We investigated the prevalence of intestinal protozoan parasites in patients with gastrointestinal complaints medical centers Zahedan, Iran. A total 1562 stool samples was examined from July 2004 to January 2006 using microscopy (direct smear, formalin-ether concentration), xenic culture and PCR techniques. Four hundred twenty-seven (27.3%) were infected one or more parasites. Giardia lamblia (10.1%), Entamoeba coli (10%), E. hartmanni (1.7%), Blastocystis hominis (2.2%), Chilomastix mesnili...

Introduction: Intestinal parasitic infections (IPIs) are important causes of morbidity and mortality in patients with immunocompromising conditions.Objective: The aim this study was to determine the prevalence IPIs different groups immunocompromised patients, including hemodialysis (HD), renal transplant recipients (RTR), cancer HIV/AIDS comparison healthy individuals two central cities Iran (Kashan Qom).Methods: In case–control study, stool samples 135 HD, 50 RTR, 60 20 120 subjects were...

Abstract ToRCH infections (toxoplasmosis, rubella, cytomegalovirus and Herpes simplex virus) have long been known to be associated with bad obstetric outcomes. However, little information is available about the impact of co‐infections on outcome pregnancy. Hence, we tested IgG IgM antibodies T oxoplasma gondii , Rubella, Cytomegalovirus Simplex Virus among 81 pregnant women abortion (case group) 98 normal delivery (control group). In single‐infection model, only CMV ‐ seropositivity was...

Objectives: This study was aimed to evaluate the role of toxoplasmosis in etiology abortion and stillbirth based on molecular serological techniques.Material methods: A total 110 pregnant women with were enrolled as case group, normal delivery control group. Serological detections Toxoplasma gondii assessed by ELISA PCR methods.Results: The seroprevalence IgG 25.5% group (26.8% 21.4% stillbirth) 26.4% IgM seropositivity detected 2.7% (3.6% 0% 0.9% (p = 0.37). DNA 6.4% (7.3% 3.6% 1.8% 0.17)....

Cutaneous leishmaniasis (CL) is endemic in the northwest of Isfahan province, Iran. Increase incidence disease Kashan has made it necessary to find out best method for diagnosis and molecular characterization Leishmania species. In present study, 130 patients suspected cutaneous leishmaniosis referred health care centers were examined. Serosity lesion was collected smear preparation cultured Novy‐Nicolle‐McNeal medium. DNA extracted from serosity, species determined by polymerase chain...

Toxoplasma gondii is one of the important opportunistic pathogen among solid-organ transplant recipients and hemodialysis patients (HD). This study was aimed to detect toxoplasmosis 50 renal (RTR), 135 HD 120 healthy individuals in two cities (Kashan Qom) that located center Iran, from 2014 2015. Serological detection (IgG IgM antibodies) performed all case control groups. Molecular on positive or IgG with moderate high (>51 IU/mL) antibody titers (n = 42) groups 21). In RTR patients,...

: Background: Swimming pools can transmit contagious diseases such as; fungal disease, otitis, conjunctivitis and amoebic meningoencephalitis.Objectives: The aim of this study was to determine the level microbial contamination physicochemical condition swimming pools.Materials Methods: In cross – sectional study, 200 water samples were collected from four in Kashan, Iran. Temperature, pH residual chlorine turbidity examined. Samples concentrated through a membrane filter sedimentation, test...

Implication for health policy/practice/research/medical education: Low birth weight could be considered as risk factor of Candida sp.colonization and the screening mothers with preterm labor proper management helps to avoid colonization subsequent invasive candidiasis.

1 Department of Parasitology, School Medicine, Kashan University Medical Sciences, Kashan, IR Iran2 Qazvin Qazvin, Iran3 Microbiology, Public Health, Tehran Tehran, Iran

Abstract Toxoplasmosis is one of the most common meat‐borne parasitic infections worldwide. Consumption raw or undercooked meat which contains Toxoplasma gondii tissue cysts an important route human infection. In this study, we investigate serological and molecular prevalence T. infection in sheep goat samples Kashan, Iran, from 2015 to 2016. Serological (IgG antibody) detections were performed by enzyme‐linked immunosorbent assay polymerase chain reaction on sera heart ( n = 90) goats 90),...

Cutaneous leishmaniasis is a common skin disease caused by leishmania parasite. An accurate diagnosis of parasites species possible using molecular techniques. This study was carried out to compare internal transcribed spacer (ITS1) and kinetoplast deoxyribonucleic acid (KDNA) genes for identifying Leishmania Polymerase Chain Reaction (PCR), furthermore, genetic diversity isolates studied. research examined 130 patients who were suspected cutaneous referred Kashan's health centers from...

Background: Acanthamoeba ​ species are recognized as the most prevalent free‐living amoebae (FLA). They can cause granulomatous amebic encephalitis (GAE) and pulmonary ocular infections. The present study aimed to isolate identify genotypes in cancer patients referred Kashan’s hospitals Central Iran. Methods: This cross‐sectional was conducted with oral nasal swab samples collected from a hundred Beheshti Yasrebi during 2019–2020. were cultured 1.5% non‐nutrient agar (NNA) heat‐killed...

Background: Entamoeba histolytica/Entamoeba dispar and Enterobius vermicularis are the major health problems in developing countries especially Iran. The prevalence of infection is variable among different social groups world. Objectives: Since elderly mentally retarded high risk group, present survey was carried out order to determine intestinal parasites these residence Golabchi Center, Kashan, Materials Methods: In this Cross-sectional study a total 243 stool samples 279 Scotch tapes from...

Background:: There are scant evidences between giardiasis and enterobiasis with human mal-absorption of micronutrient. Objectives:: The aim the present study was to found out changes in serum zinc, copper, magnesium vitamin B12 levels schoolchildren infected Giardia intestinalis Enterobius vermicularis. Patients Methods:: This case-control carried among 359 children from aged 6 - 12 years old at 8 suburban public schools. Three stool examinations were done using standard techniques for...

Background:: Congenital toxoplasmosis (CT) is the result of maternal infection if acquired during gestation, an that most often clinically unapparent. Objectives:: This study was conducted to determine acute and chronic toxoplamosis in pregnant women its complications newborn. Patients Methods:: In this cohort study, 798 were tested for Toxoplasma IgG/IgM by ELISA. PCR IgM performed on 4 newborns 5 infections; they followed 1 year. Data analyzed SPSS-11.5 χ2 test. Results:: Five (0.63%) 341...

Both zoonotic cutaneous leishmaniasis (ZCL) and anthroponotic are endemic in different regions of Iran. It is important to know the type (CL) for prevention strategies. On other hand, Iran one countries that was severely affected by coronavirus 2019 (COVID-19) pandemic. However, little known about causative agents CL during COVID-19 pandemic Iran.In this study, a total 181 samples were isolated from patients with lesions four cities (Jahrom, Juyom, Kashan Shoushtar) (the summer autumn 2021)....

The nucleotide sequences of Serine-Rich Entamoeba histolytica Protein (SREHP) gene have already exhibited stable and significant polymorphism in the studies. Serine-rich protein is also present polymorphic dispar which called SREDP. (SREDP) among 8 isolates obtained from Iranian cyst carriers were analyzed by a nested PCR-RFLP followed sequencing PCR products. From those isolates, six distinct DNA patterns observed after PCR, whereas showed different isolates. results demonstrate an...

Background: Acanthamoeba is one of the most common opportunistic free-living amoebae, with ubiquitous presence in various environmental sources. Pathogenic strains are causative agents amoebic keratitis and granulomatous encephalitis. Objectives: The aim present study was to identify genotypes soil, hospital dust, stagnant water samples from Kashan, Central Iran. Methods: In this cross sectional study, a total 122 soil (n, 32), dust 40), 50) were collected examined for amoebae species. All...

Dear Editor, Infection with Toxoplasma gondii during pregnancy may cause severe damages to the fetus including spontaneous abortion, stillbirth and neurological signs. Its severity is gestational time-dependent (1). According previous studies, maternal infection in first second trimester be resulted prenatal death or 5% 2 % of cases, respectively (1, 2). The major diagnostic assays for toxoplasmosis are serological methods despite many limitations (3). Molecular method have been...