This prospective cross-sectional study was aimed to evaluate the prevalence of IgM and IgG anti-T. gondii antibodies associated risk factors among healthy blood donors in Kerman province, south-eastern Iran. Structured questionnaires (before gave blood) were used obtain information on for infection. Totally, 500 serum samples from Blood Transfusion Organization (KBTO) at Kerman, Iran, screened by enzyme-linked immunosorbent assay (ELISA) Roche Elecsys Toxo assay. Real-time PCR detect DNA T....

Surgery remains the preferred treatment for hydatid cyst (cystic echinococcosis, CE). Various scolicidal agents have been used inactivation of protoscolices during surgery, but most them are associated with adverse side effects. The present study aimed to evaluate in vitro effect Nigella sativa (Ranunculaceae) essential oil and also its active principle, thymoquinone, against cysts. Protoscolices were aseptically aspirated from sheep livers having concentrations (0.01-10 mg/ml) thymoquinone...

Blastocystis is a zoonotic protozoan parasite living in the digestive system of some vertebrates. This has subtypes, pathogenicity status which still remained controversial.The aim this study was to determine subtype infected cattle.This descriptive cross-sectional performed on 196 isolates from cattle stool samples collected slaughterhouse Khorramabad city, Iran, 2012. Genomic DNA extracted and subtype, seven pairs sequence-tagged sites (STS) primers were used.Of specimens, 19 (9.6%) with...

Parasitic infection is one of the problems that affect human health, especially in developing countries. In this study, all fast food shops, restaurants, and roast meat outlets Khorramabad (Western Iran) staff employed by them, some 210 people, were selected through a census their stools examined for presence parasites. The parasitological tests direct wet-mount, Lugol's iodine staining, formaldehyde-ether sedimentation Trichrome staining techniques performed on samples. data was analyzed...

As the new pandemic created by COVID-19 virus need of rapid acquisition a suitable vaccine against SARS-CoV-2 to develop Immunity and reduce mortality, aim this study was identify S protein N antigenic epitopes using immunoinformatic methods design SARS-CoV-2, for which protein-dependent are predicted. B cell, CTL HTL were determined based on antigenicity, allergenicity toxicity that non-allergenic, non-toxic, selected multi-epitope structure. Then, in order increase safety Hbd-3 Hbd-2 as...

The present study was designed to evaluate the chemical composition and scolicidal effects of Pistacia atlantica Desf. extract against protoscoleces hydatid cysts its acute toxicity in mice model. Various concentrations methanolic (5-50 mg/mL) were used for 10-60 min. Viability confirmed using eosin exclusion test (0.1%). Acute also determined main components β-myrcene (41.4%), α-pinene (32.48%) limonene (4.66%). Findings demonstrated that P. at 25 50 mg/mL after 20 10 min exposure killed...

This study aims to evaluate the in vitro and vivo antileishmanial activities of Pistacia vera essential oil compare their efficacy with a reference drug, meglumine antimoniate (Glucantime®). (0-100 µg/mL) was evaluated against intracellular amastigote forms Leishmania tropica (MHOM/IR/2002/Mash2) then tested on cutaneous leishmaniasis male BALB/c mice by major (MRHO/IR/75/ER). In assay, it could be observed that P. significantly (p < 0.05) inhibited growth rate (IC50 21.3 ± 2.1...

Current scolicidal agents, which have been used for inactivation of protoscoleces during surgical procedures, are associated with adverse side effects including sclerosing cholangitis. This investigation aimed to evaluate the Bunium persicum (Boiss) essential oil against hydatid cysts and also its toxicity in a mice model.Protoscoleces were aseptically aspirated from sheep livers having cysts. Various concentrations (3.125-50 mcL/mL) 5-30 min. The viability was confirmed using eosin...

Cutaneous leishmaniasis (CL) is endemic in the northwest of Isfahan province, Iran. Increase incidence disease Kashan has made it necessary to find out best method for diagnosis and molecular characterization Leishmania species. In present study, 130 patients suspected cutaneous leishmaniosis referred health care centers were examined. Serosity lesion was collected smear preparation cultured Novy‐Nicolle‐McNeal medium. DNA extracted from serosity, species determined by polymerase chain...

To study the prevalence of intestinal parasites in Lorestan Province, West Iran. This cross-sectional was conducted on 2 838 stool samples Khorramabad, Province 2013. Samples were examined by several techniques. The frequency 465 (16.4%) which 188 (13.5%) for urban areas and 277 (19.2%) rural areas. Infection significantly higher than Out infected samples, 456 (98%) contaminated with protozoan 9 (2%) helminthes. people who sometimes used soap to wash hands more those always (P<0.001). poor...

To identify Leishmania using PCR.This study was conducted from April 2009 to March 2011 in order species a new endemic area of CL Lorestan, Iran. Samples were taken 62 patients that referred the health centers different cities Lorestan province, presence confirmed direct smear and then grown NNN media mass cultured RPMI 1 640 medium supplemented with 10% heat-inactivated fetal bovine serum. DNA extracted promastigotes used ITS-PCR.45(72.6%) samples out showed band range 485 bp 17 (27.4%) 626...

Background: Toxoplasmosis is one of the most important diseases in humans and animals. Almost one-third human population around world infected with toxoplasmosis. The agent this parasitic disease a protozoan called Toxoplasma gondii (T. gondii) that causes encephalitis people suppressed immune systems abortion, mental retardation chorioretinitis fetus. Alzheimer's (AD) neurodegenerative disease. Objectives: Due to high prevalence toxoplasmosis Iran evidence on its impact diseases, study was...

Background: The present work aimed to evaluate the chemical composition of Curcuma zadoaria essential oil and investigate its efficacy safety against hydatid cyst protoscoleces. Methods: Collected protoscoleces from liver fertile cysts infected sheep were exposed different concentrations (75, 150, 300 μl/mL) for 5-30 min in vitro ex vivo. Then, by using eosin exclusion assay, viability was studied. In next step, 24 male NMRI mice examined assess toxicity C. measuring biochemical...

Abstract Fascioliasis is a parasitic infection caused by Fasciola spp. in humans and animals. Despite significant advances vaccination new therapeutic agents, little attention has been paid to validate methods for the diagnosis of fascioliasis This study aimed compare loop‐mediated isothermal amplification (LAMP) technique with PCR assay F. hepatica sheep. In this cross‐sectional study, 195 stool samples were collected from sheep 3 months Lorestan province, West Iran. Specimens’...

Abstract Currently, zinc oxide (ZnO) particles are used in nanotechnology to destroy a wide range of microorganisms. Although pentavalent antimony compounds as antileishmanial drugs, they associated with several limitations and side effects. Therefore, it is always desirable try find new effective treatments. The aim this research determine the effect ZnO comparison Antimoan Meglumine compound on promastigotes amastigotes Leishmania major (MRHO/IR/75/ER). After extraction purification...