A5036459630- Enzyme Structure and Function
- Bacterial Identification and Susceptibility Testing
- Protein Structure and Dynamics
- Advanced Electron Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Light effects on plants
- Biochemical and Molecular Research
- Computational Drug Discovery Methods
- Photochromic and Fluorescence Chemistry
- RNA modifications and cancer
- Porphyrin Metabolism and Disorders
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
Johannes Gutenberg University Mainz
2025
Koç University
2021-2023
X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this to unravel mechanistic details biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid or large compartments). Since its inception, single-crystal cryocrystallography has never been performed in Türkiye due the lack diffractometer. The diffraction facility recently established at...
The SARS-CoV-2 main protease of (Mpro) is an important target for related drug repurposing and development studies. Here, we describe the steps structural characterization Mpro, starting from plasmid preparation protein purification. We detail crystallization using sitting drop, microbatch (under oil) approach. Finally, cover data collection structure determination serial femtosecond crystallography. For complete details on use execution this protocol, please refer to Durdagi et al. (2021).
Since early 2020, COVID-19 has grown to affect the lives of billions globally. A worldwide investigation been ongoing for characterizing virus and also finding an effective drug developing vaccines. As time essence, a crucial part this research repurposing; therefore, confirmation in silico screening studies have carried out purpose. Here we demonstrated possibility variety drugs efficiently by leveraging high data collection rate 120 images/second with new low-noise, dynamic range ePix10k2M...
Abstract X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this to unravel mechanistic details biological macromolecules (e.g. proteins, nucleic acids, protein complexes, protein-nucleic acid or large compartments). Since its inception, single-crystal cryo-crystallography has never been performed in Türkiye due the lack diffractometer. The diffraction facility recently established...
Photolyases (PLs) reverse UV-induced DNA damage using blue light as an energy source. Of these PLs, (6-4) PLs repair (6-4)-lesioned photoproducts. We recently identified a gene from Vibrio cholerae (Vc) encoding PL, but structural characterization is needed to elucidate specific interactions with the chromophore cofactors. Here, we determined crystal structure of Vc PL at 2.5 Å resolution. Our high-resolution revealed that two well-known cofactors, flavin adenine dinucleotide and...
Abstract Photolyases (PLs) reverse UV-induced DNA damage using blue light as an energy source. Of these photolyases, (6-4) PLs repair (6-4)-lesioned photoproducts. We recently identified a gene from Vibrio cholerae (Vc ) encoding PL, but structural characterization is needed to elucidate specific interactions with the chromophore cofactors. Here, we determined crystal structure of Vc PL at 2.5 Å resolution. Our high-resolution revealed that two well-known cofactors, flavin adenine...
Abstract Since early 2020, COVID-19 has grown to affect the lives of billions globally. A worldwide investigation been ongoing for characterizing virus and also finding an effective drug developing vaccines. As time essence, a crucial part this research repurposing; therefore, confirmation in-silico screening studies carried out purpose. Here we demonstrated possibility variety drugs efficiently by leveraging high data collection rate 120 images/second with new low-noise, dynamic range...