exudate system response to neighbouring plants (buckwheat or redroot pigweed) how these exudates affect pigweed was investigated. Characterising plant–plant interactions presents challenges, therefore a split-root enabled application differential treatments parts single non-destructive sampling developed. Non-targeted metabolome...
Nontargeted screening (NTS) utilizing liquid chromatography electrospray ionization high-resolution mass spectrometry (LC/ESI/HRMS) is increasingly used to identify environmental contaminants. Major differences in the efficiency of compounds ESI/HRMS result widely varying responses and complicate quantitative analysis. Despite an increasing number methods for quantification without authentic standards NTS, approaches are evaluated on limited diverse data sets with chemical coverage collected...
Abstract The Crabtree phenotype defines whether a yeast can perform simultaneous respiration and fermentation under aerobic conditions at high growth rates. It provides positive yeasts an evolutionary advantage of consuming glucose faster producing ethanol to outcompete other microorganisms in sugar rich environments. While number genetic events are associated with the emergence effect, its evolution remains unresolved. Here we show that overexpression single Gal4-like transcription factor...
Isotope tracer experiments are an invaluable technique to analyze and study the metabolism of biological systems. However, isotope labeling often affected by naturally abundant isotopes especially in cases where mass spectrometric methods make use derivatization. The correction these additive interferences--in particular for complex isotopic systems--is numerically challenging still emerging field research. When positional information is generated via collision-induced dissociation, even...
For the first time an analytical work flow based on accurate mass gas chromatography-quadrupole time-of-flight spectrometry (GC-QTOFMS) with chemical ionization for analysis providing a comprehensive picture of (13)C distribution along primary metabolism is elaborated. The method provides powerful new toolbox (13)C-based metabolic flux analysis, which emerging strategy in engineering. In this field, stable isotope tracer experiments on, example, are central characteristic patterns labeled...
Synthetic water-soluble polymeric materials are widely employed in e.g. cleaning detergents, personal care products, paints or textiles. Accordingly, these compounds reach sewage treatment plants and may enter receiving waters the aquatic environment. Characteristically, molecules show a polydisperse molecular weight distribution, comprising multiple repeating units, i.e. homologous series (HS). Their analysis environmentally relevant samples has received some attention over last two...
Abstract Non-target analysis (NTA) employing high-resolution mass spectrometry is a commonly applied approach for the detection of novel chemicals emerging concern in complex environmental samples. NTA typically results large and information-rich datasets that require computer aided (ideally automated) strategies their processing interpretation. Such do however raise challenge reproducibility between within different workflows. An effective strategy to mitigate such problems implementation...
Within the plant kingdom, there is an exceptional amount of chemical diversity that has yet to be annotated. It for this reason non-targeted analysis interest those working in novel natural products. To increase number and compounds observable root exudate extracts, several workflows which differ at three key stages were compared: 1) sample extraction, 2) chromatography, 3) data preprocessing. Plants grown Hoagland's solution two weeks, exudates initially extracted with water, followed by a...
The wide chemical diversity and complex matrices inherent to metabolomics still pose a challenge current analytical approaches for metabolite screening. Although dedicated front-end separation techniques combined with high-resolution mass spectrometry set the benchmark from an point of view, increasing number samples sample complexity demand compromise in terms selectivity, sensitivity high-throughput analyses.
Abstract Root exudation is a major pathway of organic carbon input into soils. It affects soil physical properties, element solubility as well speciation, and impacts the microbial community in rhizosphere. exudates contain large number primary secondary plant metabolites, amount composition are highly variable depending on species developmental stage. Detailed information about exudate will allow for better understanding exudate-driven rhizosphere processes their feedback loops. Although...
A novel analytical approach based on liquid chromatography coupled to quadrupole time of flight mass spectrometry, employing data-dependent triggering for analysis isotopologue and tandem isotopomer fractions metabolites the primary carbon metabolism was developed. The implemented QTOFMS method employs automated MS/MS higher abundant, biologically relevant isotopologues generating positional information respective metabolite. Using this advanced selective fragmentation enables generation...
In the field of metabolic engineering 13C-based flux analysis experiments have proven successful in indicating points action. As every step this approach is affected by an inherent error, aim present work comprehensive evaluation factors contributing to uncertainty nonnaturally distributed C-isotopologue abundances as well absolute value calculation. For purpose, a previously published data set, analyzed course 13C labeling experiment studying glycolysis and pentose phosphate pathway yeast...
N-Acetylglucosamine is a key component of bacterial and fungal cell walls the extracellular matrix animal cells. It plays variety roles at surface structure under discussion to be involved in signaling pathways. The presence number N-acetylhexosamine stereoisomers samples biological or biotechnological origin demands for dedicated high efficiency separation methods, due identical exact mass similar fragmentation patterns stereoisomers. Gas chromatography offers sample capacity, efficiency,...
Abstract Root exudates are composed of primary and secondary organic compounds that function as signalling molecules play important roles in plant-environment interactions. The quantity composition root vary depending on the species, genotype, environmental conditions, including presence identity neighbouring plants. Although cover crops commonly used agricultural practices for their ecosystem services, such weed suppression, pathogen control, soil structure improvement, studies limited. Our...